Papel do sistema histaminérgico nos processos de aprendizagem e memória em Carassius auratus após ablação cerebelar

Detalhes bibliográficos
Ano de defesa: 2009
Autor(a) principal: Garção, Diogo Costa
Orientador(a): Mattioli, Rosana lattes
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de São Carlos
Programa de Pós-Graduação: Programa de Pós-Graduação em Fisioterapia - PPGFt
Departamento: Não Informado pela instituição
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/5244
Resumo: This study investigated the effect of post-training treatment with L-histidine, Pyrilamine and Zolantidine on the memory consolidation of inhibitory avoidance in Carassius auratus submitted to cerebellar ablation. Cerebellar ablation was performed 3 days before the experiment. The inhibitory avoidance procedure included one habituation day, one training day (5 trials: T1, T2, T3, T4, T5) and a test day (TE). On the training day, each fish was individually placed in a white compartment separated from a black compartment by a sliding door. After 30s, the door was opened. When the fish crossed into the black compartment, a weight was dropped in front of it (aversive stimulus). An intraperitoneal injection with saline or L-histidine (100 mg/kg), Pyrilamine (35 mg/ml) and Zolantidina (ZO) was administered 10 minutes after the trials. The difference in the time elapsed until the fish entered the black compartment (latency) during training and testing was taken as an indicator of behavioral learning and memory. Data were analyzed by ANOVA and the Student Newman-Keuls test (p< 0.05). The three experiments indicated that in T5, all groups had similar latencies that were significantly higher than T1 latencies, indicate that all groups were able to acquire inhibitory avoidance, suggesting that cerebellar ablation does not impair learning. However, twenty four hours after the trainings, the animals with ablation indicating retention of the task while that the animals of the Sham group do not consolidate memory. The results of experiment I showed that the L-histidine reverted the effect of injury. Experiment II demonstrated that the Pyrilamine does not influence in the consolidation of the memory and the data of experiment III indicated that the zolantidina facilitates the acquisition of inhibitory avoidance independent of the cerebellum. These results suggest that the cerebellum is involved in the memory consolidation of inhibitory avoidance and/or interpretation of the aversive value and that this process is mediated by H2 receptors.