Physiological and proteomic analyses of Saccharum spp. grown under salt stress
Guardat en:
| Autor principal: | |
|---|---|
| Data de publicació: | 2014 |
| Altres autors: | , , , , , , |
| Format: | Article |
| Idioma: | eng |
| Font: | Repositório Institucional da UnB |
| Download full: | http://repositorio.unb.br/handle/10482/23814 https://dx.doi.org/10.1371/journal.pone.0098463 |
Sumari: | Sugarcane (Saccharum spp.) is the world most productive sugar producing crop, making an understanding of its stress physiology key to increasing both sugar and ethanol production. To understand the behavior and salt tolerance mechanisms of sugarcane, two cultivars commonly used in Brazilian agriculture, RB867515 and RB855536, were submitted to salt stress for 48 days. Physiological parameters including net photosynthesis, water potential, dry root and shoot mass and malondialdehyde (MDA) content of leaves were determined. Control plants of the two cultivars showed similar values for most traits apart from higher root dry mass in RB867515. Both cultivars behaved similarly during salt stress, except for MDA levels for which there was a delay in the response for cultivar RB867515. Analysis of leaf macro- and micronutrients concentrations was performed and the concentration of Mn2+ increased on day 48 for both cultivars. In parallel, to observe the effects of salt stress on protein levels in leaves of the RB867515 cultivar, two-dimensional gel electrophoresis followed by MS analysis was performed. Four proteins were differentially expressed between control and salt-treated plants. Fructose 1,6-bisphosphate aldolase was down-regulated, a germin-like protein and glyceraldehyde 3 phosphate dehydrogenase showed increased expression levels under salt stress, and heat-shock protein 70 was expressed only in salt-treated plants. These proteins are involved in energy metabolism and defense-related responses and we suggest that they may be involved in protection mechanisms against salt stress in sugarcane. |
Ítems similars: Physiological and proteomic analyses of Saccharum spp. grown under salt stress
- Caracterização de variedades de cana-soca sob diferentes regimes hídricos no Cerrado
- Correlation between tympanic and rectal temperature in marmosets (Callithrix penicillata) under acute stress
- Physiological changes of Arabica coffee under different intensities and durations of water stress in the Brazilian cerrado
- Caracterização estrutural e bioquímica do colmo das espécies brasileiras do gênero Saccharum L. (POACEAE)
- Avaliação morfofisiológica da cana-de-açúcar sob diferentes regimes hídricos
- Genômica de organelas de cana-de-açúcar (Saccharum spp. cultivar RB867515)