Challenges in the detection of polymyxin resistance : from today to the future
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| Auteur principal: | |
|---|---|
| Date de publication: | 2024 |
| Autres auteurs: | , , , , |
| Format: | Article |
| Langue: | eng |
| Source: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/48340 https://doi.org/10.3390/microorganisms12010101 https://orcid.org/0000-0001-8545-069X https://orcid.org/0000-0001-9975-6470 |
Résumé: | Antimicrobial resistance is known to be one of the greatest global threats to human health, and is one of the main causes of death worldwide. In this scenario, polymyxins are last-resort antibiotics to treat infections caused by multidrug-resistant bacteria. Currently, the reference test to evaluate the susceptibility of isolates to polymyxins is the broth microdilution method; however, this technique has numerous complications and challenges for use in laboratory routines. Several phenotypic methods have been reported as being promising for implementation in routine diagnostics, including the BMD commercial test, rapid polymyxin NP test, polymyxin elution test, culture medium with polymyxins, and the Polymyxin Drop Test, which require materials for use in routines and must be easy to perform. Furthermore, Sensititre®, molecular tests, MALDI-TOF MS, and Raman spectroscopy present reliable results, but the equipment is not found in most microbiology laboratories. In this context, this review discusses the main laboratory methodologies that allow the detection of resistance to polymyxins, elucidating the challenges and perspectives. |
| _version_ | 1871442442052435968 |
|---|---|
| author | Rubens, Rebeca Siqueira |
| author2 | Arruda, Isabel de Souza Andrade Almeida, Rosane Mansan Nóbrega, Yanna Karla de Medeiros Carneiro, Maiara dos Santos Dalmolin, Tanise Vendruscolo |
| author2_role | author author author author author |
| author_browse | Almeida, Rosane Mansan Arruda, Isabel de Souza Andrade Carneiro, Maiara dos Santos Dalmolin, Tanise Vendruscolo Nóbrega, Yanna Karla de Medeiros Rubens, Rebeca Siqueira |
| author_facet | Rubens, Rebeca Siqueira Arruda, Isabel de Souza Andrade Almeida, Rosane Mansan Nóbrega, Yanna Karla de Medeiros Carneiro, Maiara dos Santos Dalmolin, Tanise Vendruscolo |
| author_role | author |
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| collection | Repositório Institucional da UnB |
| dc.contributor.affiliation.pt_BR.fl_str_mv | Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) Centro de Medicina Laboratorial, Farroupilha Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC) |
| dc.contributor.author.fl_str_mv | Rubens, Rebeca Siqueira Arruda, Isabel de Souza Andrade Almeida, Rosane Mansan Nóbrega, Yanna Karla de Medeiros Carneiro, Maiara dos Santos Dalmolin, Tanise Vendruscolo |
| dc.date.accessioned.fl_str_mv | 2024-06-20T13:11:21Z |
| dc.date.available.fl_str_mv | 2024-06-20T13:11:21Z |
| dc.date.issued.fl_str_mv | 2024-01-04 |
| dc.identifier.citation.fl_str_mv | RUBENS, Rebeca Siqueira et al. Challenges in the detection of polymyxin resistance: from today to the future. Microorganisms, v. 12, n. 1, 2024. DOI: https://doi.org/10.3390/microorganisms12010101. Disponível em: https://www.mdpi.com/2076-2607/12/1/101. |
| dc.identifier.doi.pt_BR.fl_str_mv | https://doi.org/10.3390/microorganisms12010101 |
| dc.identifier.orcid.pt_BR.fl_str_mv | https://orcid.org/0000-0001-8545-069X https://orcid.org/0000-0001-9975-6470 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/48340 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | MDPI |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Suscetibilidade a antimicrobianos Polimixinas Bactérias gram-negativas |
| dc.title.pt_BR.fl_str_mv | Challenges in the detection of polymyxin resistance : from today to the future |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Antimicrobial resistance is known to be one of the greatest global threats to human health, and is one of the main causes of death worldwide. In this scenario, polymyxins are last-resort antibiotics to treat infections caused by multidrug-resistant bacteria. Currently, the reference test to evaluate the susceptibility of isolates to polymyxins is the broth microdilution method; however, this technique has numerous complications and challenges for use in laboratory routines. Several phenotypic methods have been reported as being promising for implementation in routine diagnostics, including the BMD commercial test, rapid polymyxin NP test, polymyxin elution test, culture medium with polymyxins, and the Polymyxin Drop Test, which require materials for use in routines and must be easy to perform. Furthermore, Sensititre®, molecular tests, MALDI-TOF MS, and Raman spectroscopy present reliable results, but the equipment is not found in most microbiology laboratories. In this context, this review discusses the main laboratory methodologies that allow the detection of resistance to polymyxins, elucidating the challenges and perspectives. |
