Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress
সংরক্ষণ করুন:
| প্রধান লেখক: | |
|---|---|
| প্রকাশনার তারিখ: | 2020 |
| অন্যান্য লেখক: | , , , , , , , , , , , , , , , , , , |
| বিন্যাস: | Article |
| ভাষা: | eng |
| সম্পদ: | Repositório Institucional da UnB |
| Download full: | https://repositorio.unb.br/handle/10482/39919 https://doi.org/10.3390/molecules25122786 https://orcid.org/0000-0002-0273-4169 https://orcid.org/0000-0003-1652-6544 https://orcid.org/0000-0003-1863-5701 https://orcid.org/0000-0002-4806-9192 https://orcid.org/0000-0003-1849-5403 https://orcid.org/0000-0003-0286-1974 https://orcid.org/0000-0001-6896-7179 https://orcid.org/0000-0002-8214-4379 https://orcid.org/0000-0001-6717-3772 |
সংক্ষিপ্ত: | Intestinal mucositis, characterized by inflammatory and/or ulcerative processes in the gastrointestinal tract, occurs due to cellular and tissue damage following treatment with 5-fluorouracil (5-FU). Rutin (RUT), a natural flavonoid extracted from Dimorphandra gardneriana, exhibits antioxidant, anti-inflammatory, cytoprotective, and gastroprotective properties. However, the effect of RUT on inflammatory processes in the intestine, especially on mucositis promoted by antineoplastic agents, has not yet been reported. In this study, we investigated the role of RUT on 5-FU-induced experimental intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, RUT-50, RUT-100, RUT-200, Celecoxib (CLX), and CLX + RUT-200 groups. The mice were weighed daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis); malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) concentrations; mast and goblet cell counts; and cyclooxygenase-2 (COX-2) activity, as well as to perform immunohistochemical analyses. RUT treatment (200 mg/kg) prevented 5-FU-induced histopathological changes and reduced oxidative stress by decreasing MDA concentrations and increasing GSH concentrations. RUT attenuated the inflammatory response by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. These results suggest that the COX-2 pathway is one of the underlying protective mechanisms of RUT against 5-FU-induced intestinal mucositis. |
| _version_ | 1871442466302853120 |
|---|---|
| author | Fideles, Lázaro de Sousa |
| author2 | Miranda, João Antônio Leal de Martins, Conceição da Silva Barbosa, Maria Lucianny Lima Pimenta, Helder Bindá Pimentel, Paulo Vitor de Souza Teixeira, Claudio Silva Scafuri, Marina Alves Sampaio Façanha, Samuel de Osterno Barreto, João Erivan Façanha Carvalho, Poliana Moreira de Medeiros Scafuri, Ariel Gustavo Araújo, Joabe Lima Rocha, Jefferson Almeida Vieira, Icaro Gusmão Pinto Ricardo, Nágila Maria Pontes Silva Campelo, Matheus da Silva Ribeiro, Maria Elenir Nobre Pinho Brito, Gerly Anne de Castro Cerqueira, Gilberto Santos |
| author2_role | author author author author author author author author author author author author author author author author author author author |
| author_browse | Araújo, Joabe Lima Barbosa, Maria Lucianny Lima Barreto, João Erivan Façanha Brito, Gerly Anne de Castro Campelo, Matheus da Silva Carvalho, Poliana Moreira de Medeiros Cerqueira, Gilberto Santos Façanha, Samuel de Osterno Fideles, Lázaro de Sousa Martins, Conceição da Silva Miranda, João Antônio Leal de Pimenta, Helder Bindá Pimentel, Paulo Vitor de Souza Ribeiro, Maria Elenir Nobre Pinho Ricardo, Nágila Maria Pontes Silva Rocha, Jefferson Almeida Scafuri, Ariel Gustavo Scafuri, Marina Alves Sampaio Teixeira, Claudio Silva Vieira, Icaro Gusmão Pinto |
| author_facet | Fideles, Lázaro de Sousa Miranda, João Antônio Leal de Martins, Conceição da Silva Barbosa, Maria Lucianny Lima Pimenta, Helder Bindá Pimentel, Paulo Vitor de Souza Teixeira, Claudio Silva Scafuri, Marina Alves Sampaio Façanha, Samuel de Osterno Barreto, João Erivan Façanha Carvalho, Poliana Moreira de Medeiros Scafuri, Ariel Gustavo Araújo, Joabe Lima Rocha, Jefferson Almeida Vieira, Icaro Gusmão Pinto Ricardo, Nágila Maria Pontes Silva Campelo, Matheus da Silva Ribeiro, Maria Elenir Nobre Pinho Brito, Gerly Anne de Castro Cerqueira, Gilberto Santos |
| author_role | author |
