Estudo da expressão de ligantes e receptores de matriz extracelular nas células endoteliais tímicas e sua participação na migração

Detalhes bibliográficos
Ano de defesa: 2008
Autor(a) principal: Francelino, Andrea Aragao
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Alagoas
BR
Programa de Pós-Graduação em Ciências da Saúde
UFAL
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.ufal.br/handle/riufal/922
Resumo: The traffic of T cells, that occur when bone marrow-derived T cell precursors penetrate the thymus, with a phenotype of immature cells, and its subsequent output, as mature cells, depends of the thymic blood vessels that consists mainly of thymic endothelial cells. The expression of ligands and receptors of ECM in thymic endothelial cells, as well as its role in the processes of migration and emigration of the T cells from the thymus remains unclear. Our goal was to evaluated the expression of some of ECM ligands and their respective receptors in the strain of thymic endothelial cells tEnd.1. In addition, we aimed to study the role of the components of the ECM in the tEnd.1 cells and thymocytes interaction. First, it was detected by confocal immunofluorescence the presence of ECM proteins fibronectin and laminin, which were also observed through flow cytometry. The presence of their respective receptors, VLA5 and VLA6, were highlighted by immunoperioxidase and flow cytometry. Assays of adhesion showed that the use of antibodies anti-VLA5 and anti-VLA6 seems to block at least partially, the adhesion of thymocytes to endothelial cells. In addition, the transendothelial migration assay showed less migration in absolute cell number of the thymocytes pre-treated with antibodies anti-receptors of ECM, although this migration was not statistically significant. Finally, when the phenotype of the transmigrated thymocytes was observed, there was not difference in the migration pattern among them. This investigation shows that the thymic endothelial cells are able to express fibronectin and laminin proteins, as well as, their respective receptors VLA5 and VLA6, and suggests that these molecules could participate in the processes of intrathymic lymphocytes migration.