Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares
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| 1. Verfasser: | |
|---|---|
| Publikationsdatum: | 2018 |
| Format: | Master thesis |
| Sprache: | por |
| Quelle: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/9230 |
Zusammenfassung: | The objective of this work was to characterize the high molecular weight glutenin subunits (HMW-GS) and to analyze their relation with parameters of wheat technological quality in a collection of wheat genotypes with importance in the introgression of characteristics of interest by breeding programs in Brazil. A total of 274 accessions of wheats were evaluated by SDSPAGE, and for 219 accessions with homogeneity for HMW-GS 53 profiles and 21 alleles were found. The most frequent combination was 2 */7+9/5+10 (11.9%). Based on the protein profile of HMW-GS and the identification of 7oe allele overexpression and 1BL.1RS translocation with DNA molecular markers, the Glu-1 score was calculated and the correlations with wheat technological quality parameters were analyzed. The main effects of the Glu-1 and 1BL.1RS loci were estimated using the maximum likelihood-restricted algorithm (REML). The Glu-1 score showed significant correlations with the QTT: sodium dodecylsulfate microssedimentation (MSSDS);alveography with gluten strength (W), tenacity (P), extensibility (L) and elasticity index (Ie) and pharynography, with the parameter stability (ST) with values of correlation coefficient (r) varying from 0.27 to 0.51. Alleles 1, 7oe+8 and 5+10, located at the Glu-A1, Glu-B1 and GluD1 loci, respectively, had higher values for W, Ie and ST. The results indicated that an adjustment to the Glu-A1 locus alleles score for Brazil conditions of cultivation is required as well as the use of molecular markers for the identification of the 7oe+8 combination. SNP (single nucleotide polymorphism) molecular markers were used to analyze 467 accessions of wheat by the KASP method for alleles of the Glu-A1 and Glu-D1 loci and for translocation 1BL.1RS. The SDS-PAGE analysis of 270 of the accessions allowed the comparison of the results with the DNA analyzes. For the Glu-A1 locus marker there was 91.8% agreement with the protein profile of HMW-GS and for locus Glu-D1, 98.8%. For the Glu-B1 locus marker of superexpression 7oe and 22 (10%) were identified with this allele. The presence of 1BL.1RS translocation was identified in 83 (18%) of the 467 accessions of wheat. The results indicated the efficiency in the use of molecular markers for the Glu-1 loci. |
| _version_ | 1871607649336819712 |
|---|---|
| author | Vancini, Camila |
| author_browse | Vancini, Camila |
| author_facet | Vancini, Camila |
| author_role | author |
| collection | Repositório Institucional da UPF |
| dc.contributor.author.fl_str_mv | Vancini, Camila |
| dc.contributor.none.fl_str_mv | Grando, Magali Ferrari http://lattes.cnpq.br/7254787776404840 http://lattes.cnpq.br/1681035279707004 Torres, Gisele Abigail Montan |
| dc.date.none.fl_str_mv | 2018-04-30 2025-06-20T16:41:41Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | VANCINI, Camila. Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares. 2018. 112 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2018. https://repositorio.upf.br/handle/123456789/9230 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Agronomia |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UPF instname:Universidade de Passo Fundo (UPF) instacron:UPF |
| dc.subject.por.fl_str_mv | Trigo Marcadores genéticos Panificação CIENCIAS AGRARIAS::AGRONOMIA |
| dc.title.none.fl_str_mv | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares Characterization of high molecular weight glutenins alleles, influence on wheat end use quality in Brazil and analysis of molecular markers |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | The objective of this work was to characterize the high molecular weight glutenin subunits (HMW-GS) and to analyze their relation with parameters of wheat technological quality in a collection of wheat genotypes with importance in the introgression of characteristics of interest by breeding programs in Brazil. A total of 274 accessions of wheats were evaluated by SDSPAGE, and for 219 accessions with homogeneity for HMW-GS 53 profiles and 21 alleles were found. The most frequent combination was 2 */7+9/5+10 (11.9%). Based on the protein profile of HMW-GS and the identification of 7oe allele overexpression and 1BL.1RS translocation with