Detecção do vírus dengue pela técnica de aglutinação do látex modelo experimental

• Ano de defesa: 2007
• Autor(a) principal: Luppino, Plinio Luis
• Orientador(a): Lima, édimo Garcia de
• Banca de defesa: Cury, Patricia Maluf , Maia, Irineu Luiz , Durigon, Edison Luiz , Vieira, Rubens Rocha
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Faculdade de Medicina de São José do Rio Preto
• Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências da Saúde::123123::600
• Departamento: Medicina Interna; Medicina e Ciências Correlatas::123123::600
• País: BR
• Palavras-chave em Português: Virologia; Vírus da Dengue; Diagnóstico Precoce; Testes de Fixação da Látex; Latex Fixation Tests
• Palavras-chave em Inglês: Dengue Viruses; Early Diagnosis; Latex Agglutination; Viroly; Dengue Virus
• Palavras-chave em Espanhol: Diagnostico Precoz; Pruebas de Fijac?on de Latex
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::MEDICINA::ANATOMIA PATOLOGICA E PATOLOGIA CLINICA::123123::600
• Link de acesso: http://bdtd.famerp.br/handle/tede/21

Dengue is the arthropod-borne transmitted viral disease of highest worldwide prevalence in mortality and morbidity. The proportion is pandemic ranging 1.6 million of infected patients yearly. Clinical presentation associated to epidemiological factors such as dengue prevalence in the patient s origin have been the only mean for early diagnosis. Laboratorial diagnosis, the conclusive, requires several days when there is viral isolation. Serological methods depend on high level of specific antibodies, and molecular methods are not available for the majority of laboratories of diagnosis and routine. The purpose of this study was to develop an agglutination method using latex to detect dengue virus, using biological samples of mice infected with dengue 1 Mochizuki strain by intracerebral via, and anti-dengue 1 specific antibodies from immunized mice. According to the results, this method was feasible for the dengue viruses diagnosis in positive samples of experimental animals. It provides further approaches for rapid detection of dengue in susceptible populations during the first days of the disease.