Alterações proteicas na epiderme humana reconstruída tratada com bioéster derivado de Chenopodium quinoa

• Ano de defesa: 2020
• Autor(a) principal: Andrade, Amanda Caroline Camillo de
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Biotecnologia Industrial; UP
• Palavras-chave em Português: Biotecnologia; Proteômica; Epiderme; Espectrometria de massas; Chenopodium quinoa; CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2298

In vitro models are considered alternative models to animal tests and, therefore, of great importance for applied research for the evaluation of cosmetics, allowing to mimic as tissue conditions and processes in vivo when compared to exogenous agents, such as cosmetic ingredients. The Bioester generated from the Chenopodium quinoa seed is an ingredient of natural origin, rich in essential fatty acids, minerals, amino acids, and antioxidants, conferring highly emollient, moisturizing and restorative properties to the skin. Thus, the present dissertation contemplates a review article that aims to present the types of cells used for cosmetic tests and artificial skin development and an experimental article in which protein alterations of the reconstructed human epidermis (RHE) were analyzed when treated with Quinoa Bioester compared to untreated. For this, the RHE was exposed to the Quinoa Bioester at different times (0h, 3h, 6h, 12h, 24h and 48h) and the triptych digest was analyzed by nanochromatography coupled with mass spectrometry. For data analysis, the software PatternLab for proteomics was used, checking the differentially abundant proteins in the studied conditions. The results demonstrated proteins with increased protein abundance, compared to the culture of untreated 3D cells, in the same time intervals when submitted to the Quinoa Bioester, suggesting the beneficial action when applied to 3D culture. Differentially abundant proteins in the treated conditions Glutamine Synthase, Acyl-Coa, Actin-related protein 2/3 subunits 2 and 4, Cellular Retinoic AcidII and s100-A2 binding protein, are related to the prevention of premature aging, homeostasis, regeneration, protection against ultraviolet radiation and oxidative damage of the skin.