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| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Fideles, Lázaro de Sousa Miranda, João Antônio Leal de Martins, Conceição da Silva Barbosa, Maria Lucianny Lima Pimenta, Helder Bindá Pimentel, Paulo Vitor de Souza Teixeira, Claudio Silva Scafuri, Marina Alves Sampaio Façanha, Samuel de Osterno Barreto, João Erivan Façanha Carvalho, Poliana Moreira de Medeiros Scafuri, Ariel Gustavo Araújo, Joabe Lima Rocha, Jefferson Almeida Vieira, Icaro Gusmão Pinto Ricardo, Nágila Maria Pontes Silva Campelo, Matheus da Silva Ribeiro, Maria Elenir Nobre Pinho Brito, Gerly Anne de Castro Cerqueira, Gilberto Santos |
| dc.date.accessioned.fl_str_mv | 2021-01-15T19:12:27Z |
| dc.date.available.fl_str_mv | 2021-01-15T19:12:27Z |
| dc.date.issued.fl_str_mv | 2020-06-17 |
| dc.identifier.citation.fl_str_mv | FIDELES, Lázaro de Sousa et al. Role of rutin in 5-Fluorouracil-induced intestinal mucositis: prevention of histological damage and reduction of inflammation and oxidative stress. Molecules, v. 25, n. 12, 2786, 2020. DOI: https://doi.org/10.3390/molecules25122786. Disponível em: https://www.mdpi.com/1420-3049/25/12/2786. Acesso em: 15 jan. 2021. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/molecules25122786 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0002-0273-4169 https://orcid.org/0000-0003-1652-6544 https://orcid.org/0000-0003-1863-5701 https://orcid.org/0000-0002-4806-9192 https://orcid.org/0000-0003-1849-5403 https://orcid.org/0000-0003-0286-1974 https://orcid.org/0000-0001-6896-7179 https://orcid.org/0000-0002-8214-4379 https://orcid.org/0000-0001-6717-3772 |
| dc.identifier.uri.fl_str_mv | https://repositorio.unb.br/handle/10482/39919 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Flavonóides Inflamação Intestinos |
| dc.title.pt_BR.fl_str_mv | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Intestinal mucositis, characterized by inflammatory and/or ulcerative processes in the gastrointestinal tract, occurs due to cellular and tissue damage following treatment with 5-fluorouracil (5-FU). Rutin (RUT), a natural flavonoid extracted from Dimorphandra gardneriana, exhibits antioxidant, anti-inflammatory, cytoprotective, and gastroprotective properties. However, the effect of RUT on inflammatory processes in the intestine, especially on mucositis promoted by antineoplastic agents, has not yet been reported. In this study, we investigated the role of RUT on 5-FU-induced experimental intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, RUT-50, RUT-100, RUT-200, Celecoxib (CLX), and CLX + RUT-200 groups. The mice were weighed daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis); malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) concentrations; mast and goblet cell counts; and cyclooxygenase-2 (COX-2) activity, as well as to perform immunohistochemical analyses. RUT treatment (200 mg/kg) prevented 5-FU-induced histopathological changes and reduced oxidative stress by decreasing MDA concentrations and increasing GSH concentrations. RUT attenuated the inflammatory response by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. These results suggest that the COX-2 pathway is one of the underlying protective mechanisms of RUT against 5-FU-induced intestinal mucositis. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_b42e2fe127feb916c42fb7873e41dcac |
| identifier_str_mv | FIDELES, Lázaro de Sousa et al. Role of rutin in 5-Fluorouracil-induced intestinal mucositis: prevention of histological damage and reduction of inflammation and oxidative stress. Molecules, v. 25, n. 12, 2786, 2020. DOI: https://doi.org/10.3390/molecules25122786. Disponível em: https://www.mdpi.com/1420-3049/25/12/2786. Acesso em: 15 jan. 2021. |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/39919 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher.none.fl_str_mv | MDPI |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Fideles, Lázaro de SousaMiranda, João Antônio Leal deMartins, Conceição da SilvaBarbosa, Maria Lucianny LimaPimenta, Helder BindáPimentel, Paulo Vitor de SouzaTeixeira, Claudio SilvaScafuri, Marina Alves SampaioFaçanha, Samuel de OsternoBarreto, João Erivan FaçanhaCarvalho, Poliana Moreira de MedeirosScafuri, Ariel GustavoAraújo, Joabe LimaRocha, Jefferson AlmeidaVieira, Icaro Gusmão PintoRicardo, Nágila Maria Pontes SilvaCampelo, Matheus da SilvaRibeiro, Maria Elenir Nobre PinhoBrito, Gerly Anne de CastroCerqueira, Gilberto Santos2021-01-15T19:12:27Z2021-01-15T19:12:27Z2020-06-17FIDELES, Lázaro de Sousa et al. Role of rutin in 5-Fluorouracil-induced intestinal mucositis: prevention of histological damage and reduction of inflammation and oxidative