Avaliação farmacognóstica e otimização da extração de flavonoides de flores de Tagetes patula através de planejamento de misturas

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Munhoz, Vanessa Marquito
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual de Maringá
Brasil
Departamento de Farmácia
Programa de Pós-Graduação em Ciências Farmacêuticas
UEM
Maringá, PR
Centro de Ciências da Saúde
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://repositorio.uem.br:8080/jspui/handle/1/1918
Resumo: Tagetes patula L. (Asteraceae), commonly known as marigold, has its flowers used in folk medicine as an antiseptic, diuretic, purifying the blood and insect repellent, as well as widely used as ornamental. This study was conducted to develop the pharmacognostic evaluations for T. patula flowers, as well as to optimize the extraction process, based on the total flavonoid content, through statistical mixture design simplex-centroid, which allowed to evaluate the effect of the solvents water, ethanol and acetone, as well as mixtures thereof, on this response. Also were tested dry residue and antioxidant capacity of the extracts produced. These evaluations were performed according to the Brazilian Pharmacopoeia and/or specialized literature. Colorimetric assays detected the presence of flavonoids, tannins and simple phenolics. By thin layer chromatography and high performance liquid chromatography it was possible to determine the presence of the substance quercetin. The results for loss on drying, total ash content, extractives content and total flavonoid content were, respectively: 9.35%±0.2 (2.10%), 5.51%±0.2 (3.61%), 39.55%±0.52 (1.31%) e 5.24%±0.08 (1.51%). The extract with acetone had the highest total flavonoid content 25.13%±1.02 (4.07%) and better antioxidant capacity, with IC50 of 15.74 μg/mL±1.09 (6.92%), but with lower dry residue, 6.62%±1.33 (20.10%). The extracts containing water as extractor liquid exhibited higher values of dry residue, but with the total flavonoid content and antioxidant capacity smaller than extract with acetone. The correlation between the total flavonoid content and antioxidant capacity of the extracts showed that flavonoids contribute significantly to this capacity. Thus, the statistical mixture design allowed the optimization of the extraction of flavonoids from the flowers of T. patula, with acetone as the best liquid extractor.