REDUÇÃO DA UTILIZAÇÃO DE XILOL NO PROCESSAMENTO HISTOLÓGICO DE AMOSTRAS BIOLÓGICAS DE ANIMAIS PARA COMPARAÇÃO COM A TÉCNICA PADRÃO/CONVENCIONAL

• Ano de defesa: 2023
• Autor(a) principal: MIKOWSKI, ANDREIA
• Orientador(a): Kerppers, Ivo Ilvan
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Centro-Oeste
• Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina Veterinária (Mestrado)
• Departamento: Unicentro::Departamento de Medicina Veterinária
• País: Brasil
• Palavras-chave em Português: Coloração; Desparafinação; Meio ambiente; Poluição; Toxicidade
• Palavras-chave em Inglês: Coloring; Dewaxing; Environment; Pollution; Toxicity
• Área do conhecimento CNPq: CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://tede.unicentro.br:8080/jspui/handle/jspui/2110

Histological techniques help in the processing of biological samples applied to the study and diagnosis, using reagents and dyes that favor cell and tissue analysis. Sample preparation time is long and the use of potentially toxic chemical reagents expose handlers and generate environmental chemical residues, including xylene, an aromatic hydrocarbon widely used as a clarifying and dewaxing agent. This study aims to reduce the use of xylene in the final stage of hematoxylin and eosin (HE) staining compared to the standard protocol for staining in different tissue samples from necropsied animals, without losing sample quality and diagnosis. Sixty-six slides were made from 33 paraffin blocks, divided into two groups (n 33) and stained in HE with xylene and with reduction of without xylene. The slides were analyzed by 25 professionals from the biological and veterinary areas, and the results expressed by descriptive statistics, presenting the average values. The Spearman Coefficient correlation test was performed. In the statistical program Jamovi. Slides from the test group showed better static averages than those from the control group. Concluding that xylene can be reduced and excluded from the last staining step, maintaining structural quality of tissue and diagnosis, reducing operating costs and without harming the health of the worker.