Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil
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| Autor principal: | |
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| Data de publicació: | 2008 |
| Altres autors: | , , , , , , , , |
| Format: | Article |
| Idioma: | eng |
| Font: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/27170 https://dx.doi.org/10.1590/S0074-02762008000300011 |
Sumari: | Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development. |
| _version_ | 1871442439114326016 |
|---|---|
| author | Tavares, Talissa de Moraes |
| author2 | Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
| author2_role | author author author author author author author author author |
| author_browse | Andreasi, Márcia Sueli Assis Brito, Wilia Marta Elsner Diederichsen de Cardoso, Divina das Dôres de Paula Costa, Paulo Sérgio Sucasas da Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Giugliano, Loreny Gimenes Parente, Juliana Alves Soares, Célia Maria Almeida Tavares, Talissa de Moraes |
| author_facet | Tavares, Talissa de Moraes Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | http://repositorio.unb.br/bitstream/10482/27170/1/304.pdf |
| collection | Repositório Institucional da UnB |
| dc.contributor.author.fl_str_mv | Tavares, Talissa de Moraes Brito, Wilia Marta Elsner Diederichsen de Fiaccadori, Fabíola Souza Freitas, Erika Regina Leal de Parente, Juliana Alves Costa, Paulo Sérgio Sucasas da Giugliano, Loreny Gimenes Andreasi, Márcia Sueli Assis Soares, Célia Maria Almeida Cardoso, Divina das Dôres de Paula |
| dc.date.accessioned.fl_str_mv | 2017-12-07T04:49:23Z |
| dc.date.available.fl_str_mv | 2017-12-07T04:49:23Z |
| dc.date.issued.fl_str_mv | 2008 |
| dc.identifier.citation.fl_str_mv | Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008 |
| dc.identifier.doi.pt_BR.fl_str_mv | https://dx.doi.org/10.1590/S0074-02762008000300011 |
| dc.identifier.uri.fl_str_mv | http://repositorio.unb.br/handle/10482/27170 |
| dc.language.iso.fl_str_mv | eng |
| dc.publisher.none.fl_str_mv | Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.rights.driver.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
| dc.subject.keyword.pt_BR.fl_str_mv | Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
| dc.title.pt_BR.fl_str_mv | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| dc.type.driver.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | UNB_a5b3f0da2dcacd450c2faa2364eb9a87 |
| identifier_str_mv | Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008 |
| instacron_str | UNB |
| institution | UNB |
| instname_str | Universidade de Brasília (UnB) |
| language | eng |
| network_acronym_str | UNB |
| network_name_str | Repositório Institucional da UnB |
| oai_identifier_str | oai:repositorio.unb.br:10482/27170 |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher.none.fl_str_mv | Instituto Oswaldo Cruz, Ministério da Saúde |
| reponame_str | Repositório Institucional da UnB |
| repository.mail.fl_str_mv | repositorio@unb.br |
| repository.name.fl_str_mv | Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| repository_id_str | |
| spelling | Tavares, Talissa de MoraesBrito, Wilia Marta Elsner Diederichsen deFiaccadori, Fabíola SouzaFreitas, Erika Regina Leal deParente, Juliana AlvesCosta, Paulo Sérgio Sucasas daGiugliano, Loreny GimenesAndreasi, Márcia Sueli AssisSoares, Célia Maria AlmeidaCardoso, Divina das Dôres de Paula2017-12-07T04:49:23Z2017-12-07T04:49:23Z2008Mem. Inst. Oswaldo Cruz,v.103,n.3,p.288-294,2008http://repositorio.unb.br/handle/10482/27170https://dx.doi.org/10.1590/S0074-02762008000300011Nonstructural protein 4 (NSP4), encoded by group A rotavirus genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. The NSP4 gene has been sequenced, and five distinct genetic groups have been described: genotypes A-E. NSP4 genotypes A, B, and C have been detected in humans. In this study, the NSP4-encoding gene of human rotavirus strains of different G and P genotypes collected from children between 1987 and 2003 in three cities of West Central region of Brazil was characterized. NSP4 gene of 153 rotavirus-positive fecal samples was amplified by reverse transcriptase-polymerase chain reaction and then sequenced. For phylogenetic analysis, NSP4 nucleotide sequences of these samples were compared to nucleotide sequences of reference strains available in GenBank. Two distinct NSP4 genotypes could be identified: 141 (92.2%) sequences clustered with NSP4 genotype B, and 12 sequences (7.8%) clustered with NSP4 genotype A. These results reinforce that further investigations are needed to assess the validity of NSP4 as a suitable target for epidemiologic surveillance of rotavirus infections and vaccine development.Em processamentoInstituto Oswaldo Cruz, Ministério da SaúdeMolecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRotavírus grupo AGene nsp4GenótiposRegião Centro-OesteBrasilinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINAL304.pdfapplication/pdf1004257http://repositorio.unb.br/bitstream/10482/27170/1/304.pdfb01a6a667c681990ec0dcd6ec9539f9fMD51open access10482/271702024-08-28 16:04:09.707open accessoai:repositorio.unb.br:10482/27170Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:09Repositório Institucional da UnB - Universidade de Brasília (UnB) |
| spellingShingle | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil Tavares, Talissa de Moraes Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
| status_str | publishedVersion |
| title | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| title_full | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| title_fullStr | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| title_full_unstemmed | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| title_short | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| title_sort | Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil |
| topic | Rotavírus grupo A Gene nsp4 Genótipos Região Centro-Oeste Brasil |
| url | http://repositorio.unb.br/handle/10482/27170 https://dx.doi.org/10.1590/S0074-02762008000300011 |