Análise do perfil proteômico do tecido gorduroso de lagartas de Bombyx mori L. (Lepidoptera: Bombycidae) inoculadas com nucleopolyhedrovirus (BmNPV)

• Ano de defesa: 2015
• Autor(a) principal: Chiarello, Alex Sandro Gularte
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual de Maringá; Brasil; Programa de Pós-Graduação em Genética e Melhoramento; UEM; Maringá, PR; Centro de Ciências Agrárias
• Palavras-chave em Português: Proteôma; Bicho da seda (Bombyx mori L.); Resposta imune; Nucleopolyhedrovirus (BmNPV); Brasil.; Proteome; Immune response; Nucleopolyhedrovirus; Brazil.; Ciências Agrárias; Agronomia
• Link de acesso: http://repositorio.uem.br:8080/jspui/handle/1/1365

The Bombyx mori L. or silkworm silk as it is popularly known is a body of great economic importance to man since antiquity. B. mori is a model organism in which several genes have been identified for defense proteins with antimicrobial activity. The detection and sequencing of proteins for defense against pathogens such as BmNPV becomes a tool for understanding the biology of the organism and an improvement in breeding and increased yields in cocoons, because many losses are associated with infection by pathogens. BmNPV is the virus of the biggest losses for sericicultores to change various physiological characteristics of the animal throughout infection. The presence of the virus triggers a cascade of metabolic reactions that are intended to eliminate the virus multiplication and migration of the virus to adjacent tissue. The fat body is the main site of synthesis of defense against microorganism?s proteins in insects, thus employing proteomic research tools crawlers inoculated with BmNPV can reveal the state of gene expression in specific physiological conditions during the infectious process in the fat body. This study aimed to identify differences in the pattern of protein expression in infected larvae of the seventh and eighth day of the 5th ínstar and verify that they are related to self-defense process and also sequence the protein kin 17 based on references on the isoelectric point and molecular weight. The extracted fat body total protein were analyzed by 2-DE and electrophoresis was carried sequencing of tryptic fragments by mass spectrometry (MALDI / TOF-TOF) of some differentially expressed spots. The results point to a difference in the expression pattern of larvae inoculated with BmNPV proteins when compared to the fat tissue samples of caterpillar control during normal lepidopteran development. Of the eleven cut spots in the gel 2-DE and trypsinized, only three were identified: number 6 spot sample 7ºDPI identified as elongation factor 1-alpha, the spot 8, the eighth day control sample identified factor imaginal disc growth and the spot 11, sample 8 DPI identified a non-characterized protein of B. mori. Proteins analyzed in this study are probably involved in metabolic functions in the cells of the fat body, however, the involvement of these proteins in the immune system of Bombyx mori can not be determined.