Avaliação de Parâmetros Associados ao Estresse Oxidativo em Pacientes com Doença de Alzheimer

• Ano de defesa: 2015
• Autor(a) principal: HORST, JACQUELINE APARECIDA EIDAM
• Orientador(a): Bonini, Juliana Sartori
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual do Centro-Oeste
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas (Mestrado / Associação Ampla com UEPG)
• Departamento: Unicentro::Departamento de Farmácia
• País: Brasil
• Palavras-chave em Português: Estresse oxidativo; hemograma; Vitamina C; Glutationa Peroxidase; δ-ALA D; enzimas antioxidantes; Doença de Alzheimer
• Palavras-chave em Inglês: oxidative stress; CBC; vitamin C; glutathione peroxidase; δ-ALA D; antioxidant enzymes; Alzheimer’s disease
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::FARMACIA
• Link de acesso: http://tede.unicentro.br:8080/jspui/handle/jspui/672

The Alzheimer’s disease (AD) is a dementia of chronic and progressive nature that causes disturbance in the cognitive functions. Studies indicate that this pathology can be the result of oxidative stress generated in the organism of these individuals. Thus, the present study had as objective to compare the cognitive function, parameters of oxidative and hematological stress in patients with AD and the control group, registered in the Specialized Component of Pharmaceutical Assistance (CEAF), from Guarapuava municipality. 34 patients and 34 controls participated in the study, paring according to gender, age and, at least, one comorbidity. They received home visits for cognitive and socio-demographic evaluation, biochemical and hematological analyses. The cognitive evaluation was carried out using Mini-mental State Examination (MMSE). To evaluate the oxidative stress level, δ-ALA-D, GPx enzymes and vitamin C were dosed through colorimetric methods according to the methodology. For staging the disease, the scale Clinical Demential Rating was used. The sampling studied was comprised of 61.8% (n=21) of female individuals and 38.2% (n=13) of male individuals and their respective controls, with age average of 78.1 ± 8.9 years and median age equal to 80.0 ± 11.0 years. Concerning the physical activity, when comparing DA patients with the control group, there was a statistical difference, showing that the physical activity reduces as the DA level increases. In relation to the existent comorbities, there was no statistical difference when comparing DA patients and their controls. The cognitive function of most of the patients is reduced in the moderate and severe stages of the disease. As for the correlation between enzymes, when comparing patients with AD and the control group, the activity of the enzymes δ-ALA D and GPx have not showed significant difference in the early stage of the disease (CDR1) when dosed in erythrocytes. In the CDR2 and CDR3 stages, lower values were found in the patients with AD (p<0.001). Vitamin C in the three stages of the disease, presented reduced plasma levels for the AD group compared to the control group (p<0.001). We observed that the hematological parameters showed significant variation in the MCV and MCHC of red blood cells and according to the advance of AD, the pro-antioxidant effect of the enzymes δ-ALA D and GPx, and vitamin C is depleted. These results launch the possibility of following these biochemical and hematological parameters in patients with any kind of cognitive decline, aiming at diagnostic as well as prognostic guidance.