In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis
Saved in:
| Main Author: | |
|---|---|
| Publication Date: | 2019 |
| Other Authors: | , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/36476 https://doi.org/10.1590/1678-4685-gmb-2018-0014 http://orcid.org/0000-0003-2978-3689 http://orcid.org/0000-0001-8892-9233 http://orcid.org/0000-0002-1086-4588 http://orcid.org/0000-0003-3803-0339 http://orcid.org/0000-0001-5378-9840 |
Summary: | Eukaryotic cells have different mechanisms of post-transcriptional regulation. Among these mechanisms, microRNAs promote regulation of targets by cleavage or degradation of the mRNA. Fungi of the Paracoccidioides complex are the etiological agents of the main systemic mycosis of Latin America. These fungi present a plasticity to adapt and survive in different conditions, and the presence of microRNAs-like molecules could be part of the mechanisms that provide such plasticity. MicroRNAs produced by the host influence the progression of this mycosis in the lungs besides regulating targets involved in apoptosis in macrophage, activation of T and B cells and the production of cytokines. Therefore, this work analyzed the presence of regions in the genome of this fungus with a potential to encode microRNAs-like molecules. Here we show by analysis of sequence similarity the presence of 18 regions, putatively coding for microRNAs-like molecules in the Paracoccidioides brasiliensis genome. We also described the conservation of dicer and argonaut proteins and the cognate transcripts induced in the yeast parasitic phase. This work represents a starting point for the analysis of the presence of those molecules in the morphological stages of the fungus and their role in fungal development. |
Similar Items: In silico characterization of microRNAs-like sequences in the genome of Paracoccidioides brasiliensis
- Caracterização e análise da expressão de microRNAs-like de Paracoccidioides brasiliensis
- Identificação de microRNAs de macrófagos murinos expressos diferencialmente em resposta à infecção por Paracoccidioides brasiliensis
- Identificação e caracterização de microRNAs no fungo dimórfico Histoplasma capsulatum
- Expressão diferencial de microRNAs em amostras post-mortem de tecido cerebral de pacientes com Doença de Alzheimer
- Caracterização de microRNAs na interação Musa acuminata-Pseudocercospora musae
- Estudo da participação de microRNAs na regulação da resposta imune inata de macrófagos murinos à infecção por Paracoccidioides brasiliensis