Estudo experimental sobre o efeito da tendinite induzida no músculo gastrocnêmio: histopatologia e espectroscopia Raman

• Ano de defesa: 2006
• Autor(a) principal: Lemos, Márcia Maia
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Mestrado em Promoção de Saúde; UNIFRAN
• Palavras-chave em Português: Espectroscopia Raman; Histopatologia; Músculo Esquelético; Tendinite; CNPQ::CIENCIAS DA SAUDE::FISIOTERAPIA E TERAPIA OCUPACIONAL
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/653

Understanding tendinitis physiopathological mechanisms is important for the improvement of therapeutics conducts, prevention and vigilance in health for vulnerable workers and sportists. The aim of present study was to find out the morphological and biochemistry alterations in a collagenase-induced gastrocnemy muscle rat model, using histopathological analysis, Raman spectroscopy of low resolution spectra (785nm) and chemometrics. Forty-eight Wistar male rats, from Universidade de Franca biotery, with an average weight of 180g, were randomly divided into six groups of eight animals each. In five groups, tendinitis was induced by injecting 30 µl of collagenase into the right calcaneous tendon and 30 µl of phosphate-buffered saline solution into the left calcaneous tendon. In the control group, 30 µl of phosphate-buffered saline solution was injected and in the other into the left tendon and the right tendon was preserved. Each group of animals was sacrified on the 1th, 3th, 7th, 14th and 28th after the induced tendinitis; gastrocnemy muscles were removed and submitted to Raman spectroscopy. Afterwards muscles were prepared for histopathological analysis, stained with hematoxiline-eosin. The results shown no statistic significance of the muscle fibers size, however histopathology 3th and 7th, revealed the presence of inflammatory cells and enlargement of the interstitial compartment, compatible with edema. Raman Spectra patterns and chemometrics along the time of the experiment allowed concluding that those techniques are efficient to detect biochemical alteration during the inflammatory process, with potential application for studies of critical phase tendinitis.