DNA molecular markers, the Glu-1 score was calculated and the correlations with wheat technological quality parameters were analyzed. The main effects of the Glu-1 and 1BL.1RS loci were estimated using the maximum likelihood-restricted algorithm (REML). The Glu-1 score showed significant correlations with the QTT: sodium dodecylsulfate microssedimentation (MSSDS);alveography with gluten strength (W), tenacity (P), extensibility (L) and elasticity index (Ie) and pharynography, with the parameter stability (ST) with values of correlation coefficient (r) varying from 0.27 to 0.51. Alleles 1, 7oe+8 and 5+10, located at the Glu-A1, Glu-B1 and GluD1 loci, respectively, had higher values for W, Ie and ST. The results indicated that an adjustment to the Glu-A1 locus alleles score for Brazil conditions of cultivation is required as well as the use of molecular markers for the identification of the 7oe+8 combination. SNP (single nucleotide polymorphism) molecular markers were used to analyze 467 accessions of wheat by the KASP method for alleles of the Glu-A1 and Glu-D1 loci and for translocation 1BL.1RS. The SDS-PAGE analysis of 270 of the accessions allowed the comparison of the results with the DNA analyzes. For the Glu-A1 locus marker there was 91.8% agreement with the protein profile of HMW-GS and for locus Glu-D1, 98.8%. For the Glu-B1 locus marker of superexpression 7oe and 22 (10%) were identified with this allele. The presence of 1BL.1RS translocation was identified in 83 (18%) of the 467 accessions of wheat. The results indicated the efficiency in the use of molecular markers for the Glu-1 loci. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | UPF_f66aab7ff2d8d586994516cfc87f17cc |
| identifier_str_mv | VANCINI, Camila. Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares. 2018. 112 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2018. |
| instacron_str | UPF |
| institution | UPF |
| instname_str | Universidade de Passo Fundo (UPF) |
| language | por |
| network_acronym_str | UPF |
| network_name_str | Repositório Institucional da UPF |
| oai_identifier_str | oai:repositorio.upf.br:123456789/9230 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Agronomia |
| reponame_str | Repositório Institucional da UPF |
| repository.mail.fl_str_mv | jucelei@upf.br||biblio@upf.br |
| repository.name.fl_str_mv | Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| repository_id_str | 1610 |
| spelling | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores molecularesCharacterization of high molecular weight glutenins alleles, influence on wheat end use quality in Brazil and analysis of molecular markersTrigoMarcadores genéticosPanificaçãoCIENCIAS AGRARIAS::AGRONOMIAThe objective of this work was to characterize the high molecular weight glutenin subunits (HMW-GS) and to analyze their relation with parameters of wheat technological quality in a collection of wheat genotypes with importance in the introgression of characteristics of interest by breeding programs in Brazil. A total of 274 accessions of wheats were evaluated by SDSPAGE, and for 219 accessions with homogeneity for HMW-GS 53 profiles and 21 alleles were found. The most frequent combination was 2 */7+9/5+10 (11.9%). Based on the protein profile of HMW-GS and the identification of 7oe allele overexpression and 1BL.1RS translocation with DNA molecular markers, the Glu-1 score was calculated and the correlations with wheat technological quality parameters were analyzed. The main effects of the Glu-1 and 1BL.1RS loci were estimated using the maximum likelihood-restricted algorithm (REML). The Glu-1 score showed significant correlations with the QTT: sodium dodecylsulfate microssedimentation (MSSDS);alveography with gluten strength (W), tenacity (P), extensibility (L) and elasticity index (Ie) and pharynography, with the parameter stability (ST) with values of correlation coefficient (r) varying from 0.27 to 0.51. Alleles 1, 7oe+8 and 5+10, located at the Glu-A1, Glu-B1 and GluD1 loci, respectively, had higher values for W, Ie and ST. The results indicated that an adjustment to the Glu-A1 locus alleles score for Brazil conditions of cultivation is required as well as the use of molecular markers for the identification of the 7oe+8 combination. SNP (single nucleotide polymorphism) molecular markers were used to analyze 467 accessions of wheat by the KASP method for alleles of the Glu-A1 and Glu-D1 loci and for translocation 1BL.1RS. The SDS-PAGE analysis of 270 of the accessions allowed the comparison of the results with the DNA analyzes. For the Glu-A1 locus marker there was 91.8% agreement with the protein profile of HMW-GS and for locus Glu-D1, 98.8%. For the Glu-B1 locus marker of superexpression 7oe and 22 (10%) were identified with this allele. The presence of 1BL.1RS translocation was identified in 83 (18%) of the 467 accessions of wheat. The results indicated the efficiency in the use of molecular markers for the Glu-1 loci.