Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha
Gorde:
| Egile nagusia: | |
|---|---|
| Argitaratze data: | 2015 |
| Formatua: | Doctoral thesis |
| Hizkuntza: | por |
| Baliabidea: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/9340 |
Gaia: | Wheat leaf rust is caused by the fungus Puccinia triticina and is one of the major diseases which annually affect the wheat crop in southern Brazil. The use of cultivars with durable genetic resistance has been essential to the progress of the culture. Thus, this study investigated which genes, mechanisms and metabolic pathways are involved in the durable adult plant resistance (APR) of wheat cv. Toropi. At the Brazilian Agricultural Research Corporation (Embrapa Trigo), wheat plants of the cv. Toropi were cultivated in growth chambers with controlled environment; inoculation of flag leaves with B55 race (MDT-MR) of P. triticina (MDT-MR) was performed; flag leaves were collected in different time-points postinoculation (0, 1, 3, 6, 12, 24, 48 and 72 hours after inoculation) and the RNA was extracted and purified. Analyses of gene expression by RT-qPCR were performed at the National Institute of Agricultural Botany (NIAB) in Cambridge/England, during the completion of doctorate sandwich. The sequencing of the post-inoculation timepoints was performed by The Genome Analysis Centre (TGAC), Norwich/England. Analyses of bioinformatics were conducted in two stages, in NIAB during the completion of sandwich doctorate, and after at the multi-user laboratory of Embrapa Informática Agropecuária CNPTIA and Embrapa Trigo. The expression analysis using gene primers related to the plant defense in wheat by RT-qPCR showed that classical defense mechanisms as lignification, oxidative stress, energy generation, lipids and water flow and cell cycle were altered during the process of infection and may be pathogen modulated in order to benefit its development. In sequencing and bioinformatics of the time-points 0, 6, 12 and 24 hai were obtained 463.371 contigs of which 19.633 were differentially expressed over time. The main metabolic pathways altered during the process of infection by leaf rust were generation of energy and metabolic precursors; catabolism; response to biotic and abiotic stresses; and transportation. Twelve of Toropi contigs were expressed only in the presence of the pathogen and not have homology in cv. Chinese Spring, may represent essential genes to the defense process. The understanding of this resistance enables their use in genetic improvement of wheat in order to obtain cultivars with durable genetic resistance |
| _version_ | 1871607637998567424 |
|---|---|
| author | Casassola, Alice |
| author_browse | Casassola, Alice |
| author_facet | Casassola, Alice |
| author_role | author |
| collection | Repositório Institucional da UPF |
| dc.contributor.author.fl_str_mv | Casassola, Alice |
| dc.contributor.none.fl_str_mv | Brammer, Sandra Patussi http://lattes.cnpq.br/2148828037195161 Chaves, Márcia Soares |
| dc.date.none.fl_str_mv | 2015-04-10 2025-06-20T16:42:27Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | CASASSOLA, Alice. Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha. 2015. 142 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2015. https://repositorio.upf.br/handle/123456789/9340 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV BR UPF Programa de Pós-Graduação em Agronomia |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UPF instname:Universidade de Passo Fundo (UPF) instacron:UPF |
| dc.subject.por.fl_str_mv | Trigo - Melhoramento genético Fungos fitopatogênicos - Plantas hospedeiras Trigo Wheat - genetic improvement Fungi pathogenic - Host plants Wheat CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| dc.title.none.fl_str_mv | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha Gene expression in wheat in response to puccinia triticina infection, causal agent of leaf rust |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/doctoralThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Wheat leaf rust is caused by the fungus Puccinia triticina and is one of the major diseases which annually affect the wheat crop in southern Brazil. The use of cultivars with durable genetic resistance has been essential to the progress of the culture. Thus, this study investigated which genes, mechanisms and metabolic pathways are involved in the durable adult plant resistance (APR) of wheat cv. Toropi. At the Brazilian Agricultural Research Corporation (Embrapa Trigo), wheat plants of the cv. Toropi were cultivated in growth chambers with controlled environment; inoculation of flag leaves with