Atividade imunoestimulante e anti-Leishmania de Chenopodium ambrosioides L. (mastruz)

• Ano de defesa: 2011
• Autor(a) principal: Patrício, Fernando José Brito
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: UNIVERSIDADE FEDERAL DO MARANHÃO
• Palavras-chave em Português: Biotecnologia; Leishmania amazonensis; Macrofagos - Ativacao; Óxido nítrico
• Link de acesso: https://siduece.uece.br/siduece/trabalhoAcademicoPublico.jsf?id=71022

<span class="markedContent" id="page67R_mcid2"><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*146.32px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.915324);" role="presentation" dir="ltr">Chenopodium ambrosioides</span><span style="left: calc(var(--scale-factor)*227.57px); top: calc(var(--scale-factor)*146.32px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.926833);" role="presentation" dir="ltr">, conhecida popularmente como mastruz, mastruço, menstruço </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*166.96px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.901922);" role="presentation" dir="ltr">ou erva de Santa Maria é uma das plantas mais  utilizadas para o tratamento de leishmaniose</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*187.72px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.923628);" role="presentation" dir="ltr">por populações de áreas endêmicas do nordeste brasileiro. O presente estudo investigou o</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.922623);" role="presentation" dir="ltr">potencial  imunoestimulador e anti-</span><span style="left: calc(var(--scale-factor)*259.37px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.912687);" role="presentation" dir="ltr">Leishmania</span><span style="left: calc(var(--scale-factor)*315.40px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*318.79px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.848201);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*330.12px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*333.43px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.911424);" role="presentation" dir="ltr">C. ambrosioides</span><span style="left: calc(var(--scale-factor)*412.54px); top: calc(var(--scale-factor)*208.36px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.927031);" role="presentation" dir="ltr">. O efeito do extrato bruto</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*229.15px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.940309);" role="presentation" dir="ltr">hidroalcoólico (70%) (HCE) foi avaliado sobre o número de  células  linfóides e ativação de</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*249.79px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.978925);" role="presentation" dir="ltr">macrófagos. Camundongos C3H/HePas foram tratados via intraperitoneal com HCE e</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*270.55px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.909332);" role="presentation" dir="ltr">sacrificados 48 horas depois. O HCE aumentou o número de células na cavidade peritoneal,</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*291.19px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.909766);" role="presentation" dir="ltr">baço e linfonodo, mas não na medula óssea. Os macrófagos dos animais tratados  com HCE</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*311.83px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.889255);" role="presentation" dir="ltr">aumentaram a capacidade de espraiamento, a atividade fagocítica, a produção de peróxido de</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.952557);" role="presentation" dir="ltr">hidrogênio (H</span><span style="left: calc(var(--scale-factor)*159.38px); top: calc(var(--scale-factor)*337.15px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">2</span><span style="left: calc(var(--scale-factor)*163.49px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">O</span><span style="left: calc(var(--scale-factor)*172.13px); top: calc(var(--scale-factor)*337.15px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">2</span><span style="left: calc(var(--scale-factor)*176.21px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.982574);" role="presentation" dir="ltr">) e óxido nítrico (NO)</span><span style="left: calc(var(--scale-factor)*285.64px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*289.75px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.928458);" role="presentation" dir="ltr">ex  vivo</span><span style="left: calc(var(--scale-factor)*324.43px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.927015);" role="presentation" dir="ltr">. O tratamento</span><span style="left: calc(var(--scale-factor)*395.54px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*399.70px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.02088);" role="presentation" dir="ltr">in vitro</span><span style="left: calc(var(--scale-factor)*435.78px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*439.90px); top: calc(var(--scale-factor)*332.59px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.962778);" role="presentation" dir="ltr">com o HCE induziu</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*353.23px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.915897);" role="presentation" dir="ltr">aumento dose-dependente na produção de NO em macrófagos residentes. A atividade anti-</span>L<span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.912817);" role="presentation" dir="ltr">eishmania</span><span style="left: calc(var(--scale-factor)*146.78px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*154.10px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05168);" role="presentation" dir="ltr">foi</span><span style="left: calc(var(--scale-factor)*167.43px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*174.73px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.894136);" role="presentation" dir="ltr">avaliada</span><span style="left: calc(var(--scale-factor)*214.71px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*221.97px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.876788);" role="presentation" dir="ltr">em</span><span style="left: calc(var(--scale-factor)*236.59px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*243.92px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.91998);" role="presentation" dir="ltr">um</span><span style="left: calc(var(--scale-factor)*259.26px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*266.59px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.914444);" role="presentation" dir="ltr">modelo</span><span