Polimorfismo da apolipoproteína E e perfil de distribuição de subfrações de lipoproteínas

• Ano de defesa: 2004
• Autor(a) principal: Costa, Patrícia de Moraes
• Orientador(a): Vieira, José Luiz da Costa
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Pontifícia Universidade Católica do Rio Grande do Sul; Porto Alegre
• Palavras-chave em Português: MEDICINA; APOLIPOPROTEÍNAS; POLIMORFISMO GENÉTICO HUMANO; HIPERLIPIDEMIA; GERIATRIA; ARTERIOSCLEROSE CORONÁRIANA; DOENÇAS CARDIOVASCULARES
• Link de acesso: http://hdl.handle.net/10923/4406

Background – Cardiovascular diseases are the fifth cause of death all over the world and projections show that they will be the first cause in the year 2020 if preventive measures are not provided. Dislipidemia is among the main risk factors to coronary disease, with it’s participation intensity as a risk factor depending on environmental components and genetic characteristics of each individual. Among the genetic factors that influence atherosclerosis, apolipoprotein E is the most studied one and is associated with changes in lipoprotein levels depending of the respective allele envolved. With the recent advances in the lipid metabolism study, researches have been started regarding the roll of lipoproteins subfractions and the genetic influence, specially the apolipoprotein E polymorphism, in the development of atherosclerosis. Objective – To correlate the apolipoprotein E polymorphism and the lipoprotein subfraction distribution profile of dislipidemic individuals. Population and methods – Cross-sectional, descriptive and analytical study. Thirty-five individuals with dislipidemia were analyzed. Their ages were between fifty and seventy-nine years-old. The data were obtained through structured interview and laboratorial evaluation. Lipid profile were evaluated using enzymatic method and lipoproteins subfractions profile were evaluated by Nuclear Magnetic Ressonance spectroscopy method and apolipoprotein E polymorphism using Polymerase Chain Reaction - Randon Fragment Lengh Polymorphism technique. Results – The genotypic frequencies found were symilar to the pattern found in the adult caucasian population in general, with predominance of the 3 3 genotype. The lipid profile and the lipoprotein subfractions showed a normal pattern of distribution, except for the VLDL size. Analyzing this varieties according to the apolipoprotein E group (group three: 2 3 and 3 3, group four: 3 4 and 4 4 ), statistically significant differences were not found to any of the variables. Conclusion – In the present study with dislipidemic patients we did not find association between the apolipoprotein E polymorphism and the lipoprotein subfraction distribution profile .