B55 race (MDT-MR) of P. triticina (MDT-MR) was performed; flag leaves were collected in different time-points postinoculation (0, 1, 3, 6, 12, 24, 48 and 72 hours after inoculation) and the RNA was extracted and purified. Analyses of gene expression by RT-qPCR were performed at the National Institute of Agricultural Botany (NIAB) in Cambridge/England, during the completion of doctorate sandwich. The sequencing of the post-inoculation timepoints was performed by The Genome Analysis Centre (TGAC), Norwich/England. Analyses of bioinformatics were conducted in two stages, in NIAB during the completion of sandwich doctorate, and after at the multi-user laboratory of Embrapa Informática Agropecuária CNPTIA and Embrapa Trigo. The expression analysis using gene primers related to the plant defense in wheat by RT-qPCR showed that classical defense mechanisms as lignification, oxidative stress, energy generation, lipids and water flow and cell cycle were altered during the process of infection and may be pathogen modulated in order to benefit its development. In sequencing and bioinformatics of the time-points 0, 6, 12 and 24 hai were obtained 463.371 contigs of which 19.633 were differentially expressed over time. The main metabolic pathways altered during the process of infection by leaf rust were generation of energy and metabolic precursors; catabolism; response to biotic and abiotic stresses; and transportation. Twelve of Toropi contigs were expressed only in the presence of the pathogen and not have homology in cv. Chinese Spring, may represent essential genes to the defense process. The understanding of this resistance enables their use in genetic improvement of wheat in order to obtain cultivars with durable genetic resistance |
| eu_rights_str_mv | openAccess |
| format | doctoralThesis |
| id | UPF_993b7308c8f33b36ff8b80fbc6b4ef3e |
| identifier_str_mv | CASASSOLA, Alice. Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha. 2015. 142 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2015. |
| instacron_str | UPF |
| institution | UPF |
| instname_str | Universidade de Passo Fundo (UPF) |
| language | por |
| network_acronym_str | UPF |
| network_name_str | Repositório Institucional da UPF |
| oai_identifier_str | oai:repositorio.upf.br:123456789/9340 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV BR UPF Programa de Pós-Graduação em Agronomia |
| reponame_str | Repositório Institucional da UPF |
| repository.mail.fl_str_mv | jucelei@upf.br||biblio@upf.br |
| repository.name.fl_str_mv | Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| repository_id_str | 1610 |
| spelling | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folhaGene expression in wheat in response to puccinia triticina infection, causal agent of leaf rustTrigo - Melhoramento genéticoFungos fitopatogênicos - Plantas hospedeirasTrigoWheat - genetic improvementFungi pathogenic - Host plantsWheatCNPQ::CIENCIAS AGRARIAS::AGRONOMIAWheat leaf rust is caused by the fungus Puccinia triticina and is one of the major diseases which annually affect the wheat crop in southern Brazil. The use of cultivars with durable genetic resistance has been essential to the progress of the culture. Thus, this study investigated which genes, mechanisms and metabolic pathways are involved in the durable adult plant resistance (APR) of wheat cv. Toropi. At the Brazilian Agricultural Research Corporation (Embrapa Trigo), wheat plants of the cv. Toropi were cultivated in growth chambers with controlled environment; inoculation of flag leaves with B55 race (MDT-MR) of P. triticina (MDT-MR) was performed; flag leaves were collected in different time-points postinoculation (0, 1, 3, 6, 12, 24, 48 and 72 hours after inoculation) and the RNA was extracted and purified. Analyses of gene expression by RT-qPCR were performed at the National Institute of Agricultural Botany (NIAB) in Cambridge/England, during the completion of doctorate sandwich. The sequencing of the post-inoculation timepoints was performed by The Genome Analysis Centre (TGAC), Norwich/England. Analyses of bioinformatics were conducted in two stages, in NIAB during the completion of sandwich doctorate, and after at the multi-user laboratory of Embrapa Informática Agropecuária CNPTIA and Embrapa Trigo. The expression analysis using gene primers related to the plant defense in wheat by RT-qPCR showed that classical defense mechanisms as lignification, oxidative stress, energy generation, lipids and water flow and cell cycle were altered during the process of infection and may be pathogen modulated in order to benefit its development. In sequencing and bioinformatics of