Cultivo in vitro e estaquia de Ginkgo biloba L.
Gorde:
| Egile nagusia: | |
|---|---|
| Argitaratze data: | 2005 |
| Formatua: | Master thesis |
| Hizkuntza: | por |
| Baliabidea: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/9396 |
Gaia: | Ginkgo biloba is an ornamental specie originated from China and has called scientific attention in the west due to the production of chemical compounds as ginkgolides and bilobalide, useful in the treatment of various diseases. The dioecious, the dormancy of the seeds out of their original habitat and the low number of samples available in Brazil, are the limitative factors that justify the development of techniques like the tissue culture and cutting for its multiplication. Aiming to study the behavior of Ginkgo biloba during the in vitro culture and cutting it were carried out five experiments being evaluated the morphogenesis responses and the development of shoot-tips and nodal segments cultured in different culture media; as well the potential rooting of semi hardwood cuttings using different concentrations of growth regulator IBA (indolbutyric acid).It was not possible to establish the in vitro culture from nodal segments due to high rate of fungi contamination. The shoot-tips cultivated on media MS (MURASHIGE and SKOOG,1962)and WPM (LLOYD and McCOWN, 1980) without growth regulator presented the development of shoots from pre-existing shoot-tips, and the MS medium induced higher frequency of shooting and larger number of leaves/explant. Culture media supplemented with the benzilanimopurine (BAP 0, 0.5 1.0 and 2.0 mg L-1 ), kinetin (KIN 0 and 1.0 mg L-1), naftalenoacetic acid (NAA 0 and 0.1 mg L-1), indolacetic acid (IAA 0 and 1.0 mg L-1) and giberelic acid (GA3 0 and 0.5 mg L-1) growth regulators, combined or not with the nutritional addicts, promoted the induction of organogenic callus in 100 % of the shoot-tips cultured. The frequency of organogenic calli, as well the survival of them, were influenced by the composition of the medium, and the combination of KIN+AIA +L-proline+casein hydrolyzed was positive to the induction of this type of callus. In the experiment made with cuttings, the rooting obtained in the 3 doses of IBA tested was 2,5 % |
| _version_ | 1871607650444115968 |
|---|---|
| author | Sexto, Paloma Alves da Silva |
| author_browse | Sexto, Paloma Alves da Silva |
| author_facet | Sexto, Paloma Alves da Silva |
| author_role | author |
| collection | Repositório Institucional da UPF |
| dc.contributor.author.fl_str_mv | Sexto, Paloma Alves da Silva |
| dc.contributor.none.fl_str_mv | Grando, Magali Ferrari Nienow, Alexandre Augusto http://lattes.cnpq.br/7254787776404840 http://lattes.cnpq.br/1711048555843228 |
| dc.date.none.fl_str_mv | 2005-12-22 2025-06-20T16:42:48Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | https://repositorio.upf.br/handle/123456789/9396 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV BR UPF Programa de Pós-Graduação em Agronomia |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UPF instname:Universidade de Passo Fundo (UPF) instacron:UPF |
| dc.subject.por.fl_str_mv | Ginkgo - Cultivo Ginkgo - Propagação por estaquia Ginkgo - Culture Ginkgo - Cuttings CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| dc.title.none.fl_str_mv | Cultivo in vitro e estaquia de Ginkgo biloba L. In vitro culture and cuttings of Ginkgo biloba L. |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Ginkgo biloba is an ornamental specie originated from China and has called scientific attention in the west due to the production of chemical compounds as ginkgolides and bilobalide, useful in the treatment of various diseases. The dioecious, the dormancy of the seeds out of their original habitat and the low number of samples available in Brazil, are the limitative factors that justify the development of techniques like the tissue culture and cutting for its multiplication. Aiming to study the behavior of Ginkgo biloba during the in vitro culture and cutting it were carried out five experiments being evaluated the morphogenesis responses and the development of shoot-tips and nodal segments cultured in different culture media; as well the potential rooting of semi hardwood cuttings using different concentrations of growth regulator IBA (indolbutyric acid).It was not possible to establish the in vitro culture from nodal segments due to high rate of fungi contamination. The shoot-tips cultivated on media MS (MURASHIGE and SKOOG,1962)and WPM (LLOYD and McCOWN, 1980) without growth regulator presented the development of shoots from pre-existing shoot-tips, and the MS medium induced higher frequency of shooting and larger number of leaves/explant. Culture media supplemented with the benzilanimopurine (BAP 0, 0.5 1.0 and 2.0 mg L-1 ), kinetin (KIN 0 and 1.0 mg L-1), naftalenoacetic acid (NAA 0 and 0.1 mg L-1), indolacetic acid (IAA 0 and 1.0 mg L-1) and giberelic acid (GA3 0 and 0.5 mg L-1) growth regulators, combined or not with the nutritional addicts, promoted the induction of organogenic callus in 100 % of the shoot-tips cultured. The frequency of organogenic calli, as well the survival of them, were influenced by the composition of the medium, and the combination of KIN+AIA +L-proline+casein hydrolyzed was positive to the induction of this type of callus. In the experiment made with cuttings, the rooting obtained in the 3 doses of IBA tested was 2,5 % |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | UPF_fd9b8563e1b46c83af76d6fe4b74df10 |
| instacron_str | UPF |
| institution | UPF |
| instname_str | Universidade de Passo Fundo (UPF) |
| language | por |
| network_acronym_str | UPF |
| network_name_str | Repositório Institucional da UPF |
| oai_identifier_str | oai:repositorio.upf.br:123456789/9396 |
| publishDate | 2005 |
| publishDateSort | 2005 |
| publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV BR UPF Programa de Pós-Graduação em Agronomia |
| reponame_str | Repositório Institucional da UPF |
