Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism
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| Autor principal: | |
|---|---|
| Data de publicació: | 2020 |
| Altres autors: | , , , , , , , |
| Format: | Article |
| Idioma: | eng |
| Font: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/39625 https://doi.org/10.3390/toxins12090550 https://orcid.org/0000-0002-5901-0911 https://orcid.org/0000-0001-9075-7725 |
Sumari: | Parkinson’s disease (PD) is a progressive neurodegenerative condition that affects the Central Nervous System (CNS). Insect venoms show high molecular variability and selectivity in the CNS of mammals and present potential for the development of new drugs for the treatment of PD. In this study, we isolated and identified a component of the venom of the social wasp Parachartergus fraternus and evaluated its neuroprotective activity in the murine model of PD. For this purpose, the venom was filtered and separated through HPLC; fractions were analyzed through mass spectrometry and the active fraction was identified as a novel peptide, called Fraternine. We performed two behavioral tests to evaluate motor discoordination, as well as an apomorphine-induced rotation test. We also conducted an immunohistochemical assay to assess protection in TH+ neurons in the Substantia Nigra (SN) region. Group treated with 10 µg/animal of Fraternine remained longer in the rotarod compared to the lesioned group. In the apomorphine test, Fraternine decreased the number of rotations between treatments. This dose also inhibited dopaminergic neuronal loss, as indicated by immunohistochemical analysis. This study identified a novel peptide able to prevent the death of dopaminergic neurons of the SN and recover motor deficit in a 6-OHDA-induced murine model of PD. |
| _version_ | 1871442593674428416 |
|---|---|
| author | Mayer, Andréia Biolchi |
| author2 | Oliveira, Danilo Gustavo Rodrigues de Amaral, Henrique de Oliveira Campos, Gabriel Avohay Alves Gonçalves, Jacqueline Coimbra Souza, Adolfo Carlos Barros de Lima, Marcos Robalinho Silva, Luciano Paulino Mortari, Márcia Renata |
| author2_role | author author author author author author author author |
| author_browse | Amaral, Henrique de Oliveira Campos, Gabriel Avohay Alves Gonçalves, Jacqueline Coimbra Lima, Marcos Robalinho Mayer, Andréia Biolchi Mortari, Márcia Renata Oliveira, Danilo Gustavo Rodrigues de Silva, Luciano Paulino Souza, Adolfo Carlos Barros de |
| author_facet | Mayer, Andréia Biolchi Oliveira, Danilo Gustavo Rodrigues de Amaral, Henrique de Oliveira Campos, Gabriel Avohay Alves Gonçalves, Jacqueline Coimbra Souza, Adolfo Carlos Barros de Lima, Marcos Robalinho Silva, Luciano Paulino Mortari, Márcia Renata |
| author_role | author |
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| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Mayer, Andréia Biolchi Oliveira, Danilo Gustavo Rodrigues de Amaral, Henrique de Oliveira Campos, Gabriel Avohay Alves Gonçalves, Jacqueline Coimbra Souza, Adolfo Carlos Barros de Lima, Marcos Robalinho Silva, Luciano Paulino Mortari, Márcia Renata |
| dc.date.accessioned.fl_str_mv | 2020-11-11T18:28:52Z |
| dc.date.available.fl_str_mv | 2020-11-11T18:28:52Z |
| dc.date.issued.fl_str_mv | 2020-08-27 |
| dc.identifier.citation.fl_str_mv | BIOLCH, Andréia Mayer et al. Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism. Toxins, v. 12, n. 9, 550, 2020. DOI: https://doi.org/10.3390/toxins12090550. Disponível em: https://www.mdpi.com/2072-6651/12/9/550. Acesso em: 11 nov. 2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/toxins12090550 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0002-5901-0911 https://orcid.org/0000-0001-9075-7725 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/39625 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Vespa - veneno Capacidade motora Parkinson, Doença de |
| dc.title.pt_BR.fl_str_mv | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Parkinson’s disease (PD) is a progressive neurodegenerative condition that affects the Central Nervous System (CNS). Insect venoms show high molecular variability and selectivity in the CNS of mammals and present potential for the development of new drugs for the treatment of PD. In this study, we isolated and identified a component of the venom of the social wasp Parachartergus fraternus and evaluated its neuroprotective activity in the murine model of PD. For this purpose, the venom was filtered and separated through HPLC; fractions were analyzed through mass spectrometry and the active fraction was identified as a novel peptide, called Fraternine. We performed two behavioral tests to evaluate motor discoordination, as well as an apomorphine-induced rotation test. We also conducted an immunohistochemical assay to assess protection in TH+ neurons in the Substantia Nigra (SN) region. Group treated with 10 µg/animal of Fraternine remained longer in the rotarod compared to the lesioned group. In the apomorphine test, Fraternine decreased the number of rotations between treatments. This dose also inhibited dopaminergic neuronal loss, as indicated by immunohistochemical analysis. This study identified a novel peptide able to prevent the death of dopaminergic neurons of the SN and recover motor deficit in a 6-OHDA-induced murine model of PD. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_eba8c53e8a6b290ca623bddcb7a5e853 |