the time-points 0, 6, 12 and 24 hai were obtained 463.371 contigs of which 19.633 were differentially expressed over time. The main metabolic pathways altered during the process of infection by leaf rust were generation of energy and metabolic precursors; catabolism; response to biotic and abiotic stresses; and transportation. Twelve of Toropi contigs were expressed only in the presence of the pathogen and not have homology in cv. Chinese Spring, may represent essential genes to the defense process. The understanding of this resistance enables their use in genetic improvement of wheat in order to obtain cultivars with durable genetic resistanceA ferrugem-da-folha do trigo é causada pelo fungo Puccinia triticina e é uma das principais doenças que acometem anualmente a cultura do trigo na região sul do Brasil. O uso de cultivares com resistência genética durável tem sido essencial para o avanço da cultura. Dessa forma, este trabalho objetivou estudar quais os genes, mecanismos e rotas metabólicas estão envolvidos na resistência de planta adulta (RPA) durável da cultivar Toropi. Na Empresa Brasileira de Pesquisa Agropecuária (Embrapa Trigo), plantas de trigo da cv. Toropi foram cultivadas em câmaras de crescimento com ambiente controlado; foi realizada a inoculação das folhas bandeira com a raça B55 de P. triticina (MDT-MR); as folhas bandeiras nos diferentes horários pós-inoculação (0, 1, 3, 6, 12, 24, 48 e 72 horas após a inoculação) foram coletadas e o RNA foi extraído purificado. As análises de expressão gênica mediante RT-qPCR foram realizadas no National Institute of Agricultural Botany (NIAB), em Cambridge/Inglaterra, durante a realização de doutorado sanduíche. O CNPTIA e na Embrapa Trigo. As análises de expressão utilizando-se iniciadores de genes relacionados com a defesa em trigo mediante RTqPCR mostraram que mecanismos clássicos de defesa como lignificação, estresse oxidativo, geração de energia, fluxo de água e lipídeos e ciclo celular, foram alterados durante o processo de infecção, bem como pode haver uma modulação do metabolismo da planta pelo patógeno visando beneficiar seu desenvolvimento. No sequenciamento e bioinformática dos tempos 0, 6, 12 e 24 hai foram obtidos 463.371 contigs dos quais 19.633 foram diferencialmente expressos ao longo do tempo. As principais rotas metabólicas alteradas durante o processo de infecção por ferrugem-da-folha em Toropi foram geração de precursores metabólicos e energia; catabolismo; resposta a estímulos bióticos e abióticos e ao estresse; e transporte. Doze contigs de Toropi foram expressos somente na presença do patógeno e não possuem homologia na cv. Chinese Spring, podendo representar genes imprescindíveis no processo de defesa. O entendimento dessa resistência viabiliza seu uso no melhoramento genético do trigo visando à obtenção de cultivares com resistência genética durávelUniversidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBRUPFPrograma de Pós-Graduação em AgronomiaBrammer, Sandra Patussihttp://lattes.cnpq.br/2148828037195161Chaves, Márcia SoaresCasassola, Alice2025-06-20T16:42:27Z2015-04-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfCASASSOLA, Alice. Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha. 2015. 142 f. Tese (Doutorado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, 2015.https://repositorio.upf.br/handle/123456789/9340porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-12-30T20:27:25Zoai:repositorio.upf.br:123456789/9340Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-12-30T20:27:25Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| spellingShingle | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha Casassola, Alice Trigo - Melhoramento genético Fungos fitopatogênicos - Plantas hospedeiras Trigo Wheat - genetic improvement Fungi pathogenic - Host plants Wheat CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| status_str | publishedVersion |
| title | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| title_full | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| title_fullStr | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| title_full_unstemmed | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| title_short | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| title_sort | Expressão gênica em trigo em resposta a infecção por puccinia triticina, agente casual da ferrugem da folha |
| topic | Trigo - Melhoramento genético Fungos fitopatogênicos - Plantas hospedeiras Trigo Wheat - genetic improvement Fungi pathogenic - Host plants Wheat CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
| url | https://repositorio.upf.br/handle/123456789/9340 |