Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera
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| Auteur principal: | |
|---|---|
| Date de publication: | 1998 |
| Autres auteurs: | , , , , , , , |
| Format: | Article |
| Langue: | eng |
| Source: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/25456 https://dx.doi.org/10.1590/S0100-879X1998001000015 |
Résumé: | A neurotoxic peptide, granulitoxin (GRX), was isolated from the sea anemone Bunodosoma granulifera. The N-terminal amino acid sequence of GRX is AKTGILDSDGPTVAGNSLSGT and its molecular mass is 4958 Da by electrospray mass spectrometry. This sequence presents a partial degree of homology with other toxins from sea anemones such as Bunodosoma caissarum, Anthopleura fuscoviridis and Anemonia sulcata. However, important differences were found: the first six amino acids of the sequence are different, Arg-14 was replaced by Ala and no cysteine residues were present in the partial sequence, while two cysteine residues were present in the first 21 amino acids of other toxins described above. Purified GRX injected ip (800 µg/kg) into mice produced severe neurotoxic effects such as circular movements, aggressive behavior, dyspnea, tonic-clonic convulsion and death. The 2-h LD50 of GRX was 400 ± 83 µg/kg. |
| _version_ | 1871442577599758336 |
|---|---|
| author | Santana, A.N.C. |
| author2 | Leite, A.B. França, M.S.F. França, L. Vale, O.C. Cunha, R.B. Ricart, C.A.O. Sousa, M.V. Carvalho, K.M. |
| author2_role | author author author author author author author author |
| author_browse | Carvalho, K.M. Cunha, R.B. França, L. França, M.S.F. Leite, A.B. Ricart, C.A.O. Santana, A.N.C. Sousa, M.V. Vale, O.C. |
| author_facet | Santana, A.N.C. Leite, A.B. França, M.S.F. França, L. Vale, O.C. Cunha, R.B. Ricart, C.A.O. Sousa, M.V. Carvalho, K.M. |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio.unb.br/bitstream/10482/25456/1/3227c.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Santana, A.N.C. Leite, A.B. França, M.S.F. França, L. Vale, O.C. Cunha, R.B. Ricart, C.A.O. Sousa, M.V. Carvalho, K.M. |
| dc.date.accessioned.fl_str_mv | 2017-12-07T04:28:30Z |
| dc.date.available.fl_str_mv | 2017-12-07T04:28:30Z |
| dc.date.issued.fl_str_mv | 1998 |
| dc.identifier.citation.fl_str_mv | Braz J Med Biol Res,v.31,n.10,p.1335-1338,1998 |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1590/S0100-879X1998001000015 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/25456 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Bunodosoma granulifera sea anemones Peptídeos tóxicos |
| dc.title.pt_BR.fl_str_mv | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | A neurotoxic peptide, granulitoxin (GRX), was isolated from the sea anemone Bunodosoma granulifera. The N-terminal amino acid sequence of GRX is AKTGILDSDGPTVAGNSLSGT and its molecular mass is 4958 Da by electrospray mass spectrometry. This sequence presents a partial degree of homology with other toxins from sea anemones such as Bunodosoma caissarum, Anthopleura fuscoviridis and Anemonia sulcata. However, important differences were found: the first six amino acids of the sequence are different, Arg-14 was replaced by Ala and no cysteine residues were present in the partial sequence, while two cysteine residues were present in the first 21 amino acids of other toxins described above. Purified GRX injected ip (800 µg/kg) into mice produced severe neurotoxic effects such as circular movements, aggressive behavior, dyspnea, tonic-clonic convulsion and death. The 2-h LD50 of GRX was 400 ± 83 µg/kg. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_e58b70892ba2e12d27e4e363d9aacdd9 |
| identifier_str_mv | Braz J Med Biol Res,v.31,n.10,p.1335-1338,1998 |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/25456 |
| publishDate | 1998 |
| publishDateSort | 1998 |
| publisher.none.fl_str_mv | Associação Brasileira de Divulgação Científica |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Santana, A.N.C.Leite, A.B.França, M.S.F.França, L.Vale, O.C.Cunha, R.B.Ricart, C.A.O.Sousa, M.V.Carvalho, K.M.2017-12-07T04:28:30Z2017-12-07T04:28:30Z1998Braz J Med Biol Res,v.31,n.10,p.1335-1338,1998http://repositorio.unb.br/handle/10482/25456https://dx.doi.org/10.1590/S0100-879X1998001000015A neurotoxic peptide, granulitoxin (GRX), was isolated from the sea anemone Bunodosoma granulifera. The N-terminal amino acid sequence of GRX is AKTGILDSDGPTVAGNSLSGT and its molecular mass is 4958 Da by electrospray mass spectrometry. This sequence presents a partial degree of homology with other toxins from sea anemones such as Bunodosoma caissarum, Anthopleura fuscoviridis and Anemonia sulcata. However, important differences were found: the first six amino acids of the sequence are different, Arg-14 was replaced by Ala and no cysteine residues were present in the partial sequence, while two cysteine residues were present in the first 21 amino acids of other toxins described above. Purified GRX injected ip (800 µg/kg) into mice produced severe neurotoxic effects such as circular movements, aggressive behavior, dyspnea, tonic-clonic convulsion and death. The 2-h LD50 of GRX was 400 ± 83 µg/kg.Em processamentoAssociação Brasileira de Divulgação CientíficaPartial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granuliferainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBunodosoma granuliferasea anemonesPeptídeos tóxicosinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINAL3227c.pdfapplication/pdf73853http://repositorio.unb.br/bitstream/10482/25456/1/3227c.pdff1bf7b544e35c05557ea94e4ae44ba8dMD51open access10482/254562024-08-28 13:31:52.389open accessoai:repositorio.unb.br:10482/25456Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T16:31:52Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera Santana, A.N.C. Bunodosoma granulifera sea anemones Peptídeos tóxicos |
| status_str | publishedVersion |
| title | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| title_full | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| title_fullStr | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| title_full_unstemmed | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| title_short | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| title_sort | Partial sequence and toxic effects of granulitoxin, a neurotoxic peptide from the sea anemone Bunodosoma granulifera |
| topic | Bunodosoma granulifera sea anemones Peptídeos tóxicos |
| url | http://repositorio.unb.br/handle/10482/25456 https://dx.doi.org/10.1590/S0100-879X1998001000015 |