As consequências da ativação imune sistêmica sobre o sistema nervoso central após tolerância imunológica no período neonatal: um estudo pré-clínico

• Ano de defesa: 2020
• Autor(a) principal: Gessler, Karen Beatriz
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Tolerância imunológica; Sistema nervoso central; Sistema imune
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/3149

Introduction: Immune activation is characterized by an inflammatory process caused by infectious pathogens, as well endotoxins such as LPS. Although, in childhood, the innate immune system is still developing, it has greater plastic capacity, because external stimuli such as pathogens, may be able to program immune responses until adulthood. The investigation of the tolerance mechanism, as a consequence of exposure to LPS in low doses in the neonatal period, becomes relevant, as it aims to understand whether or not this first immune activation may predispose to the development of late changes after a second exposure to endotoxemic as an adult. Objective: Evaluate the consequences of systemic immune activation on the central nervous system after immunological tolerance in the neonatal period. Methods: Pre-clinical study of the experimental type. 156 male and female C57BL / 6 mice were used. The animals were mated and the offspring used for this study. The collected data were inserted into a database, developed electronically, using the IBM SPSS Statistics 24.0 software. The statistical analysis of the parametric data was performed using the ANOVA test with post-hoc Tukey, and the statistical analysis of the non-parametric data was performed using the Kruskal-Wallis tests. Results: It was observed that the animals that received LPS in the neonatal phase did not present memory deficits and low levels of IL-1b. In the group that received LPS in the neonatal and adult phase, there was an increase in the MDA equivalents and protein carbonylation in an equivalent way to the group of untreated animals. Conclusion: It was found that animals that were subjected to immunological tolerance did not show changes in aversive memory and habituation as well as in IL-1β levels and oxidative damage in brain tissue after receiving an effective dose of LPS.