Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante
Gardado en:
| Autor Principal: | |
|---|---|
| Data de Publicación: | 2019 |
| Formato: | Master thesis |
| Idioma: | por |
| Fonte: | Repositório Institucional da UPF |
| Download full: | https://repositorio.upf.br/handle/123456789/9258 |
Summary: | Water deficit stress is the one of main occurrence and the one that most affects plant development. Fungicide application protects plants, but can lead to oxidative stress because it is a xenobiotic, compound strange to the plant. Under field conditions, plants are exposed to combinations of biotic and abiotic stresses, such as combined deficit water and fungicide application. The challenge of scientific research is characterize these processes and create management strategies to mitigate metabolic and economic effects. Also, to minimize the impacts of these stresses has been highlighted the use of biostimulant products, which act to induce plant defenses. Thus, t he objective of this study is to evaluate the interactive effects on the biochemistry and physiology of soybean plants submitted simultaneously to fungicide application and water deficit conditions, and the possible attenuation of plant damage by the biost imulant application, in a controlled environment. Biochemical and physiological changes were evaluated in soybean plants submitted to eight, six, four and two days without irrigation (eight different soil water potentials: - 0.2286, -0.0332, - 0.0245, -0.0164, -0.0074, -0.0029, - 0.0018, and -0.0008 MPa), xenobiotic and biostimulant applications. Visual symptoms showed that under water deficit plants were wilted. Also, under low water availability, was reduced water status, gas exchange, photosynthetic pigments, quantum yield of photosystem II, and increased leaf temperature. Hydrogen peroxide production was increased under deficit water stress and in situ detection of this specie showed high efficiency of biostimulant in its elimination. This was evidenced by the concentration evaluation of the hydrogen peroxide in cells, which decreased by biostimulant application, as well as superoxide. Nearly 70% of membrane damage was observed for plants with less water availability. Lipid peroxidation was increased in plants under low water potentials and under xenobiotic application. Glutathione, component of the metabolism antioxidant non-enzymatic, was also increased in lowest potentials of soil water. Thus, it is possible to conclude that water deficit induced oxidative stress, by the increased production of reactive oxygen species, cellular and molecular damage, and induction of antioxidant defense metabolism, reduction of gas exchange, water status and photosynthetic efficiency. Xenobiotic application also caused changes, but with less intensity. Deleterious effects on the cells were more pronounced when application occurred in plants submitted to low availability of soil water. This indicates different responses to the combination of stresses. The biostimulant application attenuated the effects of water deficit and xenobiotic. Some situations have shown that the xenobiotic can influence the biostimulant action, if applied concomitantly. |
| _version_ | 1871607616990347264 |
|---|---|
| author | Schneider, Julia Renata |
| author_browse | Schneider, Julia Renata |
| author_facet | Schneider, Julia Renata |
| author_role | author |
| collection | Repositório Institucional da UPF |
| dc.contributor.author.fl_str_mv | Schneider, Julia Renata |
| dc.contributor.none.fl_str_mv | Lamas Júnior, Geraldo Luiz Chavarria http://lattes.cnpq.br/4856839440390704 |
| dc.date.none.fl_str_mv | 2019-04-05 2025-06-20T16:41:50Z |
| dc.format.none.fl_str_mv | application/pdf application/pdf |
| dc.identifier.uri.fl_str_mv | SCHNEIDER, Julia Renata. Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante. 2019. 91 f.Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. https://repositorio.upf.br/handle/123456789/9258 |
| dc.language.iso.fl_str_mv | por |
| dc.publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Agronomia |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UPF instname:Universidade de Passo Fundo (UPF) instacron:UPF |
| dc.subject.por.fl_str_mv | Soja Plantas - Efeito dos fungicida Antioxidantes CIENCIAS AGRARIAS::AGRONOMIA |
| dc.title.none.fl_str_mv | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante Biochemistry and physiology of soybean in protected environment under variations of water deficit, xenobiotic and biostimulant |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/masterThesis |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Water deficit stress is the one of main occurrence and the one that most affects plant development. Fungicide application protects plants, but can lead to oxidative stress because it is a xenobiotic, compound strange to the plant. Under field conditions, plants are exposed to combinations of biotic and abiotic stresses, such as combined deficit water and fungicide application. The challenge of scientific research is characterize these processes and create management strategies to mitigate metabolic and economic effects. Also, to minimize the impacts of these stresses has been highlighted the use of biostimulant products, which act to induce plant defenses. Thus, t he objective of this study is to evaluate the interactive effects on the biochemistry and physiology of soybean plants submitted simultaneously to fungicide application and water deficit conditions, and