Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Kovalski, Gláucia
 |
| Orientador(a): |
Rodrigues, Paulo Rogério Pinto
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| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Estadual do Centro-Oeste
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Bioenergia (Mestrado)
|
| Departamento: |
Unicentro::Departamento de Ciências Agrárias e Ambientais
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://tede.unicentro.br:8080/jspui/handle/jspui/1069
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Resumo: |
Biofuels production may occur from various raw materials, for example, from glycerol and waste of paper industry. Glycerol is the main byproduct of biodiesel production, and for being an excellent source of carbon, can be availed by microorganisms to perform anaerobic fermentation and convert it into ethanol. The paper industries biomass waste has the cellulose as a main compost which can be used in fermentation processes as a source of carbon. Therefore, this study aims to produce ethanol from anaerobic fermentation of glycerol and waste from the paper industry by the bacteria Escherichia coli ATCC 25922. For determination of CFU (colony forming units) the plating technique and serial dilution was used. The total cell number was estimated by the method of counting in a Neubauer chamber. Glycerol was characterized by physical-chemical analysis of pH, conductivity, density, appearance and visual color. The residual fiber was characterized by Spectroscopy Infrared Region (IR), elemental composition and SEM (scanning electron microscopy). A multivariate 2² experimental design with a central point was used. The fermentation process was accomplished in a closed system for 72 hours at 36 °C ± 0.5, anaerobically. The fermented concentrate was distilled in a simple distillation apparatus and quantifies the content of ethanol in the crude distillate by UV-VIS method (NBR 13920). The presence of ethanol was confirmed by gas chromatography analysis (GC-FID). Ethanol obtained was characterized by physical-chemical analysis. The statistical analysis was performed using the software Design-Expert®. The results indicated a trend of increased ethanol production using 2 g of cellulosic material and 4 mL of bacteria E. coli. The highest concentration of ethanol obtained were 83 and 81 ° GL. The work showed to be possible to produce ethanol from microbial fermentation of glycerol and cellulosic wastes with low cost in the process, and use of surplus waste in environment. |
