Impacto da deficiência de estrógeno e da hipofunção e hiperfunção oclusal na expressão gênica de MMP1 na região odontogênica: um estudo em modelo murino

• Ano de defesa: 2020
• Autor(a) principal: Judachesci, Cláudia Salete
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Positivo; Brasil; Pós-Graduação; Programa de Pós-Graduação em Odontologia Clínica; UP
• Palavras-chave em Português: Odontologia; Estrogênios; Osteogênese; Metaloproteinases da matriz; Saco Dentário; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/2244

Increasing evidence shows that estrogen can regulate matrix metalloproteinase activities. However, the interaction of estrogen deficiency and occlusal condition in the expression of matrix-1 metalloproteinases (MMP1) in dental tissues has not yet been elucidated. Thus, the aim of the present study is to evaluate the influence of estrogen deficiency and hypo- and occlusal hyperfunction on the gene expression of MMP1 in the odontogenic region of teeth in continuous growth. Specimens from a previous study in an experimental murine model of estrogen deficiency were used to identify the relative gene expression of MMP1 in the odontogenic region of lower central incisors. In this scenario, rats of the Wistar Hannover lineage, in the prepubertal period, were divided into two groups according to the intervention received: OVX Group - ovariectomy surgery and SHAM Group - fictitious surgery. Hypo- and hyperfunction conditions were established throughout the pubertal period for analysis of MMP1 gene expression among the OVX group and the SHAM group. At the end of the experiment, the animals were euthanized and the hemimandibles were removed. Condylar sections, coronoid process, and anterior region of the hemimandibule were performed to show only the odontogenic region. The evaluation of the relative gene expression of MMP1 was performed using the quantitative real-time PCR technique. The data were evaluated statistically using the GraphPad Prism 8.0 software. Student's t-test was used to compare the means between the OVX and SHAM groups. The level of significance adopted was 5%. There was no statistically significant difference between the OVX and SHAM groups (p=0.33). The conditions of hypo- and occlusal hyperfunction also showed no significant difference (p> 0.05). It can be concluded that estrogen deficiency and the condition of hypo- and occlusal hyperfunction does not influence the expression of MMP1 in the odontogenic region.