Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Hartmann, Rafael Chies
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| Orientador(a): |
Figueiredo, José Antonio Poli de
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| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
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| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Pontifícia Universidade Católica do Rio Grande do Sul
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| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Odontologia
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| Departamento: |
Faculdade de Odontologia
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| País: |
Brasil
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| Palavras-chave em Português: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://tede2.pucrs.br/tede2/handle/tede/6065
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Resumo: |
Introduction: Knowledge of endodontic microbiology remains the subject of numerous studies. Because of this, the present study aimed to evaluate characteristics related to cell viability, topography and structure of the biofilm formed within the root canal of teeth coupled to an intraoral device. Methods: Lower intraoral devices were made including four roots of human incisors, which have only the cervical third exposed to the oral environment. A volunteer used them for 14 days, three different times. The roots were fragmented and stained with SYTO 9 and propidium iodide and analyzed by Confocal Scanning Laser Microscopy. Viability was determined qualitatively through scores based on the fluorescence of the two image channels, in each third of the root canal. Then, the samples were analyzed in scanning electron microscopy (SEM), in order to verify aspects regarding biofilm structure, topography and density. Qualitative scores determined the biofilm density for each third of the root canal. Statistical analyses were carried out through the Kruskal–Wallis and Dunn post hoc, and Mann-Whitney tests (p< 0.05). Results: Cell viability was observed throughout the length of the root canal, with no statistical difference between the scores of live and dead microorganisms. In SEM, coccus and rods were the most observed bacterial morphologies, being present in almost all samples. However, filaments, spirochetes and human cells were also observed. The density of the biofilm showed a higher concentration in the cervical third and it was statistically different from the other thirds. Conclusions: It was possible to verify that the experimental model presented herein, was efficient in reproducing, in situ, morphotypes of the biofilm of in vivo endodontic primary infections. |
