Efeitos da inflamação no período neonatal sobre o perfil inflamatório e oxidativo na sepse experimental na vida adulta

• Ano de defesa: 2013
• Autor(a) principal: Lunardelli, Adroaldo
• Orientador(a): Donadio, Márcio Vinícius Fagundes
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul
• Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina/Pediatria e Saúde da Criança
• Departamento: Faculdade de Medicina
• País: BR
• Palavras-chave em Português: MEDICINA; PEDIATRIA; CITOCINAS; POLISSACARÍDEOS; SEPSE
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA
• Link de acesso: http://tede2.pucrs.br/tede2/handle/tede/1424

During the neonatal period, the administration of lipopolysaccharide (LPS) generates a stressful immune stimulation resulting in the expression of cytokines in the central nervous system. As result, brain changes in certain regions of the hippocampus of adult rats, as well as activation of the hypothalamic-pituitary-adrenal axis (HPA) in neonates is observed. The objective of this study is to evaluate the long-term effects of neonatal inflammation on the oxidative and inflammatory status during experimental sepsis in adult life. On day ten after birth, Balb/c mice received different treatments: nLPS, received 100 μL of a 100 μg/kg i.p. LPS solution or nSal, received 100 μL of i.p. saline solution. As adults, animals were submitted to i.p. administration of saline solution or LPS. After 12 hours, serum samples and liver were collected to measure inflammatory cytokines and to determine mitochondrial complexes and oxidative stress, respectively. There was an increase in the fear/anxiety behavior in the nLPS group. The complexes II and II-III increased in the nLPS saline group when compared to control. The LPS administration in the adult females induced a decrease of the glutathione enzyme activity. There were no differences in the inflammatory cytokines. The results indicate that the neonatal inflammation was effective in inducing programming. The mitochondrial respiratory chain metabolism seems to be influenced by the neonatal inflammation in males. LPS neonatal stress seems not to change the cytokine inflammatory profile in an experimental model of sepsis