Associação entre BMP2, BMP4, SMAD6 e RUNX2 e lesões periapicais persistentes

• Ano de defesa: 2020
• Autor(a) principal: Hannegraf, Natascha Douat
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Instituto Brasileiro de Informação em Ciência e Tecnologia; Brasil; Departamento 1; PPG1; IBICT
• Palavras-chave em Português: Genética; Lesão Periapical Persistente; Polimorfismo Genético; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2126

Endodontic treatment is a therapy performed when there is colonization of the root canal system by microorganisms and consequent inflammation. The failure of this therapy may occur due to the interaction of several factors, including patient’s genetic factors. The aim of this study is to evaluate whether the presence of genetic polymorphisms in BMP2, BMP4, SDMAD6 and RUNX2 are associated with persistent periapical lesion. A sample of patients was recruited, who underwent endodontic treatment of necrotic tooth in at least one permanent tooth with periapical lesion. At least one year after completion of the treatment, the initial and follow-up radiographs were compared and evaluated for regression, maintenance or increase of the lesion size. Saliva samples were collected from these patients as a source of genomic DNA for real-time PCR test of the single nucleotide polymorphisms (SNP) in BMP2 (rs1005464 and rs235768), BMP4 (rs17563), SMAD6 (rs2119261 and rs3934908) e RUNX2 (rs1200425 and rs59983488). Then, odds ratios and the chi-square test or Fisher's exact test were performed, which did not allow the association of genes with periapical healing. In addition, the samples were subsequently subjected to multifactorial dimensionality reduction analysis, which allowed the association of SNPs of the combination BMP2 with RUNX2 and combination BMP4 with SMAD6 with the periapical bone healing. There was no association between genotype and allele distribution and persistent apical periodontitis (p>0,05). The MDR determined that the polymorphisms rs235768 (BMP2) and rs59983488 (RUNX2) model of SNP-SNP interaction (consistency CV = 10/10; TBC = 0,584; p = 0,026). In conclusion, in this study the combinations of the polymorphisms rs235768 (BMP2) with rs59983488 (RUNX2) and rs17563 (BMP4) with rs2119261 (SMAD6) were associated with a higher risk of persistent apical periodontitis