Efeitos de um derivado semissintético do [6]-gingerol na autofagia e apoptose em células de câncer de mama
Ano de defesa: | 2017 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de São Carlos
Câmpus São Carlos |
Programa de Pós-Graduação: |
Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF
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Departamento: |
Não Informado pela instituição
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País: |
Não Informado pela instituição
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Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | https://repositorio.ufscar.br/handle/20.500.14289/10871 |
Resumo: | In this work, we describe a semisynthetic derivative of [6]-gingerol called SSi6, which induces cytotoxic effects on the MDA-MB-231 breast tumor, showing a greater selectivity for these cells when compared to the non-tumor MCF-10A , In addition, the substance was determined to induce autophagy and apoptosis in tumor cells, with less pronounced effects on non-tumor cells. The coexistence of both events (autophagy and apoptosis) was demonstrated by flow cytometry, which evidenced an increase in the percentage of apoptotic cells after 24 hours of treatment, these results were also confirmed by the analysis of the gene and protein expression of caspase-3 using Real-time PCR and western blotting respectively. Induction of autophagy in SSi6-treated cells has been demonstrated through acid vesicle organelle (AVOs) detection assays and the expression of genes encoding the LC3-I and II microtubule-associated proteins to quantify the abundance of autophagosomes in MDA-MB-231 cells. Thus, these results indicate that SSi6 has the potential to induce autophagy and apoptosis, as well as antitumor and antimetastatic properties in MDA-MB-231 breast tumor cells. |