Detalhes bibliográficos
| Ano de defesa: |
2012 |
| Autor(a) principal: |
Beitel, Susan Michelz
 |
| Orientador(a): |
Knob, Adriana
|
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
UNICENTRO - Universidade Estadual do Centro Oeste
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biologia Evolutiva (Mestrado)
|
| Departamento: |
Unicentro::Departamento de Biologia
|
| País: |
BR
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://localhost:8080/tede/handle/tede/386
|
Resumo: |
β-Glucosidases have a great potential to be used in various biotechnological processes, such as in food and feed industries, biomass hydrolysis for bioethanol production and its use to enhance the flavor of wine, tea and fruit juice. This work describes the ?-glucosidase production by Penicillium miczynskii. The effect of pH, time and condition of cultivation, temperature, different carbon sources and concentration of the selected agro industrial waste on extracellular ?-glucosidase production was studied in submerged fermentation. Additionally, the enzyme produced was biochemically characterized. The best conditions for P. miczynskii ?-glucosidase production were 3% pineapple peel as carbon source, under stationary condition, for 9 days, pH 5.5 and temperature of 20°C, achieving to 2,82 U/mL. The enzyme showed best activity on pH 4.5-5.0, at 65°C. It was stable at 55°C and 60°C, with a half-life of 50 and 40 min, respectively. The ?-glucosidase was slight activated in the presence of ?-mercaptoethanol and Ca²+ and strongly inhibited by SDS, Zn²+ and Hg²+. Also, sodium citrate, NH4+ and Ba²+ were able to inhibit this enzyme, especially at 10 mM concentration. The article provides a novel ?-glucosidase which presents favorable industrial properties, such as its production in pineapple waste, an inexpensive and abundant agroindustrial residue, and high hydrolysis activity at elevated temperatures. |
