Avaliação do biomaterial de PLGA com hormônio de crescimento recombinante humano (rhGH) em modelo animal

• Ano de defesa: 2017
• Autor(a) principal: Garcia, Ricardo Fernandes
• Orientador(a): Pagnoncelli, Rogério Miranda
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul
• Programa de Pós-Graduação: Programa de Pós-Graduação em Odontologia
• Departamento: Faculdade de Odontologia
• País: Brasil
• Palavras-chave em Português: Hormônio do Crescimento; Remodelação Óssea; PLGA; Enxerto Autógeno
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://tede2.pucrs.br/tede2/handle/tede/7370

More and more studies are focusing on the combination of matrices that have osteoconductive characteristics with osteoinductive proteins. These proteins can stimulate the differentiation of mesenchymal and osteoprogenitor cells into osteoblasts and thereby increase the migration of cells related to bone formation within the defect site. The main materials used for these purposes are biodegradable polymers, such as PLA (poly lactic acid) and PLGA (poly lactic glycolic acid). Several drugs are used to be released in these systems, such as antibiotics, contraceptives and proteins, including human growth hormone (GH). RhGH in bone tissue promotes the increased deposition of proteins by chondrocytes and osteoblasts, an increase in the number of mitoses and the conversion of chondrocytes into osteoblasts. Thus, it is necessary to evaluate this biomaterial (PLGA \ rhGH) as a controlled release device. Thus achieving the presence of rhGH in the site of bone healing. For this study was used as animal model, wistar rats. The study was carried out in accordance with Law No. 11,794 of October 8, 2008, as well as following the Brazilian Directive of Practice for the Care and Use of Animals for Scientific and Educational Purposes - DBPA of CONCEA. Project approved by CEUA of PUCRS under number 15/00461. Thirty adult wistar rats were used, in which they were submitted to bone defects with Carbide spherical drill number 704, the defect corresponded to the diameter of the drill. The rats were submitted to the same treatment but suffered euthanasia at different times (07, 15 and 20 days). The femurs of the rats were radiographed in the range of 7, 15, and 20 days to gauge the optical density. Slides were then produced for histological analysis, with cuts of approximately 5 μm thick and stained with hematoxylin / eosin (HE). Three more representative cuts of each blade were chosen. The systemic repercussion of rhgh was assessed through IGF-1 levels through blood collection that was performed before each euthanasia, in the control group and in the experimental group. The blood collected was centrifuged (2500 revolutions per minute for 10 min) and serum obtained stored in a freezer at -20 ° C for further determination of IGF-1 plasma levels using the method based on an enzymatically amplified assay of the " Sandwich, "held at the Senhor dos Passos laboratory in Porto Alegre, RS. For statistical analysis, the Tukey test was applied, 5% significance level and ANOVA variance analysis. There were no statistically significant differences in bone mineral densities, however numerically the PLGA\RhGH grafts always had a greater amount of shades of gray. In conclusion, defects with PLGA\RhGH grafts showed a higher optical density. A higher mineral density compared to the autogenous graft In the histological analyzes there was also no statistically significant difference between the autogenous and PLGA\RhGH grafts. However, by numerical analysis we observed a better and equal performance between the PLGA\RhGH graft and autogenous graft. Defects with PLGA\RhGH grafting probably had faster healing and maturation than autogenous grafts.