Detalhes bibliográficos
| Ano de defesa: |
2009 |
| Autor(a) principal: |
Marinho, Regina Helena
 |
| Orientador(a): |
Silva, Carlos Alberto |
| Banca de defesa: |
Pontako, Fernanda
,
Silva J??nior, Jos?? Ant??nio |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Nove de Julho
|
| Programa de Pós-Graduação: |
Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
|
| Departamento: |
Sa??de
|
| País: |
BR
|
| Palavras-chave em Português: |
|
| Palavras-chave em Inglês: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://bibliotecatede.uninove.br/tede/handle/tede/816
|
Resumo: |
Objectives: To analyze the levels of degradation of extracellular matrix (ECM) in the urine in patients with COPD and their correlation with pulmonary function. Methods: patients with COPD (n = 20) and healthy controls (n = 10) were selected and submitted the pulmonary function testing by spirometry. The degradation of ECM was assessed in urine samples in the group with COPD and control of two strategies: determination of hydroxyproline, to evaluate collagen degradation, and detection and determination of the concentration of elastin fragments by ELISA using anti-elastin. Results: The difference between the concentration levels of hydroxyproline in urine of patients with COPD was not different compared to the control group. However, it was observed that the concentration of elastin fragments in the urine of patients with COPD was highly significant (p=0.002) and correlated negatively with FEV1 parameter of pulmonary function tests. Conclusion: This results open perspectives for the characterization of degradation products of ECM by mass spectrometry (MALDI-TOF/LC-MS/MS), a technology highly specific and sensitive for the determination of amino acid sequences of peptides of interest. |
