Detalhes bibliográficos
| Ano de defesa: |
2012 |
| Autor(a) principal: |
Cogo, Deborah Meirelles
 |
| Orientador(a): |
Pelisser, Fabiana Vieira Vier
|
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Pontifícia Universidade Católica do Rio Grande do Sul
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Odontologia
|
| Departamento: |
Faculdade de Odontologia
|
| País: |
BR
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://tede2.pucrs.br/tede2/handle/tede/1168
|
Resumo: |
Introduction: The aim of this in vitro study was to evaluate the effect of calcium hydroxide (CH), omeprazole (O) and associations of these substances against Enterococcus faecalis, as well as to evaluate if the acid-catalysation of the O had any influence in the results. Methods: The methodology used was adapted from the CLSI (Clinical Laboratory and Standards Institute) macrodilution MIC (Minimum Inhibitory Concentration) test. Antibacterial activity was evaluated against Enterococcus faecalis (ATCC 29212). Solutions with different concentrations of CH, associated or not with O were prepared and placed in contact with the inocula in the culture medium Mueller Hinton. The tubes remained in a dry-heat oven for 24h, serial dilutions were cultivated in agar plates, and after 48h, colony-forming units (CFU) mL-1 was determined. The MIC was also evaluated. Data were statistically analyzed using ANOVA test with Tukey posthoc, with a level of significance of 5%. Results: The MIC for CH was 32 mg mL-1, however reduced to 16 mg mL-1 when associated with O. The O and AO had similar behavior. Conclusion: O potentiates the effect of CH, since the association of these drugs reduces the MIC for E. faecalis. The acidification of O, when associated with CH in different concentrations, did not influence its effect. |