the possible attenuation of plant damage by the biost imulant application, in a controlled environment. Biochemical and physiological changes were evaluated in soybean plants submitted to eight, six, four and two days without irrigation (eight different soil water potentials: - 0.2286, -0.0332, - 0.0245, -0.0164, -0.0074, -0.0029, - 0.0018, and -0.0008 MPa), xenobiotic and biostimulant applications. Visual symptoms showed that under water deficit plants were wilted. Also, under low water availability, was reduced water status, gas exchange, photosynthetic pigments, quantum yield of photosystem II, and increased leaf temperature. Hydrogen peroxide production was increased under deficit water stress and in situ detection of this specie showed high efficiency of biostimulant in its elimination. This was evidenced by the concentration evaluation of the hydrogen peroxide in cells, which decreased by biostimulant application, as well as superoxide. Nearly 70% of membrane damage was observed for plants with less water availability. Lipid peroxidation was increased in plants under low water potentials and under xenobiotic application. Glutathione, component of the metabolism antioxidant non-enzymatic, was also increased in lowest potentials of soil water. Thus, it is possible to conclude that water deficit induced oxidative stress, by the increased production of reactive oxygen species, cellular and molecular damage, and induction of antioxidant defense metabolism, reduction of gas exchange, water status and photosynthetic efficiency. Xenobiotic application also caused changes, but with less intensity. Deleterious effects on the cells were more pronounced when application occurred in plants submitted to low availability of soil water. This indicates different responses to the combination of stresses. The biostimulant application attenuated the effects of water deficit and xenobiotic. Some situations have shown that the xenobiotic can influence the biostimulant action, if applied concomitantly. |
| eu_rights_str_mv | openAccess |
| format | masterThesis |
| id | UPF_1f37a45eeb710152dfd6ac276bd591b4 |
| identifier_str_mv | SCHNEIDER, Julia Renata. Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante. 2019. 91 f.Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019. |
| instacron_str | UPF |
| institution | UPF |
| instname_str | Universidade de Passo Fundo (UPF) |
| language | por |
| network_acronym_str | UPF |
| network_name_str | Repositório Institucional da UPF |
| oai_identifier_str | oai:repositorio.upf.br:123456789/9258 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher.none.fl_str_mv | Universidade de Passo Fundo Faculdade de Agronomia e Medicina Veterinária – FAMV Brasil UPF Programa de Pós-Graduação em Agronomia |
| reponame_str | Repositório Institucional da UPF |
| repository.mail.fl_str_mv | jucelei@upf.br||biblio@upf.br |
| repository.name.fl_str_mv | Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| repository_id_str | 1610 |
| spelling | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulanteBiochemistry and physiology of soybean in protected environment under variations of water deficit, xenobiotic and biostimulantSojaPlantas - Efeito dos fungicidaAntioxidantesCIENCIAS AGRARIAS::AGRONOMIAWater deficit stress is the one of main occurrence and the one that most affects plant development. Fungicide application protects plants, but can lead to oxidative stress because it is a xenobiotic, compound strange to the plant. Under field conditions, plants are exposed to combinations of biotic and abiotic stresses, such as combined deficit water and fungicide application. The challenge of scientific research is characterize these processes and create management strategies to mitigate metabolic and economic effects. Also, to minimize the impacts of these stresses has been highlighted the use of biostimulant products, which act to induce plant defenses. Thus, t he objective of this study is to evaluate the interactive effects on the biochemistry and physiology of soybean plants submitted simultaneously to fungicide application and water deficit conditions, and the possible attenuation of plant damage by the biost imulant application, in a controlled environment. Biochemical and physiological changes were evaluated in soybean plants submitted to eight, six, four and two days without irrigation (eight different soil water potentials: - 0.2286, -0.0332, - 0.0245, -0.0164, -0.0074, -0.0029, - 0.0018, and -0.0008 MPa), xenobiotic and biostimulant applications. Visual symptoms showed that under water deficit plants were wilted. Also, under low water availability, was reduced water status, gas exchange, photosynthetic pigments, quantum yield of photosystem II, and increased leaf temperature. Hydrogen peroxide production was increased under deficit water stress and in situ detection of this specie showed high efficiency of biostimulant in its elimination. This was evidenced by the concentration evaluation of the hydrogen peroxide in cells, which decreased by biostimulant application, as well as superoxide. Nearly 70% of membrane damage was observed for plants with less water availability. Lipid peroxidation was increased in plants under low water potentials and under xenobiotic application. Glutathione, component of the metabolism antioxidant non-enzymatic, was also increased in lowest potentials of soil water. Thus, it is possible to conclude that water deficit induced oxidative stress, by the increased