Desenvolvimento de biossensores eletroquímicos baseados em acetilcolinesterase visando identificar extratos e substâncias para o controle de formigas cortadeiras

• Ano de defesa: 2019
• Autor(a) principal: Araújo, Sean dos Santos
• Orientador(a): Fernandes, João Batista
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Carlos; Câmpus São Carlos
• Programa de Pós-Graduação: Programa de Pós-Graduação em Química - PPGQ
• Palavras-chave em Português: Produtos naturais; Acetilcolinesterase; Biossensores
• Palavras-chave em Inglês: Natural products; Acetylcholinesterase; Biosensors
• Área do conhecimento CNPq: CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ORGANICA::QUIMICA DOS PRODUTOS NATURAIS
• Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/14469

Brazil, which is a continental country, has an appropriate climate for various agricultural practices. Insects, in general, are considered pests for agricultural areas, among them, an Atta sexdens rubropilosa (leafcutter ant) that attacks as eucalyptus, pine plantations among others. Control of this pest becomes essential for the best productivity of cultivated areas. This control can be directed to inhibition of enzymes present in the target insect organism. In this line, the present work aims to immobilize the enzyme acetylcholinesterase (AChE) in SPEs (screen printed electrode), using the layer-by-layer technique, using AuNPs-GSH (glutathione-decorated gold nanoparticles) and EDC reagents ( 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide) and HOBt (Hydroxybenzotriazole) which establish the covalent bonds responsible for immobilizing an AchE in the SPEs. Electrochemical responses confirm effective immobilization of the enzyme with this developed protocol. For inhibition assays, Azadiractin limonoid, Toona ciliata crude extract and Picramnia riedelli and P. ciliata crude extracts showed significant inhibition percentage against AChE. The 1H NMR spectra of the crude extracts used were obtained to propose natural compounds present in them and to indicate possible relationships with the results of the enzymatic assays. These extracts showed good insecticidal activity against the leafcutter ant.