| eu_rights_str_mv | openAccess |
| format | article |
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| identifier_str_mv | RUBENS, Rebeca Siqueira et al. Challenges in the detection of polymyxin resistance: from today to the future. Microorganisms, v. 12, n. 1, 2024. DOI: https://doi.org/10.3390/microorganisms12010101. Disponível em: https://www.mdpi.com/2076-2607/12/1/101. |
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| instname_str | Universidade de Brasília (UnB) |
| language | eng |
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| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher.none.fl_str_mv | MDPI |
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| repository.mail.fl_str_mv | repositorio@unb.br |
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| spelling | Rubens, Rebeca SiqueiraArruda, Isabel de Souza AndradeAlmeida, Rosane MansanNóbrega, Yanna Karla de MedeirosCarneiro, Maiara dos SantosDalmolin, Tanise VendruscoloUniversidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)Centro de Medicina Laboratorial, FarroupilhaUniversidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Laboratório de Microbiologia e Imunologia Clínica (LabMIC)2024-06-20T13:11:21Z2024-06-20T13:11:21Z2024-01-04RUBENS, Rebeca Siqueira et al. Challenges in the detection of polymyxin resistance: from today to the future. Microorganisms, v. 12, n. 1, 2024. DOI: https://doi.org/10.3390/microorganisms12010101. Disponível em: https://www.mdpi.com/2076-2607/12/1/101.http://repositorio.unb.br/handle/10482/48340https://doi.org/10.3390/microorganisms12010101https://orcid.org/0000-0001-8545-069Xhttps://orcid.org/0000-0001-9975-6470engMDPI© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).info:eu-repo/semantics/openAccessChallenges in the detection of polymyxin resistance : from today to the futureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSuscetibilidade a antimicrobianosPolimixinasBactérias gram-negativasAntimicrobial resistance is known to be one of the greatest global threats to human health, and is one of the main causes of death worldwide. In this scenario, polymyxins are last-resort antibiotics to treat infections caused by multidrug-resistant bacteria. Currently, the reference test to evaluate the susceptibility of isolates to polymyxins is the broth microdilution method; however, this technique has numerous complications and challenges for use in laboratory routines. Several phenotypic methods have been reported as being promising for implementation in routine diagnostics, including the BMD commercial test, rapid polymyxin NP test, polymyxin elution test, culture medium with polymyxins, and the Polymyxin Drop Test, which require materials for use in routines and must be easy to perform. Furthermore, Sensititre®, molecular tests, MALDI-TOF MS, and Raman spectroscopy present reliable results, but the equipment is not found in most microbiology laboratories. In this context, this review discusses the main laboratory methodologies that allow the detection of resistance to polymyxins, elucidating the challenges and perspectives.Faculdade de Ciências da Saúde (FS)Departamento de Farmácia (FS FAR)reponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBLICENSElicense.txtlicense.txttext/plain102http://repositorio.unb.br/bitstream/10482/48340/2/license.txtaed4704d04bb260d4decd80db311aaa5MD52open accessORIGINALARTIGO_ChallengesDetectionPolymyxin.pdfARTIGO_ChallengesDetectionPolymyxin.pdfapplication/pdf1066985http://repositorio.unb.br/bitstream/10482/48340/1/ARTIGO_ChallengesDetectionPolymyxin.pdf42b42957b777ba449bc99efbad1a83a3MD51open access10482/483402025-02-27 14:22:12.184open accessoai:repositorio.unb.br:10482/48340U3VibWlzc8OjbyBlZmV0aXZhZGEgZGUgYWNvcmRvIGNvbSBsaWNlbsOnYSBjb25jZWRpZGEgcGVsbyBhdXRvciBlL291IGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcy4KRepositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2025-02-27T17:22:12Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Challenges in the detection of polymyxin resistance : from today to the future Rubens, Rebeca Siqueira Suscetibilidade a antimicrobianos Polimixinas Bactérias gram-negativas |
| status_str | publishedVersion |
| title | Challenges in the detection of polymyxin resistance : from today to the future |
| title_full | Challenges in the detection of polymyxin resistance : from today to the future |
| title_fullStr | Challenges in the detection of polymyxin resistance : from today to the future |
| title_full_unstemmed | Challenges in the detection of polymyxin resistance : from today to the future |
| title_short | Challenges in the detection of polymyxin resistance : from today to the future |
| title_sort | Challenges in the detection of polymyxin resistance : from today to the future |
| topic | Suscetibilidade a antimicrobianos Polimixinas Bactérias gram-negativas |
| url | http://repositorio.unb.br/handle/10482/48340 https://doi.org/10.3390/microorganisms12010101 https://orcid.org/0000-0001-8545-069X https://orcid.org/0000-0001-9975-6470 |