Ser mãe de prematuro exige um pouco mais: relatos de experiências

• Ano de defesa: 2017
• Autor(a) principal: Souza, Valdomiro Jose de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade de Franca; Brasil; Pós-Graduação; Programa de Doutorado em Promoção de Saúde; UNIFRAN
• Palavras-chave em Português: Promoção de saúde; Prematuros; Apoio social; Espiritualidade; CNPQ::CIENCIAS DA SAUDE
• Link de acesso: https://repositorio.cruzeirodosul.edu.br/handle/123456789/386

Phytotherapy is commonly used in modern medicine as a therapeutic option alone or in combination with synthetic drugs. Thus, the present study aimed to evaluate the healing and toxicological effects of topical formulations containing 1%, 3% and 10% Copaifera duckei Dwyer oil-resin, applied daily for 14 days, on skin lesions induced on the dorsal dorsal. Groups treated with healing cream and commercial antibiotic ointment were also included. Daily, by direct inspection, the immediate effect of therapies on the skin and perilesional region was verified. On the 4th, 8th and 14th days after treatment, the presence of edema, secretion, skin hyperplasia, scabs and general condition of the wound were classified into scores. To evaluate the percentages of lesion reduction, when compared to the initial lesion, they were photographed on the 4th, 8th and 14th days of treatment and measured automatically in the ImageJ® program. Histologically, by eosin-hematoxylin staining, angiogenesis intensity scores, ulceration, re-epithelialization, inflammatory infiltrate, hemorrhage and congestion were evaluated and, by Gomori's Trichrome, collagen fiber degeneration. Additionally, by the Picrossirus staining, the percentage of collagen fibers was analyzed, using the ImageJ® program and by immunohistochemistry, the macrophage migration inhibiting factor (MIF), type I collagen and type III collagen. Genotoxicity and cytotoxicity were assessed using the bone marrow micronucleus test and hepatotoxicity, by serum levels of liver enzymes (alanine aminotransferase-ALT and aspartate aminotransferase-AST). The results showed that no rodent showed clinical signs that suggested immediate discomfort to dermal treatments. Regarding edema and skin hyperplasia, the animals treated with the formulation containing 10% C. duckei oil-resin were less affected when compared with other treatments, showing the anti-inflammatory effect of the oil. As for the secretion, scabs and general condition of the wounds, at all times of analysis, the oil-resin formulations of C. duckei did not statistically reduce these symptoms, as well as the treatments with conventional drugs. With regard to the percentage of reduction in the areas of lesions, rodents treated with topical formulations at 1%, 3% and 10%, showed a significant reduction when confronted with those not treated, suggesting the healing effect of the C oil-resin. duckei. Regarding the histopathological results of angiogenesis and re-epithelialization, animals treated with the topical oil-resin formulation in the highest concentration showed a significant increase when compared to untreated animals, which probably accelerated skin healing; on the other hand, changes were not noticed in relation to ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration and percentage of collagen fibers. By immunohistochemistry, it was observed that there was no statistical difference between those treated with formulations containing C. duckei oil-resin and those not treated, regarding the number of cells marked by anti-MIF, density of the area marked by anti-MIF. collagen I and III. In the toxicogenetic analysis, no statistical differences were observed in the frequencies of micronuclei and in the ratio of polychromatic erythrocytes to the total number of erythrocytes between the treated animals and those of the negative control, showing the absence of genotoxicity and cytotoxicity, respectively. There were also no statistical differences in relation to hepatoxicity analyzes. In view of the established methodology and the data obtained, it can be admitted that topical formulations of C. duckei oil-resin benefited stages of skin healing, similar to conventional drugs, being promising options in the treatment of superficial wounds and, in addition, not demonstrated genotoxicity and hepatotoxicity.