Avaliação da atividade antioxidante de compostos orgânicos de selênio sob parâmetros de danos oxidativo e mitocondrial em cérebro de ratos submetidos à sepse

• Ano de defesa: 2013
• Autor(a) principal: Silvestre, Fernanda
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Palavras-chave em Português: Sepse; Selênio; Dano oxidativo; Cérebro; Antioxidante
• Link de acesso: https://repositorio.animaeducacao.com.br/handle/ANIMA/3082

The neurological manifestations in patients with acute sepsis as well as cognitive impairment in survivors of sepsis are associated with decreased quality of life and increased mortality and certain aspects of these changes result from pathophysiological mechanisms as oxidative stress. In this context, studies show that antioxidants may play a role in attenuating mechanisms associated with brain dysfunction such as oxidative stress and mitochondrial dysfunction energy. Thus, the literature provides us with important information about the antioxidant role of selenium compounds in the central nervous system, we intend to evaluate the effects of ebselen and diphenyl diselenide in early and late brain dysfunction in rats submitted to an animal model of sepsis. Male Wistar rats were subjected to sepsis by cecal ligation and puncture (CLP), which after treated with oral doses of EB (50 mg / kg), DD (50 mg / kg) or vehicle (oil). Twelve and 24 h after CLP, the rats were sacrificed and samples of brain structures (hippocampus, striatum, cerebellum, and prefrontal cortex) were obtained and analyzed under the parameters of oxidative damage assessment techniques in lipid damage by measuring the formation of thiobarbituric acid reactive substances, protein damage by the formation of carbonyl groups and evaluation of mitochondrial respiratory chain complexes besides the activity of the enzyme creatine kinase. Our results observed in the reduction of oxidative damage to lipids and proteins in the different cerebral structures studied times with the administration of DD, however EB seems to exert the same effect. Such changes are reflected in the assessment of mitochondrial respiratory chain complexes by reversing the decreased activity of the complex caused by the model of CLP. However, for the activity of creatine kinase in 24 hours only positive results found with increased activity through the administration of DD. In this sense, our results suggest that DD may be an important therapeutic target for brain dysfunction associated with sepsis by reversing oxidative damage, dysfunction and energy creatine kinase activity being more effective than EB.