production of reactive oxygen species, cellular and molecular damage, and induction of antioxidant defense metabolism, reduction of gas exchange, water status and photosynthetic efficiency. Xenobiotic application also caused changes, but with less intensity. Deleterious effects on the cells were more pronounced when application occurred in plants submitted to low availability of soil water. This indicates different responses to the combination of stresses. The biostimulant application attenuated the effects of water deficit and xenobiotic. Some situations have shown that the xenobiotic can influence the biostimulant action, if applied concomitantly.Estresse por déficit hídrico é o de principal ocorrência e que mais afeta o desenvolvimento das plantas. A aplicação de fungicida protege as plantas, mas pode acarretar em estresse oxidativo por ser um xenobiótico, composto estranho à planta. Em condições de campo, as plantas estão expostas a combinações de estresses bióticos e abióticos, como pode ser o caso do déficit hídrico combinado com a aplicação de fungicida. O desafio da pesquisa científica é caracterizar estes processos e criar estratégias de manejo para mitigar o efeito metabólico e econômico. Ainda, para minimizar os impactos destes estresses, vem se destacando o uso de produtos bioestimulantes, que atuam induzindo as defesas das plantas. Assim, o objetivo deste estudo é avaliar os efeitos interativos na bioquímica e fisiologia de plantas de soja submetidas simultaneamente à aplicação de fungicida e a condições de déficit hídrico, e a possível atenuação de danos às plantas pela aplicação de bioestimulante, em ambiente protegido. Foram avaliados alterações bioquímicas e fisiológicas em plantas de soja submetidas a restrição de água durante oito, seis, quatro e dois dias sem irrigação (oito potenciais da água do solo diferentes: -0,2286; -0,0332; -0,0245; -0,0164; -0,0074; -0,0029; -0,0018; -0,0008 MPa), aplicação de xenobiótico e bioestimulante. Sintomas visuais foram observados com relação à redução na turgescência das plantas sob restrição de água. Também sob baixa disponibilidade de água, o status hídrico foi prejudicado, assim como diminuída a troca de gases, os pigmentos fotossintéticos e o rendimento quântico do fotossistema II, e aumentada a temperatura foliar. A produção de peróxido de hidrogênio aumentou sob estresse de déficit hídrico, e a detecção in situ dessa espécie reativa mostrou alta eficiência do bioestimulante na sua eliminação. Isso foi comprovado pela avaliação da concentração do peróxido de hidrogênio nas células, a qual diminuiu pela aplicação de bioestimulante, assim como o superóxido. Danos de membrana de quase 70% foram observadas para as plantas sob menor disponibilidade de água. A peroxidação lipídica foi aumentada em plantas sob baixos potenciais da água e sob aplicação de xenobiótico. A glutationa, componente do metabolismo antioxidante não enzimático, também foi aumentada nos menores potenciais da água do solo. Assim, é possível concluir que o déficit hídrico induziu ao estresse oxidativo, pelo aumento da produção de espécies reativas de oxigênio, danos celulares e moleculares, indução do metabolismo de defesa antioxidante, diminuição da troca de gases, do status hídrico e da eficiência fotossintética. A aplicação de xenobiótico também causou alterações, mas com menor intensidade. Efeitos deletérios às células foram mais pronunciados quando a aplicação ocorreu nas plantas submetidas à condição de baixa disponibilidade de água do solo. Isso indica diferentes respostas para a combinação de estresses. A aplicação de bioestimulante amenizou os efeitos do déficit hídrico e do xenobiótico. Algumas situações mostraram que o xenobiótico pode influenciar a ação do bioestimulante se aplicados concomitantemente.Universidade de Passo FundoFaculdade de Agronomia e Medicina Veterinária – FAMVBrasilUPFPrograma de Pós-Graduação em AgronomiaLamas Júnior, Geraldo Luiz Chavarriahttp://lattes.cnpq.br/4856839440390704Schneider, Julia Renata2025-06-20T16:41:50Z2019-04-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSCHNEIDER, Julia Renata. Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante. 2019. 91 f.Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo, RS, 2019.https://repositorio.upf.br/handle/123456789/9258porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UPFinstname:Universidade de Passo Fundo (UPF)instacron:UPF2025-12-30T20:29:55Zoai:repositorio.upf.br:123456789/9258Repositório InstitucionalPRIhttp://repositorio.upf.br/oai/requestjucelei@upf.br||biblio@upf.bropendoar:16102025-12-30T20:29:55Repositório Institucional da UPF - Universidade de Passo Fundo (UPF) |
| spellingShingle | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante Schneider, Julia Renata Soja Plantas - Efeito dos fungicida Antioxidantes CIENCIAS AGRARIAS::AGRONOMIA |
| status_str | publishedVersion |
| title | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| title_full | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| title_fullStr | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| title_full_unstemmed | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| title_short | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| title_sort | Bioquímica e fisiologia da soja em ambiente protegido sob variações de déficit hídrico, xenobiótico e bioestimulante |
| topic | Soja Plantas - Efeito dos fungicida Antioxidantes CIENCIAS AGRARIAS::AGRONOMIA |
| url | https://repositorio.upf.br/handle/123456789/9258 |