Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio)
שמור ב:
| מחבר ראשי: | |
|---|---|
| Publication Date: | 2015 |
| פורמט: | Master thesis |
| שפה: | por |
| Source: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/1575 |
סיכום: | Contamination of water resources be they municipal wastewater or surface water, by drugs and their metabolites have been reported since the 70s, endangering the user population of these resources as well as the aquatic habitat. Risperidone, an atypical antipsychotic used to treat patients with schizophrenia, psychosis, Alzheimer's disease and autism found in wastewater in environmental concentration 0,00034μg/L. This drug possesses anxiolytic and antidepressant power but to the development of this experiment are not sure if it could be somehow interfering with the neuroendocrine system of fish stress. The activation of the hypothalamic-pituitary-interrenal axis (HPI) and consequent elevation of cortisol in fish occur when exposed to an attacker, be it predator, reduced oxygen or contamination by drugs. In zebrafish the stress response has similarity to those of terrestrial vertebrates in that it involves consumption and oxygen transfer, energy mobilization, growth, reproduction and effects on immune functions. The zebrafish was used because of its rapid growth and ease of maintenance, as well as its homology with humans, more studies with toxicology, pharmacology and behavior. This study was conducted at the Fish Physiology Laboratory, Faculty of Agronomy and Veterinary Medicine (FAMV), University of Passo Fundo (UPF), first evaluated front cortisol levels to five different concentrations (0,00034, 8, 170, 340 and 680μg/L) and risperidone on four different times (-15, 15, 60 and 240'), with acute drug exposure(15'). As result is found is that risperidone in the presence of water interferes with the HPI axis at a concentration of 170μg/L in time 15 minutes after stress, continuing then to the second part of the experiment where the zebrafish behavior was evaluated in concentration effect. The observed parameters were the distance traveled, average speed,the absolute angle turn, the number of intersections between the lower compartments, secondary and higher and the length of stay in the lower compartments, secondary and higher. In animal behavior test remained along the bottom of comparing the stress with the stress more risperidone , can be considered , risperidone, an anxiolytic . |
| _version_ | 1871607619915874304 |
|---|---|
| author | Idalencio, Renan |
| author_browse | Idalencio, Renan |
| author_facet | Idalencio, Renan |
| author_role | author |
| collection | Repositório Institucional da UPF |
| dc.contributor.author.fl_str_mv | Idalencio, Renan |
| dc.contributor.none.fl_str_mv | Barcellos, Leonardo José Gil http://lattes.cnpq.br/4504482634911546 |
| dc.date.none.fl_str_mv | 2015-07-14 2025-05-07T00:27:02Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | IDALENCIO, Renan. Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio). 2015. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2015. https://repositorio.upf.br/handle/123456789/1575 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Bioexperimentação |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UPF instname:Universidade de Passo Fundo (UPF) instacron:UPF |
| dc.subject.por.fl_str_mv | Recursos hídricos Contaminação microbiana Peixe de água doce Peixe - Efeito do stress CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| dc.title.none.fl_str_mv | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) Risperidone decreases the response to stress in zebrafish (Danio rerio) |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Contamination of water resources be they municipal wastewater or surface water, by drugs and their metabolites have been reported since the 70s, endangering the user population of these resources as well as the aquatic habitat. Risperidone, an atypical antipsychotic used to treat patients with schizophrenia, psychosis, Alzheimer's disease and autism found in wastewater in environmental concentration 0,00034μg/L. This drug possesses anxiolytic and antidepressant power but to the development of this experiment are not sure if it could be somehow interfering with the neuroendocrine system of fish stress. The activation of the hypothalamic-pituitary-interrenal axis (HPI) and consequent elevation of cortisol in fish occur when exposed to an attacker, be it predator, reduced oxygen or contamination by drugs. In zebrafish the stress response has similarity to those of terrestrial vertebrates in that it involves consumption and oxygen transfer, energy mobilization, growth, reproduction and effects on immune functions. The zebrafish was used because of its rapid growth and ease of maintenance, as well as its homology with humans, more studies with toxicology, pharmacology and behavior. This study was conducted at the Fish Physiology Laboratory, Faculty of Agronomy and Veterinary Medicine (FAMV), University of Passo Fundo (UPF), first evaluated front cortisol levels to five different concentrations (0,00034, 8, 170, 340 and 680μg/L) and risperidone on four different times (-15, 15, 60 and 240'), with acute drug exposure(15'). As result is found is that risperidone in the presence of water interferes with the HPI axis at a concentration of 170μg/L in time 15 minutes after stress, continuing then to the second part of the experiment where the zebrafish behavior was evaluated in concentration effect. The observed parameters were the distance traveled, average speed,the absolute angle turn, the number of intersections between the lower compartments, secondary and higher and the length of stay in the lower compartments, secondary and higher. In animal behavior test remained along the bottom of comparing the stress with the stress more risperidone , can be considered , risperidone, an anxiolytic . |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | UPF_3791333c013fa4b7e547fdcda1cf24b6 |
| identifier_str_mv | IDALENCIO, Renan. Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio). 2015. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2015. |
| instacron_str | UPF |
| institution | UPF |
| instname_str | Universidade de Passo Fundo (UPF) |
| language | por |
| network_acronym_str | UPF |
| network_name_str | Repositório Institucional da UPF |
| oai_identifier_str | oai:repositorio.upf.br:123456789/1575 |
| publishDate | 2015 |
| publishDateSort | 2015 |
| publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Bioexperimentação |
| reponame_str | Repositório Institucional da UPF |
| repository.mail.fl_str_mv | jucelei@upf.br||biblio@upf.br |
