Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract
में बचाया:
| मुख्य लेखक: | |
|---|---|
| प्रकाशन तिथि: | 2020 |
| अन्य लेखक: | , , , , , , , |
| स्वरूप: | Article |
| भाषा: | eng |
| स्रोत: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/39621 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
सारांश: | We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. |
| _version_ | 1871442471849820160 |
|---|---|
| author | Tormena, Renata Pascoal Illanes |
| author2 | Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
| author2_role | author author author author author author author author |
| author_browse | Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Mota, Bruna de Fatima Oliveira Rosa, Eliane Vieira Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique Tormena, Renata Pascoal Illanes |
| author_facet | Tormena, Renata Pascoal Illanes Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 |
| bitstream.url.fl_str_mv | http://repositorio2.unb.br/jspui/bitstream/10482/39621/1/ARTIGO_EvaluationAntimicrobialActivity.pdf http://repositorio2.unb.br/jspui/bitstream/10482/39621/2/license.txt |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Tormena, Renata Pascoal Illanes Rosa, Eliane Vieira Mota, Bruna de Fatima Oliveira Chaker, Juliano Alexandre Fagg, Christopher William Freire, Daniel Oliveira Martins, Paula Melo Silva, Izabel Cristina Rodrigues da Sousa, Marcelo Henrique |
| dc.date.accessioned.fl_str_mv | 2020-11-10T17:32:09Z |
| dc.date.available.fl_str_mv | 2020-11-10T17:32:09Z |
| dc.date.issued.fl_str_mv | 2020-06-01 |
| dc.identifier.citation.fl_str_mv | TORMENA, Renata Pascoal Illanes et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, v. 10, 20676-20681, 2020. DOI: https://doi.org/10.1039/D0RA03240A. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A#!divAbstract. Acesso em: 10 nov. 2020. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.1039/D0RA03240A |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/39621 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | The Royal Society of Chemistry |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Atividade antimicrobiana Nanopartículas de prata Ipê-roxo |
| dc.title.pt_BR.fl_str_mv | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | We describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_b7f276e33038d8cfccfcaf003992d4e2 |
| identifier_str_mv | TORMENA, Renata Pascoal Illanes et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, v. 10, 20676-20681, 2020. DOI: https://doi.org/10.1039/D0RA03240A. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A#!divAbstract. Acesso em: 10 nov. 2020. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/39621 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | The Royal Society of Chemistry |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Tormena, Renata Pascoal IllanesRosa, Eliane VieiraMota, Bruna de Fatima OliveiraChaker, Juliano AlexandreFagg, Christopher WilliamFreire, Daniel OliveiraMartins, Paula MeloSilva, Izabel Cristina Rodrigues daSousa, Marcelo Henrique2020-11-10T17:32:09Z2020-11-10T17:32:09Z2020-06-01TORMENA, Renata Pascoal Illanes et al. Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract. RSC Advances, v. 10, 20676-20681, 2020. DOI: https://doi.org/10.1039/D0RA03240A. Disponível em: https://pubs.rsc.org/en/content/articlelanding/2020/RA/D0RA03240A#!divAbstract. Acesso em: 10 nov. 2020.https://repositorio.unb.br/handle/10482/39621https://doi.org/10.1039/D0RA03240Ahttps://orcid.org/0000-0003-3248-1286https://orcid.org/0000-0002-2144-0959https://orcid.org/0000-0001-6072-7832https://orcid.org/0000-0003-1210-8329The Royal Society of ChemistryOpen Access Article - (CC BY-NC) - This article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence.info:eu-repo/semantics/openAccessEvaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extractinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAtividade antimicrobianaNanopartículas de prataIpê-roxoWe describe here a green method for the preparation of silver nanoparticles (AgNPs), by a microwave-assisted synthesis route using Handroanthus impetiginosus underbark extract, with antibacterial activity. After optimizing the synthesis parameters with a Box–Benhken designed experiment, samples were characterized by powder XRD, TEM, UV-Vis spectroscopy, FTIR and zetametry. Using the overall optimized conditions of synthesis – time of reaction 15 min at 200 °C and plant extract/AgNO3 volume ratio equal to 10% – highly crystalline ∼13.4 nm-sized spherical AgNPs in a well-dispersed colloidal state were obtained. It was also proved that the plant extract compounds act as reductant and capping agents during synthesis to functionalize AgNPs, resulting in a negatively charged surface with high values of zeta potential in a wide range of pH, from acidic to alkaline media. Biological activity tests against Staphylococcus aureus and Escherichia coli and cell viability experiments showed that synthesized AgNPs were not toxic to HaCaT mammalian cells and presented a high efficiency against Gram-positive bacteria (S. aureus). This was associated with the synergistic combination of AgNP silver cores with the capping layer containing natural compounds with antimicrobial properties and considered an alternative to the AgNPs commonly obtained from conventional routes that present antibacterial effectiveness preferentially against Gram-negative strains.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALARTIGO_EvaluationAntimicrobialActivity.pdfARTIGO_EvaluationAntimicrobialActivity.pdfapplication/pdf639441http://repositorio2.unb.br/jspui/bitstream/10482/39621/1/ARTIGO_EvaluationAntimicrobialActivity.pdf1e2894c46cc311406ac4568db150a42aMD51open accessLICENSElicense.txtlicense.txttext/plain102http://repositorio2.unb.br/jspui/bitstream/10482/39621/2/license.txtaed4704d04bb260d4decd80db311aaa5MD52open access10482/396212023-06-04 19:25:37.046open accessoai:repositorio.unb.br:10482/39621U3VibWlzc8OjbyBlZmV0aXZhZGEgZGUgYWNvcmRvIGNvbSBsaWNlbsOnYSBjb25jZWRpZGEgcGVsbyBhdXRvciBlL291IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcy4KRepositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-06-04T22:25:37Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract Tormena, Renata Pascoal Illanes Atividade antimicrobiana Nanopartículas de prata Ipê-roxo |
| status_str | publishedVersion |
| title | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_full | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_fullStr | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_full_unstemmed | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_short | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| title_sort | Evaluation of the antimicrobial activity of silver nanoparticles obtained by microwave-assisted green synthesis using Handroanthus impetiginosus (Mart. ex DC.) Mattos underbark extract |
| topic | Atividade antimicrobiana Nanopartículas de prata Ipê-roxo |
| url | https://repositorio.unb.br/handle/10482/39621 https://doi.org/10.1039/D0RA03240A https://orcid.org/0000-0003-3248-1286 https://orcid.org/0000-0002-2144-0959 https://orcid.org/0000-0001-6072-7832 https://orcid.org/0000-0003-1210-8329 |