PRODUÇÃO E CARACTERIZAÇÃO DE MICROENCAPSULADO DE EXTRATO DE Baccharis dracunculifolia E LASIODIPLODANA (16)--D-GLUCANA

• Ano de defesa: 2019
• Autor(a) principal: Iurckevicz, Genice
• Orientador(a): Cunha, Mário Antônio Alves da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Centro-Oeste
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química (Doutorado)
• Departamento: Unicentro::Departamento de Ciências Exatas e de Tecnologia
• País: Brasil
• Palavras-chave em Português: produtos naturais; planejamento experimental; bioatividade; encapsulação; nanopartículas
• Palavras-chave em Inglês: natural products; experimental design; bioactivity; encapsulation; nanoparticles
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: http://tede.unicentro.br:8080/jspui/handle/jspui/1591

B. dracunculifolia has been used in popular medicine because of its bioactive properties, which include anti-inflammatory, antiulcerative and hepatoprotective potentials. Similarly, β-glucans show different biological functionalities, such as immunomodulatory activity, anticarcinogenenic, hypoglycemic, among others. Nanoparticles containing Baccharis dracunculifolia extract and (16)-β-D-glucan (lasiodiplodan) were produced and characterized. In the present study, a multivariate approach using a central composite rotational design 2³ was used to define the best extraction conditions of the bioactive compounds from the leaves of Baccharis dracunculifolia, and an experimental design 2³ was used to define the production conditions of the nanoparticles by atomization in a spray dryer. Lasiodiplodan associated with maltodextrin and gum arabic were studied as matrices. The best extraction conditions of bioactive compounds from the leaves of B. dracunculifolia were 95 % methanol (v/v), 20 minutes extraction time at 70 °C. Under optimized conditions, high total phenolic content compounds (72.5 mg GAE/g) and high free radical scavenging activity DPPH (865.6 μmol TE/g) and ABTS (331.11 μmol TE/g) were obtained. In addition, a high ferric reducing power was observed (1558.00 μmol FeSO4.7H2O/g). The extract also showed antimicrobial activity, demonstrating fungicidal action against Candida tropicalis and C. albicans, and bacteriostatic effect against Escherichia coli, Salmonella enterica Typhimurium and Staphylococcus aureus. Analysis by HPLC-DAD revealed an extract rich in phenolic compounds, such as catechin, caffeic acid, p-coumaric acid, ferulic acid, pinocembrin and rutin. The nanoparticles obtained under optimized conditions (1.5 % lasiodiplodan, 5.0 % B. dracunculifolia extract and 10 % maltodextrin) had an average size varying from 230 to 1210 nm and high total phenolic content (3157.9 μg GAE/g). The mathematical model that best represented the release kinetics of the extract contained in the nanoparticles was the Korsmeyer-Peppas model. Diffusional exponent (n) values of 0.64 at pH 7.7 and 1.15 at pH 2.61 indicated particles with a non-Fickian or anomalous transport system and Super Case II transport, respectively. Thermal analysis showed that the particles exhibit high thermal stability (316 ºC) and X-ray diffraction analysis showed a characteristic diffractive profile of an amorphous structure. In the nanoparticles were found the phenolic compounds: caffeic acid; p-coumaric acid and catechin and comprise a new biomaterial with biological potential for applications in different areas.