style="left: calc(var(--scale-factor)*302.59px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*309.90px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.848201);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*321.22px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*328.48px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.90936);" role="presentation" dir="ltr">infecção</span><span style="left: calc(var(--scale-factor)*369.14px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*376.45px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.911417);" role="presentation" dir="ltr">experimental</span><span style="left: calc(var(--scale-factor)*439.08px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*446.62px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01339);" role="presentation" dir="ltr">in</span><span style="left: calc(var(--scale-factor)*456.09px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*463.40px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.934522);" role="presentation" dir="ltr">vivo</span><span style="left: calc(var(--scale-factor)*483.34px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*490.68px); top: calc(var(--scale-factor)*374.01px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.948079);" role="presentation" dir="ltr">utilizando</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.88283);" role="presentation" dir="ltr">camundongos</span><span style="left: calc(var(--scale-factor)*157.32px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*164.76px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.895193);" role="presentation" dir="ltr">C3H/HePas</span><span style="left: calc(var(--scale-factor)*221.48px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*229.01px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">e</span><span style="left: calc(var(--scale-factor)*234.34px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*241.85px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.912491);" role="presentation" dir="ltr">Leishmania</span><span style="left: calc(var(--scale-factor)*297.87px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*305.29px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.867915);" role="presentation" dir="ltr">amazonensis</span><span style="left: calc(var(--scale-factor)*366.79px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">.</span><span style="left: calc(var(--scale-factor)*369.79px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*377.22px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">O </span><span style="left: calc(var(--scale-factor)*393.29px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.894527);" role="presentation" dir="ltr">tratamento</span><span style="left: calc(var(--scale-factor)*444.61px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*452.26px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.909429);" role="presentation" dir="ltr">com</span><span style="left: calc(var(--scale-factor)*472.88px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*480.33px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr">o</span><span style="left: calc(var(--scale-factor)*486.33px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*493.76px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.947328);" role="presentation" dir="ltr">HCE</span><span style="left: calc(var(--scale-factor)*517.76px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*525.36px); top: calc(var(--scale-factor)*394.65px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05168);" role="presentation" dir="ltr">foi </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*415.41px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.949105);" role="presentation" dir="ltr">realizado entre a 4</span><span style="left: calc(var(--scale-factor)*185.21px); top: calc(var(--scale-factor)*412.89px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">a</span><span style="left: calc(var(--scale-factor)*188.78px); top: calc(var(--scale-factor)*412.89px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*193.97px); top: calc(var(--scale-factor)*415.41px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.977152);" role="presentation" dir="ltr">e 6</span><span style="left: calc(var(--scale-factor)*210.41px); top: calc(var(--scale-factor)*412.89px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr">a</span><span style="left: calc(var(--scale-factor)*213.98px); top: calc(var(--scale-factor)*412.89px); font-size: calc(var(--scale-factor)*8.04px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*219.05px); top: calc(var(--scale-factor)*415.41px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.946745);" role="presentation" dir="ltr">semanas pós-infecção, administrado via oral ou intralesional. O</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*436.05px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.913272);" role="presentation" dir="ltr">tratamento oral não teve efeito na infecção. Já o tratamento via intralesional induziu discreto </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*456.81px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.89622);" role="presentation" dir="ltr">aumento na espessura da pata e reduziu a carga parasitária no linfonodo drenante, no baço e</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*477.45px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.913371);" role="presentation" dir="ltr">na pata infectada. Este efeito protetor foi acompanhado pelo aumento na produção de óxido</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*498.21px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.917206);" role="presentation" dir="ltr">nítrico em culturas de células de linfonodo e peritônio. Por fim, as frações hexânica (FHEX), </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*518.88px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.952812);" role="presentation" dir="ltr">clorofórmica (FCl), acetato de etila (FAc) e a fração hidroalcoólica (FHA) foram obtidas a</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*539.64px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.04959);" role="presentation" dir="ltr">partir do HCE a fim de identificar os compostos ativos. As frações FHEX e FCL </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*560.28px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.910671);" role="presentation" dir="ltr">apresentaram efeito citotóxico sobre células murinas que não foi observado  nas frações FAc</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*581.04px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.921025);" role="presentation" dir="ltr">e FHA. Estas frações não citotóxicas apresentaram significante atividade antipromastigota,</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*601.