| repository.name.fl_str_mv | Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| repository_id_str | 1610 |
| spelling | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio)Risperidone decreases the response to stress in zebrafish (Danio rerio)Recursos hídricosContaminação microbianaPeixe de água docePeixe - Efeito do stressCIENCIAS AGRARIAS::MEDICINA VETERINARIAContamination of water resources be they municipal wastewater or surface water, by drugs and their metabolites have been reported since the 70s, endangering the user population of these resources as well as the aquatic habitat. Risperidone, an atypical antipsychotic used to treat patients with schizophrenia, psychosis, Alzheimer's disease and autism found in wastewater in environmental concentration 0,00034μg/L. This drug possesses anxiolytic and antidepressant power but to the development of this experiment are not sure if it could be somehow interfering with the neuroendocrine system of fish stress. The activation of the hypothalamic-pituitary-interrenal axis (HPI) and consequent elevation of cortisol in fish occur when exposed to an attacker, be it predator, reduced oxygen or contamination by drugs. In zebrafish the stress response has similarity to those of terrestrial vertebrates in that it involves consumption and oxygen transfer, energy mobilization, growth, reproduction and effects on immune functions. The zebrafish was used because of its rapid growth and ease of maintenance, as well as its homology with humans, more studies with toxicology, pharmacology and behavior. This study was conducted at the Fish Physiology Laboratory, Faculty of Agronomy and Veterinary Medicine (FAMV), University of Passo Fundo (UPF), first evaluated front cortisol levels to five different concentrations (0,00034, 8, 170, 340 and 680μg/L) and risperidone on four different times (-15, 15, 60 and 240'), with acute drug exposure(15'). As result is found is that risperidone in the presence of water interferes with the HPI axis at a concentration of 170μg/L in time 15 minutes after stress, continuing then to the second part of the experiment where the zebrafish behavior was evaluated in concentration effect. The observed parameters were the distance traveled, average speed,the absolute angle turn, the number of intersections between the lower compartments, secondary and higher and the length of stay in the lower compartments, secondary and higher. In animal behavior test remained along the bottom of comparing the stress with the stress more risperidone , can be considered , risperidone, an anxiolytic .A contaminacao de recursos hidricos sejam eles efluentes municipais ou aguas de superficie, por farmacos e/ou seus metabolitos, tem sido relatada desde a decada de 70, colocando em risco a populacao usuaria desses recursos assim como o habitat aquatico. A risperidona, um antipsicotico atipico, usada para o tratamento de pacientes com esquizofrenia, psicoses, Alzheimer e autismo, foi encontrada nas aguas residuais na concentracao ambiental de 0,00034ƒÊg/L. Este medicamento possui poder ansiolitico e antidepressivo porem ate o desenvolvimento deste experimento nao se sabia ao certo se poderia estar de alguma forma interferindo no sistema neuroendocrino de estresse dos peixes. A ativacao do eixo hipotalamo-hipofise-interrenal (HPI) e consequente elevacao do cortisol nos peixes ocorrem quando em contato a um agressor, seja ele predador, reducao de oxigenio ou contaminacao por farmacos. No zebrafish a resposta ao estresse apresenta similaridade aos dos vertebrados terrestres no que envolve consumo e transferencia de oxigenio, mobilizacao de energia, crescimento, reproducao e efeitos nas funcoes imunes. O zebrafish foi utilizado devido ao seu rapido crescimento e facilidade de manutencao, assim como sua homologia com seres humanos, crescentes estudos com toxicologia, farmacologia e comportamento. O presente trabalho foi desenvolvido no Laboratorio de Fisiologia de Peixes, Faculdade de Agronomia e Medicina Veterinaria (FAMV), Universidade de Passo Fundo (UPF), avaliou primeiramente os niveis de cortisol frente a cinco diferentes concentracoes (0,00034, 85, 170, 340 e 680ƒÊg/L) de risperidona e em quatro diferentes tempos (-15, 15, 60 e 240 f), com exposicao aguda ao farmaco (15 f). Como resultado constatou-se que a presenca da risperidona na agua interfere no eixo HPI na concentracao de 170ƒÊg/L no tempo de 15 minutos apos estresse, dando continuidade entao para a segunda parte do experimento onde o comportamento do zebrafish foi avaliado na concentracao de efeito. Os parametros observados foram a distancia percorrida, a velocidade media, o angulo absoluto de virada, o numero de cruzamentos entre os compartimentos inferior, medio e superior e o tempo de permanencia nos compartimentos inferior, medio e superior. No teste de comportamento os animais permaneceram maior tempo no fundo comparando estresse com estresse mais risperidona, podendo ser considerada, a risperidona, um ansiolitico.Universidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBrasilUPFPrograma de Pós-Graduação em BioexperimentaçãoBarcellos, Leonardo José Gilhttp://lattes.cnpq.br/4504482634911546Idalencio, Renan2025-05-07T00:27:02Z2015-07-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfIDALENCIO, Renan. Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio). 2015. 39 f. Dissertação (Mestrado em Bioexperimentação) - Universidade de Passo Fundo, Passo Fundo, RS, 2015.https://repositorio.upf.br/handle/123456789/1575porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-12-30T20:28:56Zoai:repositorio.upf.br:123456789/1575Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-12-30T20:28:56Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| spellingShingle | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) Idalencio, Renan Recursos hídricos Contaminação microbiana Peixe de água doce Peixe - Efeito do stress CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| status_str | publishedVersion |
| title | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| title_full | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| title_fullStr | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| title_full_unstemmed | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| title_short | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| title_sort | Risperidona diminui a resposta ap estresse em peixe-zebra (Danio rerio) |
| topic | Recursos hídricos Contaminação microbiana Peixe de água doce Peixe - Efeito do stress CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
| url | https://repositorio.upf.br/handle/123456789/1575 |