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESO objetivo desse trabalho foi caracterizar as gluteninas de alto peso molecular (HMW-GS) e analisar sua relação com parâmetros de qualidade tecnológica de trigo (QTT) em uma coleção de genótipos de trigo com importância na introgressão de características de interesse pelos programas de melhoramento no Brasil. Foram avaliados 274 acessos de trigo por SDS-PAGE, sendo que para 219 acessos com homogeneidade para gluteninas de alto peso molecular foram encontrados 53 perfis e 21 alelos. A combinação mais frequente foi 2*/7+9/5+10 (11,9%). Com base no perfil proteico de HMW-GS e na identificação da super expressão do alelo 7oe e da translocação 1BL.1RS, através do uso de marcadores moleculares de DNA, o escore Glu-1 foi calculado e as correlações com parâmetros de qualidade tecnológica de trigo (QTT) foram analisadas. Os principais efeitos dos locos Glu-1 e 1BL.1RS foram estimados usando o algoritmo da máxima verossimilhança restrita (REML). O escore Glu-1 mostrou correlações significativas com as análises de QTT: microssedimentação com dodecil-sulfato de sódio (MS-SDS); alveografia, com os parâmetros: força de glúten (W), tenacidade (P), extensibilidade (L) e índice de elasticidade (Ie) e farinografia, com o parâmetro estabilidade (EST) com valores de coeficiente de correlação (r) variando de 0,27 a 0,51. Os alelos 1, 7oe+8 e 5+10, localizados nos locos Glu- A1, Glu-B1 e Glu-D1, respectivamente, tiveram maiores valores para W, Ie e EST. Os resultados indicaram que é necessário um ajuste no escore para os alelos do loco Glu-A1 para as condições do Brasil bem como o uso de marcadores moleculares para a identificação da combinação 7oe+8. Marcadores moleculares do tipo SNP (single nucleotide polymorphism) foram usados para analisar 467 acessos de trigo pelo método KASP, para alelos dos locos Glu-A1 e Glu-D1 e para translocação 1BL.1RS. A análise de HMW-GS em SDS-PAGE de 270 dos acessos possibilitou a comparação dos resultados com as análises de DNA. Para o marcador do loco Glu-A1 houve 91,8% de concordância com o perfil proteico de HMW-GS e para o loco Glu-D1, 98,8%. Para o marcador do loco Glu-B1 da super expressão 7oe, foram identificados 22 (10%) acessos com este alelo. A presença da translocação 1BL.1RS foi identificada em 83 (18%) dos 467 acessos de trigo avaliados. Os resultados indicaram a eficiência no uso de marcadores moleculares para os locos Glu-1.Universidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBrasilUPFPrograma de Pós-Graduação em AgronomiaGrando, Magali Ferrarihttp://lattes.cnpq.br/7254787776404840http://lattes.cnpq.br/1681035279707004Torres, Gisele Abigail MontanVancini, Camila2025-06-20T16:41:41Z2018-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfVANCINI, Camila. Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares. 2018. 112 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2018.https://repositorio.upf.br/handle/123456789/9230porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-12-30T20:09:46Zoai:repositorio.upf.br:123456789/9230Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-12-30T20:09:46Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| spellingShingle | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares Vancini, Camila Trigo Marcadores genéticos Panificação CIENCIAS AGRARIAS::AGRONOMIA |
| status_str | publishedVersion |
| title | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| title_full | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| title_fullStr | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| title_full_unstemmed | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| title_short | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| title_sort | Caracterização de alelos de gluteninas de alto peso molecular, influência sobre a qualidade de uso final de trigo no Brasil e análise de marcadores moleculares |
| topic | Trigo Marcadores genéticos Panificação CIENCIAS AGRARIAS::AGRONOMIA |
| url | https://repositorio.upf.br/handle/123456789/9230 |