stress. Molecules, v. 25, n. 12, 2786, 2020. DOI: https://doi.org/10.3390/molecules25122786. Disponível em: https://www.mdpi.com/1420-3049/25/12/2786. Acesso em: 15 jan. 2021.https://repositorio.unb.br/handle/10482/39919https://doi.org/10.3390/molecules25122786https://orcid.org/0000-0002-0273-4169https://orcid.org/0000-0003-1652-6544https://orcid.org/0000-0003-1863-5701https://orcid.org/0000-0002-4806-9192https://orcid.org/0000-0003-1849-5403https://orcid.org/0000-0003-0286-1974https://orcid.org/0000-0001-6896-7179https://orcid.org/0000-0002-8214-4379https://orcid.org/0000-0001-6717-3772MDPI© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).info:eu-repo/semantics/openAccessRole of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stressinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFlavonóidesInflamaçãoIntestinosIntestinal mucositis, characterized by inflammatory and/or ulcerative processes in the gastrointestinal tract, occurs due to cellular and tissue damage following treatment with 5-fluorouracil (5-FU). Rutin (RUT), a natural flavonoid extracted from Dimorphandra gardneriana, exhibits antioxidant, anti-inflammatory, cytoprotective, and gastroprotective properties. However, the effect of RUT on inflammatory processes in the intestine, especially on mucositis promoted by antineoplastic agents, has not yet been reported. In this study, we investigated the role of RUT on 5-FU-induced experimental intestinal mucositis. Swiss mice were randomly divided into seven groups: Saline, 5-FU, RUT-50, RUT-100, RUT-200, Celecoxib (CLX), and CLX + RUT-200 groups. The mice were weighed daily. After treatment, the animals were euthanized and segments of the small intestine were collected to evaluate histopathological alterations (morphometric analysis); malondialdehyde (MDA), myeloperoxidase (MPO), and glutathione (GSH) concentrations; mast and goblet cell counts; and cyclooxygenase-2 (COX-2) activity, as well as to perform immunohistochemical analyses. RUT treatment (200 mg/kg) prevented 5-FU-induced histopathological changes and reduced oxidative stress by decreasing MDA concentrations and increasing GSH concentrations. RUT attenuated the inflammatory response by decreasing MPO activity, intestinal mastocytosis, and COX-2 expression. These results suggest that the COX-2 pathway is one of the underlying protective mechanisms of RUT against 5-FU-induced intestinal mucositis.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBLICENSElicense.txtlicense.txttext/plain163http://repositorio2.unb.br/jspui/bitstream/10482/39919/2/license.txtba54f8d1c5f5ec8df897ad678916c701MD52open accessORIGINALARTIGO_RoleRutin5-Fluorouracil-Induced.pdfARTIGO_RoleRutin5-Fluorouracil-Induced.pdfapplication/pdf6497392http://repositorio2.unb.br/jspui/bitstream/10482/39919/1/ARTIGO_RoleRutin5-Fluorouracil-Induced.pdf4ac3b79ccd7d010eba26ade00164579eMD51open access10482/399192023-05-24 20:47:03.218open accessoai:repositorio.unb.br:10482/39919U3VibWlzc8OjbyBlZmV0aXZhZGEgcG9yIGludGVncmFudGUgZGEgZXF1aXBlIGRvIFJlcG9zaXTDs3JpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuQiBkZSBhY29yZG8gY29tIGxpY2Vuw6dhIGNvbmNlZGlkYSBwZWxvIGF1dG9yIGUvb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzLg==Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:47:03Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress Fideles, Lázaro de Sousa Flavonóides Inflamação Intestinos |
| status_str | publishedVersion |
| title | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| title_full | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| title_fullStr | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| title_full_unstemmed | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| title_short | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| title_sort | Role of rutin in 5-Fluorouracil-induced intestinal mucositis : prevention of histological damage and reduction of inflammation and oxidative stress |
| topic | Flavonóides Inflamação Intestinos |
| url | https://repositorio.unb.br/handle/10482/39919 https://doi.org/10.3390/molecules25122786 https://orcid.org/0000-0002-0273-4169 https://orcid.org/0000-0003-1652-6544 https://orcid.org/0000-0003-1863-5701 https://orcid.org/0000-0002-4806-9192 https://orcid.org/0000-0003-1849-5403 https://orcid.org/0000-0003-0286-1974 https://orcid.org/0000-0001-6896-7179 https://orcid.org/0000-0002-8214-4379 https://orcid.org/0000-0001-6717-3772 |