Challenges in the detection of polymyxin resistance : from today to the future
Kaydedildi:
| Yazar: | |
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| Yayın Tarihi: | 2024 |
| Diğer Yazarlar: | , , , , |
| Materyal Türü: | Article |
| Dil: | eng |
| Kaynak: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/48340 https://doi.org/10.3390/microorganisms12010101 https://orcid.org/0000-0001-8545-069X https://orcid.org/0000-0001-9975-6470 |
Özet: | Antimicrobial resistance is known to be one of the greatest global threats to human health, and is one of the main causes of death worldwide. In this scenario, polymyxins are last-resort antibiotics to treat infections caused by multidrug-resistant bacteria. Currently, the reference test to evaluate the susceptibility of isolates to polymyxins is the broth microdilution method; however, this technique has numerous complications and challenges for use in laboratory routines. Several phenotypic methods have been reported as being promising for implementation in routine diagnostics, including the BMD commercial test, rapid polymyxin NP test, polymyxin elution test, culture medium with polymyxins, and the Polymyxin Drop Test, which require materials for use in routines and must be easy to perform. Furthermore, Sensititre®, molecular tests, MALDI-TOF MS, and Raman spectroscopy present reliable results, but the equipment is not found in most microbiology laboratories. In this context, this review discusses the main laboratory methodologies that allow the detection of resistance to polymyxins, elucidating the challenges and perspectives. |