Desenvolvimento de metodologia espectrofotométrica multivariada para o controle de qualidade da associação acido kojico e hidroquinona em dermocosméticos

Detalhes bibliográficos
Ano de defesa: 2010
Autor(a) principal: Calaça, Giselle Nathaly
Orientador(a): Nagata, Noemi lattes
Banca de defesa: Pessoa, Christiana Andrade lattes, Peralta-zamora, Patricio Guillermo lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: UNIVERSIDADE ESTADUAL DE PONTA GROSSA
Programa de Pós-Graduação: Programa de Pós-Graduação em Química Aplicada
Departamento: Química
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://tede2.uepg.br/jspui/handle/prefix/2078
Resumo: Kojic Acid (KA) and Hydroquinone (HQ) are depigmenting agents used as skinwhitening cosmetics to treat dyschromias. The need for low cost analytical methodologies that enable quality control of this association is evident. Due to the characteristics such as high sensitivity, low cost and operational simplicity, this work aims to use spectrophotometry in the visible region and multivariate calibration tools, mainly Partial Least Squares Regression (PLSR) for quantitative etermination of KA and HQ. The method is based on the complexation of kojic acid with Fe3+ ion (λ = 494nm), while hydroquinone reduces Fe3+ ions to Fe2+, which complex with 1,10-phenanthroline (λ = 510nm). The quantitative determination of KA and HQ by conventional methods was performed by analytical curve, spectrophotometric additivity principle and first-derivative pectrophotometry, and the average errors were: 20,65% (HQ) and 469,91% (AK), 41,44% (HQ) and 47,11% (AK), 57,59% (HQ) and 156,72% (AK), respectively. The results of conventional methods were compared to PLSR, and the multivariate models with better predictive capacity used: spectral range from 350 to 800 nm, mean center data, 03 and 02 latent variables for kojic acid and hydroquinone, respectively. The mean relative errors obtained from these models were: 4,20% for AK and 6,05% for HQ, indicating that PLSR method showed better results for the quantification of analytes, when compared to conventional methods. The multivariate methodology developed was validated according to ANVISA criteria and then used for quantification of analytes in real samples, obtained from manipulation pharmacies of the region.