| repository.mail.fl_str_mv | jucelei@upf.br||biblio@upf.br |
| repository.name.fl_str_mv | Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| repository_id_str | 1610 |
| spelling | Cultivo in vitro e estaquia de Ginkgo biloba L.In vitro culture and cuttings of Ginkgo biloba L.Ginkgo - CultivoGinkgo - Propagação por estaquiaGinkgo - CultureGinkgo - CuttingsCNPQ::CIENCIAS AGRARIAS::AGRONOMIAGinkgo biloba is an ornamental specie originated from China and has called scientific attention in the west due to the production of chemical compounds as ginkgolides and bilobalide, useful in the treatment of various diseases. The dioecious, the dormancy of the seeds out of their original habitat and the low number of samples available in Brazil, are the limitative factors that justify the development of techniques like the tissue culture and cutting for its multiplication. Aiming to study the behavior of Ginkgo biloba during the in vitro culture and cutting it were carried out five experiments being evaluated the morphogenesis responses and the development of shoot-tips and nodal segments cultured in different culture media; as well the potential rooting of semi hardwood cuttings using different concentrations of growth regulator IBA (indolbutyric acid).It was not possible to establish the in vitro culture from nodal segments due to high rate of fungi contamination. The shoot-tips cultivated on media MS (MURASHIGE and SKOOG,1962)and WPM (LLOYD and McCOWN, 1980) without growth regulator presented the development of shoots from pre-existing shoot-tips, and the MS medium induced higher frequency of shooting and larger number of leaves/explant. Culture media supplemented with the benzilanimopurine (BAP 0, 0.5 1.0 and 2.0 mg L-1 ), kinetin (KIN 0 and 1.0 mg L-1), naftalenoacetic acid (NAA 0 and 0.1 mg L-1), indolacetic acid (IAA 0 and 1.0 mg L-1) and giberelic acid (GA3 0 and 0.5 mg L-1) growth regulators, combined or not with the nutritional addicts, promoted the induction of organogenic callus in 100 % of the shoot-tips cultured. The frequency of organogenic calli, as well the survival of them, were influenced by the composition of the medium, and the combination of KIN+AIA +L-proline+casein hydrolyzed was positive to the induction of this type of callus. In the experiment made with cuttings, the rooting obtained in the 3 doses of IBA tested was 2,5 %Ginkgo biloba (Ginkgo biloba L.) é uma espécie ornamental originária da China e tem despertado o interesse científico no Ocidente, em função da sua capacidade de sintetizar compostos químicos, como ginkgolides e bilobalide, úteis no tratamento de diversas doenças. A dioicia, a dormência das sementes fora do seu habitat original e o baixo número de exemplares disponíveis no Brasil, são alguns dos fatores limitantes que justificam o desenvolvimento de técnicas como a cultura de tecidos e estaquia para sua multiplicação. Visando estudar o comportamento da Ginkgo biloba durante o cultivo in vitro e estaquia, foram realizados cinco experimentos onde foram avaliadas as respostas morfogenéticas e o desenvolvimento dos ápices caulinares e segmentos nodais cultivados em diferentes meios de cultura, bem como o potencial de enraizamento de estacas semilenhosas utilizando diferentes concentrações do regulador de crescimento AIB (ácido indolbutírico). Não foi possível estabelecer o cultivo in vitro a partir de segmentos nodais devido a elevadas contaminações fúngicas. Os ápices caulinares cultivados em meio MS (MURASHIGE e SKOOG, 1962) e WPM (LLOYD e McCOWN, 1980) sem reguladores de crescimento apresentaram o desenvolvimento de brotações a partir os meristemas pré-existentes, sendo que o meio MS induziu maior freqüência de brotações e número de folhas/explante. Meios suplementados com reguladores de crescimento benzilaminopurina (BAP 0, 0,5 1,0 e 2,0 mg L-1), cinetina (CIN 0, e 1,0 mg L-1 ) combinados com ácido naftalenoacético (ANA 0 e 0,1 mg L-1) e ácido indolacético (AIA 0 e 1,0 mg L-1) e ácido giberélico (GA3 0 e 0,5 mg L-1 ), suplementados ou não com aditivos nutricionais, induziram a produção de calos em 100 % dos ápices caulinares. A freqüência de calos organogênicos, bem como a sobrevivência dos mesmos foi influenciada pela composição do meio de cultura, sendo a combinação de KIN+AIA+L-prolina+caseína hidrolizada positiva para a indução deste tipo de calo. No experimento realizado com estaquia, o enraizamento obtido nas três doses de AIB testadas, foi de 2,5 %Universidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBRUPFPrograma de Pós-Graduação em AgronomiaGrando, Magali FerrariNienow, Alexandre Augustohttp://lattes.cnpq.br/7254787776404840http://lattes.cnpq.br/1711048555843228Sexto, Paloma Alves da Silva2025-06-20T16:42:48Z2005-12-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfhttps://repositorio.upf.br/handle/123456789/9396porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-12-30T20:37:51Zoai:repositorio.upf.br:123456789/9396Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-12-30T20:37:51Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| spellingShingle | Cultivo in vitro e estaquia de Ginkgo biloba L. Sexto, Paloma Alves da Silva Ginkgo - Cultivo Ginkgo - Propagação por estaquia Ginkgo - Culture Ginkgo - Cuttings CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| status_str | publishedVersion |
| title | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| title_full | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| title_fullStr | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| title_full_unstemmed | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| title_short | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| title_sort | Cultivo in vitro e estaquia de Ginkgo biloba L. |
| topic | Ginkgo - Cultivo Ginkgo - Propagação por estaquia Ginkgo - Culture Ginkgo - Cuttings CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| url | https://repositorio.upf.br/handle/123456789/9396 |