Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction
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| Datum vydání: | 2010 |
| Další autoři: | , , , , |
| Médium: | Article |
| Jazyk: | eng |
| Zdroj: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/12626 https://dx.doi.org/10.1186/1471-2180-10-241 |
Shrnutí: | Background: Phospholipase B (PLB) has been reported to be one of the virulence factors for human pathogenic fungi and has also been described as necessary for the early events in infection. Based on these data, we investigated the role of PLB in virulence and modulation of the alveolar pulmonary immune response during infection using an in-vitro model of host-pathogen interaction, i.e. Paracoccidioides brasiliensis yeast cells infecting alveolar macrophage (MH-S) cells. Results: The effect of PLB was analyzed using the specific inhibitor alexidine dihydrochloride (0.25 μM), and pulmonary surfactant (100 μg mL-1), during 6 hours of co-cultivation of P. brasiliensis and MH-S cells. Alexidine dihydrochloride inhibited PLB activity by 66% and significantly decreased the adhesion and internalization of yeast cells by MH-S cells. Genes involved in phagocytosis (trl2, cd14) and the inflammatory response (nfkb, tnf-α, il-1β) were down-regulated in the presence of this PLB inhibitor. In contrast, PLB activity and internalization of yeast cells significantly increased in the presence of pulmonary surfactant; under this condition, genes such as clec2 and the pro-inflammatory inhibitor (nkrf) were up-regulated. Also, the pulmonary surfactant did not alter cytokine production, while alexidine dihydrochloride decreased the levels of interleukin-10 (IL-10) and increased the levels of IL-12 and tumor necrosis factor-α (TNF-α). In addition, gene expression analysis of plb1, sod3 and icl1 suggests that P. brasiliensis gene re-programming is effective in facilitating adaptation to this inhospitable environment, which mimics the lung-environment interaction. Conclusion: P. brasiliensis PLB activity is involved in the process of adhesion and internalization of yeast cells at the MH-S cell surface and may enhance virulence and subsequent down-regulation of macrophage activation. |
| _version_ | 1871442505289957376 |
|---|---|
| author | Soares, Deyze Alencar |
| author2 | Andrade, Rosângela Vieira de Silva, Simoneide Sousa Bocca, Anamélia Lorenzetti Felipe, Maria Sueli Soares Silva, Silvana Petrofeza da |
| author2_role | author author author author author |
| author_browse | Andrade, Rosângela Vieira de Bocca, Anamélia Lorenzetti Felipe, Maria Sueli Soares Silva, Silvana Petrofeza da Silva, Simoneide Sousa Soares, Deyze Alencar |
| author_facet | Soares, Deyze Alencar Andrade, Rosângela Vieira de Silva, Simoneide Sousa Bocca, Anamélia Lorenzetti Felipe, Maria Sueli Soares Silva, Silvana Petrofeza da |
| author_role | author |
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| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/12626/1/ARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf http://repositorio2.unb.br/jspui/bitstream/10482/12626/2/license.txt http://repositorio2.unb.br/jspui/bitstream/10482/12626/3/ARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Soares, Deyze Alencar Andrade, Rosângela Vieira de Silva, Simoneide Sousa Bocca, Anamélia Lorenzetti Felipe, Maria Sueli Soares Silva, Silvana Petrofeza da |
| dc.date.accessioned.fl_str_mv | 2013-03-27T19:55:13Z |
| dc.date.available.fl_str_mv | 2013-03-27T19:55:13Z |
| dc.date.issued.fl_str_mv | 2010 |
| dc.identifier.citation.fl_str_mv | SOARES, Deyze Alencar et al. Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction. BMC Microbiology, v. 10, 2010. Disponível em: <http://www.biomedcentral.com/1471-2180/10/241>. Acesso em: 21 mar. 2013. DOI: 10.1186/1471-2180-10-241. |
| dc.identifier.doi.en.fl_str_mv | https://dx.doi.org/10.1186/1471-2180-10-241 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/12626 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | BioMed Central |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.en.fl_str_mv | Paracoccidioides brasiliensis Paracoccidioidomicose Fungos patogênicos |
| dc.title.en.fl_str_mv | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Background: Phospholipase B (PLB) has been reported to be one of the virulence factors for human pathogenic fungi and has also been described as necessary for the early events in infection. Based on these data, we investigated the role of PLB in virulence and modulation of the alveolar pulmonary immune response during infection using an in-vitro model of host-pathogen interaction, i.e. Paracoccidioides brasiliensis yeast cells infecting alveolar macrophage (MH-S) cells. Results: The effect of PLB was analyzed using the specific inhibitor alexidine dihydrochloride (0.25 μM), and pulmonary surfactant (100 μg mL-1), during 6 hours of co-cultivation of P. brasiliensis and MH-S cells. Alexidine dihydrochloride inhibited PLB activity by 66% and significantly decreased the adhesion and internalization of yeast cells by MH-S cells. Genes involved in phagocytosis (trl2, cd14) and the inflammatory response (nfkb, tnf-α, il-1β) were down-regulated in the presence of this PLB inhibitor. In contrast, PLB activity and internalization of yeast cells significantly increased in the presence of pulmonary surfactant; under this condition, genes such as clec2 and the pro-inflammatory inhibitor (nkrf) were up-regulated. Also, the pulmonary surfactant did not alter cytokine production, while alexidine dihydrochloride decreased the levels of interleukin-10 (IL-10) and increased the levels of IL-12 and tumor necrosis factor-α (TNF-α). In addition, gene expression analysis of plb1, sod3 and icl1 suggests that P. brasiliensis gene re-programming is effective in facilitating adaptation to this inhospitable environment, which mimics the lung-environment interaction. Conclusion: P. brasiliensis PLB activity is involved in the process of adhesion and internalization of yeast cells at the MH-S cell surface and may enhance virulence and subsequent down-regulation of macrophage activation. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_ce65d6c36a705f03ce2ded6dd2cfe762 |
