Bioprospecção de actinobactérias endofíticas com potencial antimicrobiano isoladas de Theobroma cacao

• Ano de defesa: 2023
• Autor(a) principal: Pinto, Lívia Freitas da
• Orientador(a): Sousa, Cristina Paiva de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Carlos; Câmpus São Carlos
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia - PPGBiotec
• Palavras-chave em Português: Actinobactérias; Metabólitos secundários; Bioatividade; Atividade antimicrobiana
• Palavras-chave em Inglês: Actinobacteria; Secondary metabolites; Bioactivity; Anti-bacterial activity
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::MICROBIOLOGIA
• Link de acesso: https://repositorio.ufscar.br/handle/ufscar/19142

Actinobacteria constitute one of the largest bacterial phyla and are classified as Gram-positive bacteria, being ubiquitous in aquatic and terrestrial ecosystems. They are recognized for their ability to produce secondary metabolites with different functionalities, among which are bioactive compounds with antimicrobial activity. This study bioprospecting and identification of endophytic actinobacteria isolated from Theobroma cacao grown in the region of Paragominas, Pará. region of Paragominas, Pará, Brazil and evaluated the biotechnological potential of these strains in the production of antimicrobial agents. After isolation, it was possible to verify the presence of 11 distinct endophytic bacteria. bacteria. The identification of these isolates, determined by phenotypic analysis and molecular characterization, proved that 4 of the 11 belonged to the actinobacteria group, and these were classified at the level of Microbacterium (3 strains) and Curtobacterium (1 strain). The other isolates were characterized as Pseudomonas (2 strains), Bacillus (1 strain), Ralstonia (1 strain) and Methilobacterium (3 strains). The 4 strains from the Actinobacteria phylum were submitted to the antimicrobial activity evaluation test activity. All of them inhibited the growth of Staphylococcus aureus (inhibition halos diameters ranged from 2.3 to 3 cm), proving to be effective against this Gram-positive bacterium. However, they showed no activity against Escherichia coli.