Efeito da fotobioestimula????o no edema e hiperalgesia induzido pelo veneno Bothrops moojeni e por duas mitoxinas isoladas deste veneno

• Ano de defesa: 2014
• Autor(a) principal: Andrade, Nikele Nadur
• Orientador(a): Zamuner, Stella Regina
• Banca de defesa: Costa, Maricilia Silva , Marcos, Rodrigo Labat , Carvalho, Paulo de Tarso Camillo de , Vessoni, Sandra Coccuzzo Sampaio
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Nove de Julho
• Programa de Pós-Graduação: Programa de P??s-Gradua????o em Ci??ncias da Reabilita????o
• Departamento: Sa??de
• País: BR
• Palavras-chave em Português: bothrops moojeni; miotoxina; dor inflamat??ria; edema; laser de baixa pot??ncia; LED
• Palavras-chave em Inglês: bothrops moojeni; myotoxin; inflammatory pain; edema; low level laser; LED
• Área do conhecimento CNPq: CIENCIAS DA SAUDE
• Link de acesso: http://bibliotecatede.uninove.br/tede/handle/tede/906

Envenoming induced by Bothrops snakes is characterized by drastic local tissue damage involving hemorrhage, myonecrosis and proeminent inflammatory and hyperalgesic response. The most effective treatment is antivenom therapy, which is ineffective in neutralizing the local response. Herein, it was evaluated the effectiveness of light-emitting diode (LED) at wavelengths of 635 and 945 nm in reducing inflammatory hyperalgesia induced by Bothrops moojeni venom (BmV) or myotoxins (MjTX-I and -II) isolated from this venom. LBP was irradiated at a wavelength of 685 nm and 2.2 J/cm2 energy density, red LED in wavelength of 635 nm and 4 J/cm2 energy density and infrared LED in 945 nm wavelength and energy 4 J/cm2 density. The effect of LBP and LED were evaluated when applied alone or in combination with the antivenom. Our results demonstrated that both LBP as well as LED reduced the hiperalgesic effect induced by the B. moojeni venom alone or in combination with the antivenon treatment. We note that the LBP was effective in reducing levels of mRNA transcript that translates the pro-inflammatory cytokines IL-1 ??, IL-6, and TNF-??, as well as receptors for bradykinin B1 and B2. Still, the LBP was able to increase the expression of anti-inflammatory cytokine IL-10, on muscle plant of mice. Treatment with LBP and LED reduced edema formation induced by MjTX MjTX-I and-II in a similar manner. The combination of antivenom with the LED did not improve the effect of low-level therapy alone, in reducing the edema formation induced by MjTX-I or II. The results obtained in this study have shown that LBP and LED in the parameters used can be an alternative for the treatment of the local effects caused by bothropic venom accidents as well as an important tool for studying the mechanisms involved in the inflammatory process induced by poisons or toxins.