Associação entre infecção pelos vírus HPV e EBV e neoplasias malignas de boca : análise histomorfométrica

• Ano de defesa: 2015
• Autor(a) principal: Jornada, Vanessa Chidiac
• Orientador(a): Cherubini, Karen
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Faculdade de Odontologia
• País: Brasil
• Palavras-chave em Português: ODONTOLOGIA; ESTOMATOLOGIA CLÍNICA; NEOPLASIAS BUCAIS; CARCINOMA; PAPILLOMAVÍRUS HUMANO
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://tede2.pucrs.br/tede2/handle/tede/6212

Head and neck cancer represents a group of diseases affecting the face and upper aerodigestive tract. Early diagnosis of these neoplasms and their etiologic factors is crucial for patient’s survival and treatment. In the last decades, cause-and-effect relationship between some viruses and malignancies has been widely accepted. Recently, the role of Human papillomavirus (HPV) and Epstein-Barr virus (EBV) has been shown in the pathogenesis of oropharyngeal carcinoma, undifferentiated nasopharyngeal carcinoma and lymphomas. Nevertheless, the role of such viral agents in oral squamous cell carcinoma (OSCC) is still controversial. This study aimed to investigate HPV, EBV, p53 and Ki-67 in OSCC and oral lymphoma, considering clinical and histological features. The sample was composed of archived material (medical records and paraffin blocks of biopsied specimens) from patients with OSCC, oral lymphoma, and oral traumatic fibrous hyperplasia. The biopsied specimens and medical records were allocated into five groups: (1) 16 samples from patients with OSCC grade I (well-differentiated); (2) 16 samples from patients with OSCC grade II (moderately-differentiated); (3) 19 samples from patients with OSCC grade III (poorly-differentiated); (4) 14 samples from patients with oral non-Hodgkin lymphoma; and (5) 19 samples from patients with oral traumatic fibrous hyperplasia. Biopsied specimens were analyzed for HPV, EBV, p53 and Ki-67 expression by immunohistochemistry, and clinical data concerning age and gender of the patients, size and duration of the lesion as well as habits and comorbidities were collected. Marker expression was evaluated according to clinical features and histological grade of the tumors. There was no association of HPV and EBV with the tumors analyzed. Ki-67 expression was significantly lower in the fibrous hyperplasia group with no other significant differences. p53 expression was significantly lower in the lymphoma and fibrous hyperplasia groups than the OSCC groups, with no other significant differences. OSCC grade II and III were associated with male gender and tobacco smoking, while grade III was also associated with alcohol consumption. There was no association of the tumors with either chimarrão or comorbidities. Duration of the lesion was inversely correlated to lesion size, which in turn was inversely correlated to HPV. EBV and HPV were positively correlated to each other, as was Ki-67 to lesion size and p53, which was correlated to HPV.