Salvia officinalis: fracionamento bioguiado de extrato em diclorometano frente a bactérias causadoras de cárie e estudos analíticos em CLAE-DAD

• Ano de defesa: 2013
• Autor(a) principal: Moreira, Monique Rodrigues
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Mestrado em Ciências; UNIFRAN
• Palavras-chave em Português: Salvia officinalis; Manool; Cárie; Método-analítico; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/787

Belonging to the family Lamiaceae, Salvia (Salvia officinalis) is an herb cultivated worldwide due to its culinary seasoning use. Its botanical name is based on its medicinal importance. In latin, “Salvia” means “to cure” and “officinalis” means “medicinal”. Among the several biological activities described for this specie, its antimicrobial potential can be highlighted due at the fact have been broadly investigated and confirmed against a large number of microoganisms. Aiming to explore such potential, this works describes: the obtaining and the bioguided fractioning of a dichloromethane crude extract of Salvia officinalis (SOD) through a panel of carigenic bacteria (Streptococcus mutans, ATCC 25275; Streptococcus sanguinis, ATCC 10556; Enterococcus faecalis, ATCC 4082; Streptococcus sobrinus, ATCC 33478; Streptococus. mitis ATCC 49456; Lactobacillus casei ATCC 11578; Streptococcus. Salivarius, ATCC 25975).Fraction more active (SODH2) presented values of the MIC between 15.68 – 31.36 µg/mL and your secondary metabolite majoritarian (manool) presented values of the MIC of the 6.24 – 24.96 µg/mL. SODH2 and manool required 12 and 6 hours respectively, to compounds exert effects bactericide. It’s theses values denote the potential of Salvia officinalis in the search for new active principles anticariogenic. It is also described the development and validation of an HPLC-DAD analytical method to determine the manool content in SODH2. Moreover, the establishment of a validated method to quantify manool in SODH2 attended to the increasing demands in quality and safety of phytopharmaceuticals.