Biocompatibilidade de matrizes de colágeno aniônico e sua influência na orientação do crescimento celular

• Ano de defesa: 2004
• Autor(a) principal: Buchaim, Rogério Leone
• Orientador(a): Goissis, Gilberto
• Banca de defesa: Andreo, Jesus Carlos , Chueire, Alceu Gomes , Oliveira, José Américo de , Lucia, Mariângela Borghi Ingraci de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Faculdade de Medicina de São José do Rio Preto
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências da Saúde
• Departamento: Medicina Interna; Medicina e Ciências Correlatas
• País: BR
• Palavras-chave em Português: Rato; Osso; Tíbia; Pericárdio; Tendão; Teste de Materiais; Colágeno; Crescimento Celular; Ratos; Osso e Ossos; Tendões
• Palavras-chave em Inglês: Rat; Bone; Tibia; Pericardium; Tendon; Materials Testing; Collagen; Cell Enlargement; Rats; Bone and Bones; Tendons
• Palavras-chave em Espanhol: Ensayo de Materiales; Aumento de la Célula; Ratas; Huesos; Tendones
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::ORTOPEDIA
• Link de acesso: http://bdtd.famerp.br/handle/tede/228

The present study was aimed at examining the biocompatibility of anionic collagen matrices, their local bone response following implantation in surgically-created bone defects and also the influence of the collagen fiber orientation in the neoformed bone tissue. Seventy two rats ( Rattus norvegicus albinus ) were used in this experiment. The animals were divided into four experimental groups: Group 1 (control), without implants; Group 2, pericardium medullar implants, 12 hours; Group 3, medullar implants of tendon, 24 hours, with the bigger axes of collagen fibers parallel to the bigger axes of the tibia; and Group 4, medullar implants of tendon, 24 hours, with the bigger axes of collagen fibers perpendicular to the bigger axes of the tibia. After the experimental surgery, the evolution of the repair process was microscopically evaluated in 7, 15 and 30 days post-surgery. The results demonstrated the implanted matrices to be biocompatible and to function as a scaffold inducing the formation of bone, mainly in Group 4. At first, the cellularity follows the arrangement of collagen fibers, obtaining a growing multidirectional arrangement, suggesting that the direction and the orientation are related to the direction and to the magnitude of the stress applied to the bone.