O efeito terapêutico do composto Bioativo 6-shogaol em modelos animais expostos à ativação imune materna por lipopolissacarídeo: estudo pré-clínico

• Ano de defesa: 2019
• Autor(a) principal: Rosa, Naiana da
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Lipopolissacarídeos; Comportamento; Memória; Fator neurotrófico derivado do encéfalo; 6-shogaol; Mieloperoxidase
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/3148

Objective: To study the effect of 6-shogaol, a bioactive ginger compound with neuroprotective, anti-inflammatory and antioxidant properties, on behavioral and biochemical parameters in a preclinical model based on prenatal bacterial infection from maternal immune activation by lipopolysaccharide (LPS). Methodology: Twelve pregnant Wistar rats received 100 µg/kg of LPS or saline on gestational day (GD) 9.5. Male offspring participated in the study, on the postnatal days (PND) 30 and 55 respectively, they were supplemented with 6-shogaol or saline by gavage (10mg/kg/day) for 5 days. In PND 35 and 60 the animals performed behavioral tests: grooming, crossing, rearing and memory. In PND 37 and 62, death followed by hippocampal dissection for biochemical analyzes of extracellular signal-regulated kinase 1/2 (ERK 1/2), brain-derived neurotrophic factor (BDNF), enolase (NSE), lipid and protein damage and myeloperoxidase (MPO) activity. Result: Prenatal exposure to LPS significantly increased grooming and crossing episodes in PND 37 and 62, reduced rearing episodes in PND 37. Short-term memory (STM) at PND 37 and long-term memory (LTM) at PND 62 worsened. Decreased ERK and BDNF levels in PND 37 and 62, increased levels of NSE and lipid damage in PND 37 and protein damage in PND 62. Administration of 6-shogaol reversed behavioral parameters, improved STM and LTM, in PND 37 and 62. Increased ERK and BDNF levels in PND 37 and 62, reduced lipid damage in PND 37 and MPO activity in PND 62. Conclusion: 6-shogaol reversed behavioral and biochemical parameters in male offspring exposed to prenatal LPS, acting as a therapeutic component against neurological disorders associated with maternal immune activation in an animal model of autism.