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.97107);" role="presentation" dir="ltr">inibiram a proliferação de amastigotas  no modelo de infecção</span><span style="left: calc(var(--scale-factor)*408.22px); top: calc(var(--scale-factor)*601.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*413.98px); top: calc(var(--scale-factor)*601.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.06501);" role="presentation" dir="ltr">in vitro</span><span style="left: calc(var(--scale-factor)*451.62px); top: calc(var(--scale-factor)*601.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*457.42px); top: calc(var(--scale-factor)*601.68px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.930289);" role="presentation" dir="ltr">e aumentaram a</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.01189);" role="presentation" dir="ltr">produção de NO em</span><span style="left: calc(var(--scale-factor)*200.11px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*207.44px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.927007);" role="presentation" dir="ltr">macrófagos peritoneias</span><span style="left: calc(var(--scale-factor)*323.10px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*330.55px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.99926);" role="presentation" dir="ltr">in</span><span style="left: calc(var(--scale-factor)*339.89px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*347.10px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.999419);" role="presentation" dir="ltr">vitro</span><span style="left: calc(var(--scale-factor)*369.79px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.05767);" role="presentation" dir="ltr">. A análise</span><span style="left: calc(var(--scale-factor)*429.07px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*436.30px); top: calc(var(--scale-factor)*622.44px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.942412);" role="presentation" dir="ltr">química demonstrou</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*643.08px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.916678);" role="presentation" dir="ltr">prevalência de  flavonoides, especialmente derivações glicosiladas de quercetina e canferol,</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*663.84px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.931077);" role="presentation" dir="ltr">nas duas frações ativas indicando que os efeitos biológicos  encontrados aqui podem estar</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*684.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.93658);" role="presentation" dir="ltr">relacionados a esses compostos.</span><span style="left: calc(var(--scale-factor)*256.96px); top: calc(var(--scale-factor)*684.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*264.17px); top: calc(var(--scale-factor)*684.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(1.00576);" role="presentation" dir="ltr">Os resultados ratificam o potencial medicinal de</span><span style="left: calc(var(--scale-factor)*520.47px); top: calc(var(--scale-factor)*684.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*527.64px); top: calc(var(--scale-factor)*684.50px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.919117);" role="presentation" dir="ltr">C.</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*705.26px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.905797);" role="presentation" dir="ltr">ambrosioides</span><span style="left: calc(var(--scale-factor)*155.40px); top: calc(var(--scale-factor)*705.26px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*159.62px); top: calc(var(--scale-factor)*705.26px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.910054);" role="presentation" dir="ltr">e fornecem  vidências promissoras para estudos posteriores em busca de um</span> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*725.90px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.890208);" role="presentation" dir="ltr">novo composto terapêutico derivado desta espécie vegetal.</span></span><span class="markedContent" id="page67R_mcid3"> <span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.881334);" role="presentation" dir="ltr">Palavras-chave: </span><span style="left: calc(var(--scale-factor)*177.17px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.940739);" role="presentation" dir="ltr">Ativação</span><span style="left: calc(var(--scale-factor)*220.48px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*231.05px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.848201);" role="presentation" dir="ltr">de</span><span style="left: calc(var(--scale-factor)*242.38px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*252.88px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.891641);" role="presentation" dir="ltr">macrófagos,</span><span style="left: calc(var(--scale-factor)*311.76px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*322.39px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.962969);" role="presentation" dir="ltr">Óxido</span><span style="left: calc(var(--scale-factor)*352.58px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*363.07px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.952608);" role="presentation" dir="ltr">nítrico,</span><span style="left: calc(var(--scale-factor)*397.38px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*407.98px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.910341);" role="presentation" dir="ltr">Leishmania</span><span style="left: calc(var(--scale-factor)*463.86px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif;" role="presentation" dir="ltr"> </span><span style="left: calc(var(--scale-factor)*474.44px); top: calc(var(--scale-factor)*746.66px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.868379);" role="presentation" dir="ltr">amazonensis, </span><span style="left: calc(var(--scale-factor)*90.74px); top: calc(var(--scale-factor)*767.30px); font-size: calc(var(--scale-factor)*12.00px); font-family: sans-serif; transform: scaleX(0.904373);" role="presentation" dir="ltr">Flavonóides. <br/></span></span>