| identifier_str_mv | SOARES, Deyze Alencar et al. Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction. BMC Microbiology, v. 10, 2010. Disponível em: <http://www.biomedcentral.com/1471-2180/10/241>. Acesso em: 21 mar. 2013. DOI: 10.1186/1471-2180-10-241. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/12626 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher.none.fl_str_mv | BioMed Central |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Soares, Deyze AlencarAndrade, Rosângela Vieira deSilva, Simoneide SousaBocca, Anamélia LorenzettiFelipe, Maria Sueli SoaresSilva, Silvana Petrofeza da2013-03-27T19:55:13Z2013-03-27T19:55:13Z2010SOARES, Deyze Alencar et al. Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction. BMC Microbiology, v. 10, 2010. Disponível em: <http://www.biomedcentral.com/1471-2180/10/241>. Acesso em: 21 mar. 2013. DOI: 10.1186/1471-2180-10-241.http://repositorio.unb.br/handle/10482/12626https://dx.doi.org/10.1186/1471-2180-10-241Background: Phospholipase B (PLB) has been reported to be one of the virulence factors for human pathogenic fungi and has also been described as necessary for the early events in infection. Based on these data, we investigated the role of PLB in virulence and modulation of the alveolar pulmonary immune response during infection using an in-vitro model of host-pathogen interaction, i.e. Paracoccidioides brasiliensis yeast cells infecting alveolar macrophage (MH-S) cells. Results: The effect of PLB was analyzed using the specific inhibitor alexidine dihydrochloride (0.25 μM), and pulmonary surfactant (100 μg mL-1), during 6 hours of co-cultivation of P. brasiliensis and MH-S cells. Alexidine dihydrochloride inhibited PLB activity by 66% and significantly decreased the adhesion and internalization of yeast cells by MH-S cells. Genes involved in phagocytosis (trl2, cd14) and the inflammatory response (nfkb, tnf-α, il-1β) were down-regulated in the presence of this PLB inhibitor. In contrast, PLB activity and internalization of yeast cells significantly increased in the presence of pulmonary surfactant; under this condition, genes such as clec2 and the pro-inflammatory inhibitor (nkrf) were up-regulated. Also, the pulmonary surfactant did not alter cytokine production, while alexidine dihydrochloride decreased the levels of interleukin-10 (IL-10) and increased the levels of IL-12 and tumor necrosis factor-α (TNF-α). In addition, gene expression analysis of plb1, sod3 and icl1 suggests that P. brasiliensis gene re-programming is effective in facilitating adaptation to this inhospitable environment, which mimics the lung-environment interaction. Conclusion: P. brasiliensis PLB activity is involved in the process of adhesion and internalization of yeast cells at the MH-S cell surface and may enhance virulence and subsequent down-regulation of macrophage activation.BioMed Central© 2010 Soares etal; licensee BioMed Central Ltd. Thisis an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),which permits unrestricted use, distribution, and reproductionin any medium, provided the original work is properly cited. Fonte: http://www.biomedcentral.com/1471-2180/10/241. Acesso em: 21 mar. 2013.info:eu-repo/semantics/openAccessExtracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleParacoccidioides brasiliensisParacoccidioidomicoseFungos patogênicosengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdfARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdfapplication/pdf748806http://repositorio2.unb.br/jspui/bitstream/10482/12626/1/ARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf2a3eb4ce739a668c723bf1dc63cd4446MD51open accessLICENSElicense.txtlicense.txttext/plain257http://repositorio2.unb.br/jspui/bitstream/10482/12626/2/license.txt79dae6f5bb97ea40c3c831943777e4daMD52open accessTEXTARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf.txtARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf.txtExtracted texttext/plain40781http://repositorio2.unb.br/jspui/bitstream/10482/12626/3/ARTIGO_ExtracellularParacoccidioidesBrasiliensis.pdf.txtb2e8622f0c39fbcea338c9ad2d349d89MD53open access10482/126262023-05-24 20:34:37.395open accessoai:repositorio.unb.br:10482/12626TGljZW5zZSBncmFudGVkIGJ5IFJpY2FyZG8gVGF2YXJlcyAocmljYXJkb3NvYXJlc0BiY2UudW5iLmJyKSBvbiAyMDEzLTAzLTIxVDIwOjQ1OjA2WiAoR01UKToKClN1Ym1pc3PDo28gZWZldGl2YWRhIHBvciBpbnRlZ3JhbnRlIGRhIGVxdWlwZSBkbyBSZXBvc2l0w7NyaW8gSW5zdGl0dWNpb25hbCBkYSBVbkIgZGUgYWNvcmRvIGNvbSBsaWNlbsOnYSBjb25jZWRpZGEgcGVsbyBhdXRvciBlL291IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcy4=Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:37Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction Soares, Deyze Alencar Paracoccidioides brasiliensis Paracoccidioidomicose Fungos patogênicos |
| status_str | publishedVersion |
| title | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| title_full | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| title_fullStr | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| title_full_unstemmed | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| title_short | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| title_sort | Extracellular Paracoccidioides brasiliensis phospholipase B involvement in alveolar macrophage interaction |
| topic | Paracoccidioides brasiliensis Paracoccidioidomicose Fungos patogênicos |
| url | http://repositorio.unb.br/handle/10482/12626 https://dx.doi.org/10.1186/1471-2180-10-241 |