| identifier_str_mv | BIOLCH, Andréia Mayer et al. Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism. Toxins, v. 12, n. 9, 550, 2020. DOI: https://doi.org/10.3390/toxins12090550. Disponível em: https://www.mdpi.com/2072-6651/12/9/550. Acesso em: 11 nov. 2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/39625 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Mayer, Andréia BiolchiOliveira, Danilo Gustavo Rodrigues deAmaral, Henrique de OliveiraCampos, Gabriel Avohay AlvesGonçalves, Jacqueline CoimbraSouza, Adolfo Carlos Barros deLima, Marcos RobalinhoSilva, Luciano PaulinoMortari, Márcia Renata2020-11-11T18:28:52Z2020-11-11T18:28:52Z2020-08-27BIOLCH, Andréia Mayer et al. Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism. Toxins, v. 12, n. 9, 550, 2020. DOI: https://doi.org/10.3390/toxins12090550. Disponível em: https://www.mdpi.com/2072-6651/12/9/550. Acesso em: 11 nov. 2020.https://repositorio.unb.br/handle/10482/39625https://doi.org/10.3390/toxins12090550https://orcid.org/0000-0002-5901-0911https://orcid.org/0000-0001-9075-7725MDPI© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/openAccessFraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVespa - venenoCapacidade motoraParkinson, Doença deParkinson’s disease (PD) is a progressive neurodegenerative condition that affects the Central Nervous System (CNS). Insect venoms show high molecular variability and selectivity in the CNS of mammals and present potential for the development of new drugs for the treatment of PD. In this study, we isolated and identified a component of the venom of the social wasp Parachartergus fraternus and evaluated its neuroprotective activity in the murine model of PD. For this purpose, the venom was filtered and separated through HPLC; fractions were analyzed through mass spectrometry and the active fraction was identified as a novel peptide, called Fraternine. We performed two behavioral tests to evaluate motor discoordination, as well as an apomorphine-induced rotation test. We also conducted an immunohistochemical assay to assess protection in TH+ neurons in the Substantia Nigra (SN) region. Group treated with 10 µg/animal of Fraternine remained longer in the rotarod compared to the lesioned group. In the apomorphine test, Fraternine decreased the number of rotations between treatments. This dose also inhibited dopaminergic neuronal loss, as indicated by immunohistochemical analysis. This study identified a novel peptide able to prevent the death of dopaminergic neurons of the SN and recover motor deficit in a 6-OHDA-induced murine model of PD.Faculdade de Medicina (FM)engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBLICENSElicense.txtlicense.txttext/plain163http://repositorio.unb.br/bitstream/10482/39625/2/license.txtba54f8d1c5f5ec8df897ad678916c701MD52open accessORIGINALARTIGO_FraternineNovelWasp.pdfARTIGO_FraternineNovelWasp.pdfapplication/pdf2708067http://repositorio.unb.br/bitstream/10482/39625/1/ARTIGO_FraternineNovelWasp.pdf458f5fcb15191014b3183726578df601MD51open access10482/396252025-01-15 14:07:54.811open accessoai:repositorio.unb.br:10482/39625U3VibWlzc8OjbyBlZmV0aXZhZGEgcG9yIGludGVncmFudGUgZGEgZXF1aXBlIGRvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuQiBkZSBhY29yZG8gY29tIGxpY2Vuw6dhIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2025-01-15T17:07:54Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism Mayer, Andréia Biolchi Vespa - veneno Capacidade motora Parkinson, Doença de |
| status_str | publishedVersion |
| title | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| title_full | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| title_fullStr | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| title_full_unstemmed | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| title_short | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| title_sort | Fraternine, a novel wasp peptide, protects against motor impairments in 6-OHDA model of Parkinsonism |
| topic | Vespa - veneno Capacidade motora Parkinson, Doença de |
| url | https://repositorio.unb.br/handle/10482/39625 https://doi.org/10.3390/toxins12090550 https://orcid.org/0000-0002-5901-0911 https://orcid.org/0000-0001-9075-7725 |