Avaliação de células-tronco mesenquimais murinas órgão-específicas quanto à capacidade de diferenciação in vitro em células produtoras de insulina

• Ano de defesa: 2010
• Autor(a) principal: Sesterheim, Patrícia
• Orientador(a): Antonello, Ivan Carlos Ferreira
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul; Porto Alegre
• Palavras-chave em Português: MEDICINA; NEFROLOGIA; DIABETES MELLITUS; CÉLULAS-TRONCO MESENQUIMAIS; EXPERIMENTAÇÃO ANIMAL; INSULINA
• Link de acesso: http://hdl.handle.net/10923/4382

Type I diabetes mellitus (DM1) is an organ-specific autoimmune syndrome characterized by the selective destruction of insulin-producing β cells in the pancreatic islets. The search for therapeutic alternatives for DM1, the β cell mass and consequently the reconstitution of physiological secretion of insulin have been extensively done. Among different treatments strategies studied, cell therapy based on mesenchymal stem cells is one of the most extensively studied. Searching for a product that mimics qualitative and quantitatively the characteristics of pancreatic β cells, protocols must be improved and the capacity of (trans) differentiation of new sources of tissue-specific must be explored. In this way, the aim of this work was to compare the capacity of expansion and differentiation in vitro of murine mesenchymal stem cells, isolated from kidney, pancreas and bone marrow, evaluating its capacity of differentiation into insulin-producing cells (IPCs). From the results, it was evident that these cell populations were able to (trans) differentiate into the pancreatic endocrine cell-like phenotype. When cultured in vitro in a rich inducing-media, cells were characerized by cluster formation with spherical morphology, positive dithizone staining and expression of insulin-1 at the mRNA and protein level. Moreover, IPCs derived from mesenchymal stem cells reversed the pancreatic hyperglycemic state when transplanted into the kidney capsule of chemically induced diabetic mice, indicating that in vitro they are able to differentiate into functional IPCs.