Avaliação do potencial antimicrobiano e leishmanicida de materiais orgânicos derivados da base de Schiff N,N-bis’(Salicilideno)-1,2-fenilenodiamina

• Ano de defesa: 2017
• Autor(a) principal: Soriano, Bianca
• Orientador(a): Sousa, Cristina Paiva de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Carlos; Câmpus São Carlos
• Programa de Pós-Graduação: Programa de Pós-Graduação em Biotecnologia - PPGBiotec
• Palavras-chave em Português: Microrganismos; Leishmania; Salofen; Ensaios de microdiluição
• Palavras-chave em Inglês: Microorganisms; Microdilution assays.
• Área do conhecimento CNPq: CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA; CIENCIAS BIOLOGICAS::PARASITOLOGIA::PROTOZOOLOGIA DE PARASITOS
• Link de acesso: https://repositorio.ufscar.br/handle/20.500.14289/9507

Bacteria and fungi are the main etiological agents potentially causing health care-related infections and the fourth leading cause of death, according to the WHO. Parasitic agents such as Leishmania infantum chagasi, the causative agent of leishmaniasis, neglected and endemic disease, have treatments that are not always effective and have serious side effects. Resistance to antibiotics, antimycotics and leishmanicides, has increasingly induced bioprospecting and synthesis of new substances with pharmacological properties. The Schiff bases, especially those derived from the N, N’-bis (salylcilidene) -1,2-phenylenediamine linker, also known as Salofen, have been studied in order to demonstrate the physical, chemical and biological properties due to Variety of potential technological applications. The objective of this work was to evaluate the biotechnological potential of the Schiff N, N’-bis (salylcilidene) -1,2-phenylenediamine (Salofen) base derivative in relation to the antimicrobial and leishmanicidal activity. Agar diffusion techniques and microdilution assays were used for bacterial and yeast studies, and the colorimetric method with Alamar Blue® for Leishmania tests at 24 and 48 hours. Inhibition halos were observed for all microorganisms tested, with the highest mean halos (30 mm) for Candida albicans. Dilution assays have shown promising results, with inhibition rates above 90%, emphasizing better Gram-negative efciency. The leishmanicidal effect was also observed with IC50 at 1.06 mg.mL−1 at 24 hours and 0.66 mg.mL−1 at 48 hours. Thus, the data collected suggest that the Schiff base tested has an antimicrobial and leishmanicidal potential of the compound studied, suggesting a biotechnological application.